Mhcii

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro, United States

The MHCII-/- product is a genetically modified mouse strain that lacks the major histocompatibility complex class II (MHCII) genes. This strain is commonly used in immunology research to study the role of MHCII molecules in immune responses.

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11 protocols using mhcii

Murine Transgenic Immune Cell Models

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CX₃CR1-STOP-DTR, CX₃CR1-DTR and CX₃CR1-CreERT2 were previously described (Diehl et al., 2013 (link); Longman et al., 2014 (link); Parkhurst et al., 2013 (link)). The following strains are from Jackson Laboratories: CD11c-Cre (JAX # 008068), MHCII^−/− (JAX # 003584), MHCII-conditional (JAX # 013181) and IL-10^−/− (JAX # 003968), VertX (JAX # 014530), CX₃CR1-GFP (JAX # 005582), RAG2^−/− (JAX # 008449), Ly5.1⁺ (JAX # 002014), OTII-Tg⁺ (JAX # 004194), and C57BL/6 mice (JAX # 000664). IL-10 and IL-10 receptor conditional mice were from Dr. Werner Muller (Pils et al., 2010 (link); Roers, 2004 (link)). All mice were kept in specific pathogen-free conditions and bred at the animal facility of the Skirball Institute or Baylor College of Medicine. Mouse experiments were performed with at least 3 mice per group and all mice were bred to generate littermate control animals for each experiment. Littermates were randomly assigned to experimental groups. Animals were used between 6 – 12 weeks of age with males and females used in approximately equal ratios. All animal experiments were performed in accordance with approved protocols for the NYU or BCM Institutional Animal Care and Usage Committee.

Kim M., Galan C., Hill A.A., Wu W.J., Fehlner-Peach H., Song H.W., Schady D., Bettini M.L., Simpson K.W., Longman R.S., Littman D.R, & Diehl G.E. (2018). Critical role for the microbiota in CX3CR1+ intestinal mononuclear phagocyte regulation of intestinal T cell responses. Immunity, 49(1), 151-163.e5.

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Genetically Modified Mouse Models

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Female 7–9 week old C57BL/6, IL-12p40^-/-, IL-12p35^-/-, MyD88^-/-, Batf3^-/-, IFN-γ^-/-, CD8^-/-, and MHCII^-/- mice were purchased from Jackson laboratories (Bar Harbor, ME) and were maintained under specific pathogen-free conditions at the Center for Comparative Medicine and Research at the Geisel School of Medicine at Dartmouth.

Fox B.A., Sanders K.L., Rommereim L.M., Guevara R.B, & Bzik D.J. (2016). Secretion of Rhoptry and Dense Granule Effector Proteins by Nonreplicating Toxoplasma gondii Uracil Auxotrophs Controls the Development of Antitumor Immunity. PLoS Genetics, 12(7), e1006189.

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Long-term Breeding of Genetically Modified Mice

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A long-term breeding colony of WT, Rag1^-/-, MhcII^-/-, IgA^-/- and AID^-/- mice (all C57BL/6 background) has been maintained by the Kubinak Lab at the University of South Carolina for four years. WT (Jax#000664), Rag1^-/- (Jax#002216), and MhcII^-/- (Jax#003584) mice were originally purchased from Jackson laboratories. All animals used in the experiments described here were derived from this colony. Male and female mice were used in all experiments. Mice were reared and maintained in a single environmentally controlled room exclusively used to house this mouse colony. Mice were maintained under constant environmental conditions (70°F, 50% relative humidity, 12:12 light:dark cycles) and were given ad libitum access to autoclaved drinking water and an irradiated soy-free mouse chow (Envigo; diet#2920X). All animal use strictly adhered to federal regulations and guidelines set forth by the University of South Carolina Institutional Animal Care and Use Committee (Protocol#101580).

Roland M.M., Peacock T.E., Hall N., Mohammed A.D., Ball R., Jolly A., Alexeev S., Dopkins N., Nagarkatti M., Nagarkatti P, & Kubinak J.L. (2023). B-cell-specific MhcII regulates microbiota composition in a primarily IgA-independent manner. Frontiers in Immunology, 14, 1253674.

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Genetically Modified Mouse Models

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Male (6wk) C57BL/6 mice were purchased from Taconic Biosciences (Hudson, NY). 4–1BB^−/−, EBI3^−/−, IL27 receptor alpha^−/−, β2M^−/−, MHCII^−/−, Foxp3-DTR, CXCR3^−/−, CCR2^−/−, and CCR5^−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME). All procedures were conducted in accordance with the guidelines established by the U.T. MD Anderson Cancer Center Institutional Animal Care and Use Committee.

