Cobas e

Manufactured by Roche

Sourced in United States

The Cobas e is a laboratory instrument manufactured by Roche. It is designed for the automated analysis of various clinical samples. The Cobas e performs immunoassay testing, which is a technique used to detect and measure specific substances in a sample.

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Lab products found in correlation

Immulite 2000 xpi, by Siemens (3 mentions) Hiv combi, by Roche (1 mentions) Architect assay, by Abbott (1 mentions) Liaison xl, by DiaSorin (1 mentions) Prolactin 2, by Roche (1 mentions) Contour glucose meter, by Bayer (1 mentions) Cobas c, by Roche (1 mentions) Cobas e601, by Roche (1 mentions) Hydraulic hand dynamometer, by Performance Health (1 mentions) Jamar 5030j1, by Performance Health (1 mentions) M2 basic, by Omron (1 mentions) Alinity i, by Abbott (1 mentions) Immulite 2000, by Siemens (1 mentions) Immulite 2000 analyzer, by Siemens (1 mentions) Cobas 6000, by Roche (1 mentions) I2000 analyzer, by Abbott (1 mentions) Architect scc reagent kit, by Abbott (1 mentions)

Spelling variants of this product

Cobas e 801 analyser (12 citations) Cobas e 411 immunoanalyzer (9 citations) Cobas e-411 auto-analyser (3 citations) Cobas e 601 instrument (3 citations) Roche Cobas e-System (3 citations) Roche cobas e 601 Automated Analyzer (3 citations) Cobas 6000/e 601 module (3 citations) Elecsys Cobas-e-601 analyzer (2 citations) Elecsys Insulin cobas e analyzer (2 citations) Cobas E immunoassay analysers (2 citations)

21 protocols using cobas e

Sudden Death Autopsy HIV Screening

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Blood samples were obtained from decedents who died suddenly and/or unexpectedly. This was done during autopsy, by a forensic pathologist. Sample collection continued until 100 valid samples were analysed. A study and funding limitation was that only 100 samples were to be collected.
The processing of blood samples was done by the following standardised testing procedures developed and used in the NHLS Tshwane virology research department:

After centrifugation of whole blood, the serum was used to run the test in order to minimise the effect that haemolysed blood has on test strips.

The screening test used was the Determine™ HIV-1/2 Ag/Ab Combo assay, and the confirmatory test used was the HIV Combi, Cobas E, Elecsys and Modular (Roche).^{18 (link)}

Morris N.K., du Toit-Prinsloo L., Webber L, & Saayman G. (2016). The prevalence of HIV in the sudden, unexplained and unexpected death population at the Pretoria Medico-Legal Laboratory. Southern African Journal of HIV Medicine, 17(1), 424.

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Biomarker Measurement in Fasted Individuals

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Blood samples were drawn after an overnight fast from the antecubital vein and analyses were performed at the Uppsala University Hospital Clinical Chemistry facility. Glucose was determined with the Architect assay (Abbott, North Chicago, IL, USA). Insulin, cortisol, and C-peptide were determined using Cobas e (Roche, Indianapolis, IN, USA), insulin-like growth factor 1 (IGF-1) with Liaison XL (DiaSorin, Saluggia, Italy) and adrenocorticotropin (ACTH) with Immulite 2000XPi (Siemens Healthcare Global, Erlangen, Germany). Free fatty acids (FFAs) and glycerol were measured using enzymatic assays as previously reported (16 (link),20 (link)). All protocols were followed according to the manufacturer’s instructions.

Almby K.E., Katsogiannos P., Pereira M.J., Karlsson F.A., Sundbom M., Wiklund U., Kamble P.G, & Eriksson J.W. (2021). Time Course of Metabolic, Neuroendocrine, and Adipose Effects During 2 Years of Follow-up After Gastric Bypass in Patients With Type 2 Diabetes. The Journal of Clinical Endocrinology and Metabolism, 106(10), e4049-e4061.

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Metabolic Biomarkers in Clinical Assessment

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Plasma glucose, HbA1c, total cholesterol, triglycerides, HDL-cholesterol, and insulin were measured using HumaStar 600 (Wiesbaden, Germany). LDL-cholesterol was calculated using Friedwald formula. NT-proBNP was measured by electrochemiluminescence immuno-assay using Cobas E (Roche Diagnostics, USA).

Son T.K., Toan N.H., Thang N., Le Trong Tuong H., Tien H.A., Thuy N.H., Van Minh H, & Valensi P. (2022). Prediabetes and insulin resistance in a population of patients with heart failure and reduced or preserved ejection fraction but without diabetes, overweight or hypertension. Cardiovascular Diabetology, 21, 75.

