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Anti klf4 nbp2 24749

Manufactured by Novus Biologicals
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The Anti-KLF4 (NBP2-24749) is a laboratory reagent used for the detection and quantification of the KLF4 (Kruppel Like Factor 4) protein in various biological samples. It is a polyclonal antibody that specifically binds to the KLF4 protein, allowing for its identification and measurement using various analytical techniques.

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Lab products found in correlation

Anti α sma a2547, by Merck Group (1 mentions) Af835, by R&D Systems (1 mentions) Ab168372, by Abcam (1 mentions) Sb203580, by Promega (1 mentions) Cycloheximide c7698, by Merck Group (1 mentions) Mg132 c2211, by Merck Group (1 mentions) Anti klf4, by Abcam (1 mentions) Anti klf4 sc 20691, by Santa Cruz Biotechnology (1 mentions) Sb239063, by Selleck Chemicals (1 mentions) Anti gst, by Merck Group (1 mentions) Anti ha sc 805, by Santa Cruz Biotechnology (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Glutathione sepharose 4b, by GE Healthcare (1 mentions)

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NBP2-24749 (2 citations)

2 protocols using anti klf4 nbp2 24749

1

Aortic Root Immunohistochemistry Staining

Cited 1 time
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Frozen sections of the aortic root were fixed in ice-cold acetone for 10 min, dried under a ventilator, and washed with PBS. Sections were blocked in 4% FCS with Avidin D solution (Avidin/Biotin Blocking Kit; Vector Laboratories) for 30 min. Primary antibodies were anti-mouse macrophages/monocytes (MCA519GT, Serotec), anti- active caspase 3 (AF835, R&D Systems), anti- α-SMA (A2547, Sigma-Aldrich), and anti- KLF4 (NBP2-24749, Novus Biological). Biotinylated secondary antibodies, ABC Kit Vectastain Elite, and DAB substrate (PerkinElmer, Vector, and Dako) were used. After counterstaining with haematoxylin sections were mounted with Entellon (MERCK) mounting medium. Stained area was measured using Adobe Photoshop or QuPath44 (link).
Imai T., Van T.M., Pasparakis M, & Polykratis A. (2022). Smooth muscle cell specific NEMO deficiency inhibits atherosclerosis in ApoE−/− mice. Scientific Reports, 12, 12538.
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2

Western Blot and IP Antibodies

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The following antibodies were used in western blot: anti-FBXO32 (NBP1-36955, Novus, Littleton, CO, USA); anti-FBXO32 (ab168372, Abcam, Cambridge, MA, USA); anti-KLF4 (sc-20691, Santa-Cruz, Dallas, TX, USA); anti-KLF4 (ab75486, Abcam); anti-ubiquitin (3936, CST, Billerica, MA, USA); anti-HA (sc-805, Santa-Cruz); anti-GST (G7781, Sigma, St Louis, MO, USA); anti-Flag (F7425, Sigma), anti-Myc (562, MBL, Woburn, MA, USA). The following antibodies were used in immunohistochemistry and immunofluorescent double staining: anti-FBXO32 (LS-B9748, Lifespan Biosciences, Seattle, WA, USA) and anti-KLF4 (NBP2-24749, Novus). The anti-KLF4 antibody (12173, CST) and anti-FLAG M2 Affinity Gel (A2220, Sigma) were used for IP. The Glutathione Sepharose 4B (17-0756-01, GE Healthcare, Amersham, UK) was used in GST pulldown. Cycloheximide (C7698) and MG132 (C2211) were purchased from Sigma. SB203580 was obtained from Promega (Madison, WI, USA) and SB239063 was purchased from Selleckchem (Houston, TX, USA).
Zhou H., Liu Y., Zhu R., Ding F., Wan Y., Li Y, & Liu Z. (2017). FBXO32 suppresses breast cancer tumorigenesis through targeting KLF4 to proteasomal degradation. Oncogene, 36(23), 3312-3321.
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