Ab3181

Manufactured by Abcam

Ab3181 is a primary antibody product from Abcam. It is a polyclonal antibody developed in rabbits that recognizes a specific target protein. The antibody is purified and provided in a liquid format.

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Lab products found in correlation

Image station 4000r, by Kodak (1 mentions) Immobilon, by Merck Group (1 mentions) Ab1791, by Santa Cruz Biotechnology (1 mentions) Ab10362, by Abcam (1 mentions) Sc 56, by Santa Cruz Biotechnology (1 mentions) Ta503755, by OriGene (1 mentions)

2 protocols using ab3181

Western Blot Analysis of DNA Repair Proteins

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Proteins were transferred to PVDF-membranes (Immobilon, Millipore) and the membranes were blocked in 5% dry milk in PBS-T before incubation with primary antibodies in 5% dry milk. Antibodies against POLβ (ab3181), POLδ (ab10362, Abcam), XRCC1 (ab1838), LIG1 (ab615), LIG3 (ab587), H3 (ab1791), PCNA (SC-56, Santa-Cruz Biotechnology), FEN1 (A300-256, Bethyl Laboratories) and α-UNG2 (TA503755, OriGene) were used. Membranes were incubated in swine α-rabbit and rabbit α-mouse secondary antibodies (Dako Cytomation) diluted 1:5000 in 1% dry milk and visualized in KODAK Image Station 4000R.

Bjørås K.Ø., Sousa M.M., Sharma A., Fonseca D.M., Søgaard C.K., Bjørås M, & Otterlei M. (2017). Monitoring of the spatial and temporal dynamics of BER/SSBR pathway proteins, including MYH, UNG2, MPG, NTH1 and NEIL1-3, during DNA replication. Nucleic Acids Research, 45(14), 8291-8301.

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Pol II and DNA Polymerase Immunoprecipitation

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Pol II and DNA polymerase antibodies were purchased from Abcam (ab817/ab5408 and ab3181, respectively). Pol II antibody recognizes the phosphorylated as well as the unphosphorylated form of Pol II the enzyme. The concentration of antibodies used for immunoprecipitations was 2 µl/ml

Boots J.L., von Pelchrzim F., Weiss A., Zimmermann B., Friesacher T., Radtke M., Żywicki M., Chen D., Matylla-Kulińska K., Zagrovic B, & Schroeder R. (2020). RNA polymerase II-binding aptamers in human ACRO1 satellites disrupt transcription in cis. Transcription, 11(5), 217-229.

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