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Metabolic and Hormonal Evaluation Protocol

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Plasma samples were obtained by venipuncture after an overnight fast. Glucose was analyzed based on enzymatic spectrophotometric reactions by an automated analyzer (Hitachi Modular P800, Roche, Basel, Switzerland). Insulin was measured by means of an enzyme-amplified chemiluminescence assay (IMMULITE^®, Diagnostic Products Corp., Los Angeles, CA). The intra-and interassay coefficients of variation (CV) were 4.2% and 5.7%, respectively. Insulin resistance was calculated using the homeostasis model assessment (HOMA-IR) index. Circulating prolactin concentrations were determined by a microparticle chemiluminescent assay (Prolactin II, Elecsys, Cobas E, Roche Diagnostics GmbH., Mannheim, Germany) with a normal range of 1-27 μg/L for women and of 1-20 μg/L for men together with intra- and interassay CV of 2.3 and 5.9%, respectively. Triglycerides, total cholesterol, high-density lipoprotein (HDL)-cholesterol and low-density lipoprotein (LDL)-cholesterol levels were calculated as previously described ^{88 (link)}.

Folgueira C., Beiroa D., Porteiro B., Duquenne M., Puighermanal E., Fondevila M.F., Barja-Fernández S., Gallego R., Hernández-Bautista R., Castelao C., Senra A., Seoane P., Gómez N., Aguiar P., Guallar D., Fidalgo M., Romero-Pico A., Adan R., Blouet C., Labandeira-García J.L., Jeanrenaud F., Kallo I., Liposits Z., Salvador J., Prevot V., Dieguez C., Lopez M., Valjent E., Frühbeck G., Seoane L.M, & Nogueiras R. (2019). Hypothalamic dopamine signaling regulates brown fat thermogenesis. Nature metabolism, 1(8), 811-829.

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Estradiol Quantification via Electrochemiluminescence

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SE levels were measured using an electrochemiluminescence immunoassay with an analyzer (cobas e; Roche Diagnostics, Basel, Switzerland). The estradiol assay employs a competitive test principle using two monoclonal antibodies specifically directed against 17β-estradiol. The limit of detection was set at 18.4–11,010 pmol/L (5–3,000 pg/mL).

Grin L., Berkovitz-Shperling R., Zohav E., Namazov A., Leyetes S, & Friedler S. (2020). Do spontaneously decreasing estradiol levels prior to triggering of ovulation adversely impact in vitro fertilization outcomes?. Clinical and Experimental Reproductive Medicine, 47(3), 213-220.

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Glucose Metabolism Monitoring Protocol

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Glucose levels were measured bedside every 5 minutes during both clamps with a Contour Glucose Meter (Bayer Healthcare, Leverkusen, Germany). In fasting conditions (approximately 8:30 Am) before the clamps and at regular timepoints during both clamps (displayed in Figs. 1 and 2), blood samples for hormonal analyses were obtained from an arterialized vein and were analyzed within 4 hours or frozen at −80 °C. Serum insulin, cortisol, C-peptide (all CobasE, Roche), plasma ACTH, and plasma/serum GH (both Immulite 2000XPi, Siemens Healthcare Global) were analyzed at the Department of Clinical Chemistry, Uppsala University Hospital. Plasma glucagon was measured with ELISA (#10-1271-01, Mercodia, Uppsala, Sweden, within-assay coefficient of variation (CV) 2.1% to 14%, between-assay CV 7.0% to 16%) at the Clinical Diabetes Research Laboratory. Values below the lower limit of quantification (LLQ) for each analyte were imputed to LLQ/2.

Lundqvist M.H., Pereira M.J., Almby K., Hetty S, & Eriksson J.W. (2023). Regulation of the Cortisol Axis, Glucagon, and Growth Hormone by Glucose Is Altered in Prediabetes and Type 2 Diabetes. The Journal of Clinical Endocrinology and Metabolism, 109(2), e675-e688.

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Comprehensive Blood Biomarker Analysis

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Bio-clinical analysis of blood samples was tested for liver profile, kidney profile, glucose level and complete blood count. Liver profile contains two tests: Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT), kidney profile contains two tests: urea, creatinine and glucose level (random blood sugar). Where, the blood samples for the previous tests were collected in serum-coated tubes (Cobas-C & Cobas- E (Roche) 26-instrument, USA). While in EDTA coated tubes (Swelab Alpha Basic, Sweden), blood samples were collected for complete blood count (CBC) which contains four parameters: Red blood cells (RBCs), leucocytes (WBC), platelets (PLT) and hemoglobin (HB). It should be noted that, the blood was centrifuged at 2000xg for 10 min to separate plasma and then stored at − 80 °C until analysis.

