Cisplatin (LKT Laboratories, Inc., St Paul, MN, USA) and oxaliplatin (Wako Pure Chemical Industries, Osaka, Japan) were dissolved in phosphate-buffered saline (PBS, pH 7.4) at a concentration of 1 mM and distilled water at a concentration of 10 mM, respectively, prior to use. Paclitaxel (Sigma-Aldrich, St. Louis, MO, USA; 100 μM) was prepared in dimethyl sulfoxide (DMSO, Nacalai Tesque, Kyoto, Japan) and stored at −80 °C. Each drug solution was diluted in the appropriate culture medium to achieve the desired concentration before use.
Oxaliplatin
Manufactured by Fujifilm
Sourced in Japan
Oxaliplatin is a laboratory chemical used in research applications. It is a type of platinum-based compound that can be used in various scientific experiments and analyses. Oxaliplatin is a versatile tool for researchers, but its specific functions and intended uses are not provided in this description.
Automatically generated - may contain errors
Lab products found in correlation
Cisplatin, by Fujifilm (2 mentions)
Dimethyl sulfoxide (dmso), by Nacalai Tesque (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Cisplatin, by LKT Laboratories (1 mentions)
Cm h2dcfda, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Gemcitabine, by Tokyo Chemical Industry (1 mentions)
Ecl prime, by GE Healthcare (1 mentions)
Actinomycin d, by Fujifilm (1 mentions)
Rpmi 1640 media, by Nacalai Tesque (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 633 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Neurobasal a medium, by Thermo Fisher Scientific (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Anti puromycin antibody, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
11 protocols using oxaliplatin
1
Preparation of Anticancer Drug Solutions
2
Goshajinkigan Protects Against Oxaliplatin-Induced Oxidative Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxaliplatin was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan), and 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) was purchased from Life Technologies (Carlsbad, CA, USA). Goshajinkigan (GJG; TJ-107) is composed of 10 herbal medicines in fixed proportions: Rehmanniae radix 5.0 g, Achyranthis radix 3.0 g, Corni fructus 3.0 g, Moutan cortex 3.0 g, Alismatics rhizome 3.0 g, Dioscoreae rhizome 3.0 g, Plantaginis semen 3.0 g, Hoelen 3.0 g, processed Aconiti tuber 1.0 g, and Cinnamomi cortex 1.0 g. The GJG was prepared as a spray-dried powder from a hot-water extract (Tsumura & Co., Tokyo, Japan). Benzoylaconine, benzoylmesaconine, benzoylhypaconine, 14-anisoylaconine, loganin, morroniside, catalpol and paeoniflorin were obtained from Tsumura & Co. Oxaliplatin was dissolved in 5% glucose solution. GJG was dissolved in distilled water (DW).
3
Chugai Compound Cytotoxicity in Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CBP-93872 (Chugai Pharmaceutical company) was used at a final concentration of 50 μM (HT29) and 200 μM (Panc-1), unless otherwise indicated. Cisplatin (Wako) was used in a final concentration of 30 μM (HT29) and 10 μM (Panc-1), while the final concentrations of oxaliplatin (Wako), 5-FU (Wako), gemcitabine (Tokyo Chemical Industry), nocodazole (Sigma-Aldrich) were 30 μM, 5 μM, 0.1 μM, and 500 nM, respectively. DMSO (Sigma-Aldrich) was used as a control.
4
Multipurpose Immunostaining and Protein Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
0.1% gelatin (Millipore, ES-006-B), actinomycin D (Wako, 018-21264), Alexa Fluor 633 Goat Anti-Mouse IgG (H + L) (life technologies, A-21050), anti-DsRed2 antibody (used at 1:1000 for immunostaining, Clontech, 632496), anti-puromycin antibody (used at 1:1500 for immunostaining or 1:12500 for western blot, Millipore, clone 4G11), B27 supplement (Gibco, 17504-044), bovine serum albumin (Sigma, A3294), CC/Mount™ (Diagnostic BioSystems, K 002), cycloheximide (Nakalai, 66-81-9), cisplatin (Wako, 033-20091), Dulbecco's modified Eagle's medium (DMEM) (Gibco, 12320-032), ECL Prime (GE Healthcare, 72-AS01-20), fetal bovine serum (FBS) (Gibco, 10437-028), GlutaMAX (Gibco, 35050-061), lipofectamine2000 Transfection Reagent (Invitrogen, 11668-019), methotrexate (Wako, 139-13571), neurobasal-A medium (Gibco,10888-022), oxaliplatin (Wako, 156-02691), paraformaldehyde (Sigma, P6148), penicillin-streptomycin (Sigma, P4333), ProLongGold Antifade Reagent with DAPI (Molecular Probes, P-36931), protease inhibitor cocktail (Sigma, P8340), puromycin (Sigma, P8833), RPMI 1640 media (Nacalai, 30264-85), skim milk (BD, 232 100), TRIzol reagent (Life technologies, 15596-018), α-amanitin (Calbiochem, 129741). Antibodies and drugs were stored in small aliquots at RT, 4 or −25°C as recommended by the providers.
