Following deep isoflurane anesthesia, AAV.ChR2-injected animals were transcardially perfused with phosphate buffered saline (PBS) containing 137 mM NaCl, 1.5 mM KH2PO4, 8 mM NaH2PO4, and 2.7 mM KCl (pH = 7.4), followed by 4% paraformaldehyde in PBS. Brains were cryoprotected overnight in 30% sucrose solution and then frozen and embedded in Neg-50 frozen section medium (Richard-Allan Scientific). Coronal slices (30 μm) were taken on a cryostat and directly mounted to the slides. Fluorescence images were then taken with a 10x objective using an Olympus BX61VS slide-scanning microscope.
Bx61vs slide scanning microscope
Manufactured by Olympus
The BX61VS is a slide-scanning microscope manufactured by Olympus. It is designed to capture high-resolution digital images of microscope slides. The core function of the BX61VS is to digitize microscopic samples for storage, analysis, and sharing.
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Lab products found in correlation
2 protocols using bx61vs slide scanning microscope
1
Perfusion, Cryoprotection, and Imaging
2
Perfusion, Cryoprotection, and Imaging
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