Botrous S., Elmaghraby A., El-Achy S., Mustafa Y., Badr E., Haggag A, & Abdel-Rahman S. (2023). Inhibition of TNF-α Oncogene Expression by Artemisia Annua L. Extract Against Pioglitazone Side Effects in Male Albino Mice. Molecular Biotechnology, 66(3), 432-441.

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Thyroid Hormone Measurement Protocol

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TSH, FT4 and FT3 were measured in serum obtained from whole blood samples, collected with 9 mL glass Vacuette® Serum Clot Activator Tubes. We allowed blood to clot and then centrifuged at 1000 × gravitational units (g), and separated serum following a standardized procedure (WHO 2012). We then stored samples at −20 ºC until shipment to Sweden (Department of Clinical Chemistry, Malmö University Hospital, Malmö, Sweden). We analyzed TSH, FT4 and FT3 with a two-step competitive electrochemiluminescence immunoassay method (ECLIA), using Elecsys and Cobas-e immunoassay analyzers (Cobas ® 2017 TSH, FT3, FT4 Roche Diagnostics, Mannheim, Germany). Limit of detections (LODs) were: TSH = 0.005 mIU/L, FT4 = 0.5 pmol/L and FT3 = 0.6 pmol/L.

Vargas A.C., Castañeda J.P., Liljedahl E.R., Mora A.M., Menezes-Filho J.A., Smith D.R., Mergler D., Reich B., Giffin A., Hoppin J.A., Lindh C.H, & van Wendel de Joode B. (2021). Exposure to common-use pesticides, manganese, lead, and thyroid function among pregnant women from the Infants’ Environmental Health (ISA) study, Costa Rica. The Science of the total environment, 810, 151288.

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Serum HE4 Levels in Cancer Patients

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Serum HE4 levels were analyzed at the time of patient diagnosis, during chemotherapy treatment, after its completion and during the follow-up period. Before each sampling, patients were advised not to take any biotin (vitamin B7) supplements as it might have influenced serum HE4 analysis. Laboratory determinations were conducted at the Department of Laboratory Diagnostics SPSK2 in Szczecin, Poland. Roche enzyme immunoassays (Cobas e) were used for determinations. The reference values for HE4 were set at 70.0 pmol/L, respectively. The concentration of HE4 was determined using an “ECLIA” Elecsys electrochemiluminescence method, using the Roche Cobas e 601 analyzer. The range of the measurement was from 15.0 to 1500 pmol/L. Samples with an HE4 concentration above the measuring range were diluted with a Diluent MultiAssay. The determination was performed in accordance to the manufacturers’ protocol. Values below 70 pmol/L were adopted as the limit value of HE4 concentration.

Chudecka-Głaz A., Strojna A., Michalczyk K., Wieder-Huszla S., Safranow K., Skwirczyńska E, & Jurczak A. (2023). Evaluation of He4 Use in the Diagnosis of Ovarian Cancer: First and Second Recurrence, and an Analysis of HE4 Concentration during Second- and Third-Line Chemotherapy. Diagnostics, 13(3), 452.

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Detailed Glycemic Monitoring Protocol

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In all experiments, glucose was sampled every 5 min from an arterialized vein and analyzed with a Contour glucometer (Bayer Healthcare, Leverkusen, Germany). Fasting glucose (hexokinase method) and HbA1c were analyzed at the Department of Clinical Chemistry at UUH. Hormones were sampled at the start and end of the normoglycemic phase and at regular intervals (15–30 min) throughout the hypoglycemic/hyperglycemic phases as depicted in Fig. 1. Hormonal samples were analyzed immediately or frozen at −80°C for later analysis. Insulin, cortisol, and ACTH (except Abrahamsson (11 (link))) were analyzed at the Department of Clinical Chemistry at UUH using immunoassays (CobasE, Roche for insulin and cortisol; Immulite 2000XPi, Siemens Healthcare Global for ACTH). Glucagon was analyzed using an ELISA (#10-1271-01, RRID:AB_2737304, Mercodia, Uppsala, Sweden; within-assay CV 2.1–14%, between-assay CV 7.0–16%) at the Clinical Diabetes and Metabolism Research Laboratory, UU.

Eriksson J.W., Emad R.A., Lundqvist M.H., Abrahamsson N, & Kjellsson M.C. (2023). Altered glucose-dependent secretion of glucagon and ACTH is associated with insulin resistance, assessed by population analysis. Endocrine Connections, 12(4), e220506.

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