5
Intraperitoneal Oxaliplatin Administration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxaliplatin (Wako Pure Chemical Industries, Osaka, Japan) was dissolved in a 5% glucose solution at a concentration of 2 mg/mL and injected intraperitoneally (i.p.) at a dose of 6 mg/kg. The control group received the same volume of 5% glucose solution (i.p.) [17 (link)].
6
Oxaliplatin and Traditional Herbal Medicines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxaliplatin was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). GJG is composed of 10 herbal medicines: Rehmanniae radix (5.0 g), Achyranthis radix (3.0 g), Corni fructus (3.0 g), Moutan cortex (3.0 g), Alismatics rhizome (3.0 g), Dioscoreae rhizome (3.0 g), Plantaginis semen (3.0 g), Hoelen (3.0 g), processed Aconiti tuber (1.0 g), and Cinnamomi cortex (1.0 g). This drug was prepared as a spray-dried powder from a hot-water extract (yield 16%) and obtained from Tsumura & Co. (Tokyo). Bushi (TJ-3023), a processed Aconiti tuber, was also obtained from Tsumura & Co. Oxaliplatin was dissolved in 5% glucose solution. GJG and bushi were dissolved in distilled water.
7
Cytotoxicity Evaluation of Irinotecan and Oxaliplatin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded in microplates (96 wells) at a concentration of 5 × 104 cells/well and 100 μL of culture medium and incubated for 24 h at 37 °C with 5% CO2. Irinotecan (CPT; I6932; Funakoshi, Tokyo, Japan) was dissolved in DMSO and added to the medium (to final concentrations of 0, 0.1, 0.3, 1.0, 3.0, and 10.0 μM). Oxaliplatin (156–02,691; Wako, Tokyo, Japan) was added to the medium (to final concentrations of 0, 0.01, 0.1, 1.0, 10.0, and 100.0 μM). The cell growth reagent for the WST-1 assay was added (10 μL/well) and incubated for 30 min at 37 °C with 5% CO2 after a 48-h incubation with CPT and 24-h of Oxaliplatin. The absorbance of cells between 420 and 480 nm against a blank and background control was measured using a Bio-Rad iMARK Microplate Reader (Bio-Rad Inc. Hercules, CA, USA) .
8
Colorectal Cancer Cell Line Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The colorectal adenocarcinoma cell lines SW620, SW480 and WiDr were obtained from the American Type Culture Collection (Manassas, VA, USA). DLD-1 and HEK293 cell lines were obtained from JCRB Cell Bank (National Institute of Health Sciences, Tokyo, Japan). Caco-2 cells were obtained from the RIKEN Bioresource Center (RIKEN BRC) (Ibaraki, Japan). These cell lines were re-validated by short tandem repeat profiling in 2016 (Promega, Madison, WI, USA). The cells were maintained as described previously.31 (link) Bryostatin 1 and cycloheximide were obtained from Sigma (B7431, C4859) and oxaliplatin was obtained from Wako (156-02691).
9
Oxaliplatin Intraperitoneal Injection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxaliplatin (Wako Pure Chemical Industries, Osaka, Japan) was dissolved in a 5% glucose solution at a concentration of 2 mg/mL and was intraperitoneally injected at a dose of 6 mg/kg [23 (link)]. The same volume of 5% glucose solution was injected to control group.
10
Investigating NF-κB Inhibitors in Oxaliplatin and Curcumin Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxaliplatin and curcumin were purchased from FUJIFILM Wako Pure Chemical. Three known NF‐κB inhibitors (Bay11‐7082; IκBα kinase inhibitor, TPCA1; IκB kinase inhibitor, MG132; proteasome inhibitor) were purchased from Abcam for Bay11‐7082 and TPCA1, or from Cayman Chemical for MG132, respectively. CMG was synthesized using a previous method (KNC Laboratories).21
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!