Alpha fetoprotein human simplestep elisa kit

Conditioned medium coming from fully differentiated hEBs was collected and stored at −80 °C after 48 hours of the last change of medium. We then quantified albumin secreted from the differentiated embryoid bodies into the culture media using a Human Albumin ELISA kit (Abcam ab108788) following the manufacturer’s instructions. Alpha-fetoprotein secretion quantification assay was performed using an Alpha Fetoprotein Human SimpleStep ELISA kit (Abcam ab193765) according the manufacturer’s instructions. The quantification of Fibrinogen secretion into the culture supernatant was done using a Fibrinogen Human SimpleStep ELISA Kit (abcam – ab171578) following the manufacturer’s instructions. All the samples were carried out in triplicate.

For the Alpha-fetoprotein secretion assay, the quantification was performed using an Alpha Fetoprotein Human SimpleStep ELISA kit (Abcam ab193765) according the manufacturer’s instructions. 50 μL of plasma samples or standard were separately added to appropriate wells followed by 50 μL of Antibody Cocktail. The 96-well plate was sealed and incubated for 1 hour at room temperature on a plate shaker set to 400rpm. The plate was washed according to the routine method. Finally, 50 μL of stop solution was added and absorbance was read at 450 nm. The reference value of circulating AFP in healthy subjects was under 20 ng/mL.

After 24 hours of the last change of medium, conditioned medium coming from fully differentiated hEBs was collected and stored at −80 °C. Albumin secreted from the differentiated embryoid bodies into the culture media was quantified using a Human Albumin ELISA kit (Abcam ab108788) according to the manufacturer’s instructions. For the Alpha-fetoprotein secretion assay the quantification was performed using an Alpha Fetoprotein Human SimpleStep ELISA kit (Abcam ab193765) according the manufacturer’s instructions. The Fibrinogen secretion into the culture supernatant was quantified using a Fibrinogen Human SimpleStep ELISA Kit (abcam–ab171578) following the manufacturer’s instructions. All the samples were carried out in triplicate.