Bartkowiak T., Jaiswal A.R., Ager C.R., Chin R., Chen C.H., Budhani P., Ai M., Reilley M.J., Sebastian M.M., Hong D.S, & Curran M.A. (2018). Activation of 4-1BB on liver myeloid cells triggers hepatitis via an interleukin-27 dependent pathway. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(5), 1138-1151.

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Comparative Analysis of Mouse Strains

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Wild-type, MHCII^−/−, and Rag1^−/− C57BL/6 mice (female, 5 to 6 weeks of age) were all purchased from Jackson Laboratories.

Zhang F., Thompson C., Ma N., Lu Y.J, & Malley R. (2022). Carrier Proteins Facilitate the Generation of Antipolysaccharide Immunity via Multiple Mechanisms. mBio, 13(3), e03790-21.

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Genetic Mouse Models for Immunology

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Male and female, 6–10 week-old on B6 genetic background mice were used in this study. C57BL/6J wild type, MHC-II^−/−, CXCR5^−/−, TCRβ^−/−, TCRγδ-GFP, and TCRδ^−/− mice were purchased from the Jackson Laboratory. Tcf7L (TCF-1) Tg mice were kindly provided by Dr. Ana Anderson from Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA. OT-II-Foxp3-GFP mice were housed in a conventional specific pathogen-free facility at the Harvard Institutes of Medicine and/or at the Building for Transformative Medicine according to the animal protocol with the full knowledge and permission of the Standing Committee on Animals at Harvard Medical School and Brigham and Women’s Hospital. Three to ten mice were used per group and every experiment was reproduced at least twice, which is commonly used for in vivo studies to alleviate unnecessary animal suffering. No animal was excluded from analysis and no randomization was performed.

Rezende R.M., Lanser A.J., Rubino S., Kuhn C., Skillin N., Moreira T.G., Liu S., Gabriely G., David B.A., Menezes G.B, & Weiner H.L. (2018). γδ T cells control humoral immune response by inducing T follicular helper cell differentiation. Nature Communications, 9, 3151.

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Genetically Modified Mouse Strains

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Six to eight-week-old C57BL/6 Ly5.1 (CD45.1), Ly5.2 (CD45.2), WT mice were purchased from Charles River or Jackson Laboratory. CCR2^-/-, IL-10^-/-, H–2K^bm1, OTI, OTII, MHCII^-/- and IL-10 GFP (Tiger) mice were purchased from Jackson Laboratory. PDL1/2^-/- mice were kindly provided by Dr. Daniel Barber and Keith Kauffman. OTII mice were crossed with IL-10 GFP to generate OTIl IL-10 GFP reporter mice. All mice were genotyped upon arrival and prior to their use in experiments. Mice were housed in a specific pathogen-free environment at National Jewish Health and Dartmouth Hitchcock Medical College, an AAALAC accredited institution, and used in accordance with protocols approved by the Institutional Animal Care and Utilization Committee.

Tewari A., Prabagar M.G., Gibbings S.L., Rawat K, & Jakubzick C.V. (2021). LN Monocytes Limit DC-Poly I:C Induced Cytotoxic T Cell Response via IL-10 and Induction of Suppressor CD4 T Cells. Frontiers in Immunology, 12, 763379.

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Genetically Modified Mouse Strains

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Male and female, 8-10 week-old and on a B6 genetic background mice were used in this study. C57BL/6 wild type, congenic CD45.1, RAG-1−/−, CCR6−/−, MHC-II−/− and β₇−/− mice were purchased from the Jackson Laboratory. CCR9−/− mice were kindly provided by Dr. Jesus Rivera-Nieves (University of California at San Diego – UCSD). ATF3−/−, Foxp3-GFP, OT-IIxFoxp3-GFP and 2D2xFoxp3-GFP mice were housed in a conventional specific pathogen-free facility at the Harvard Institutes of Medicine according to the animal protocol with the full knowledge and permission of the Standing Committee on Animals at Harvard Medical School.

Rezende R.M., da Cunha A.P., Kuhn C., Rubino S., M'Hamdi H., Gabriely G., Vandeventer T., Liu S., Cialic R., Pinheiro-Rosa N., Oliveira R.P., Gaublomme J.T., Obholzer N., Kozubek J., Pochet N., Faria A.M, & Weiner H.L. (2015). Identification and characterization of latency-associated peptide-expressing γδ T cells. Nature Communications, 6, 8726.

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Genetic Manipulation of Mouse Models

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Male and female, 8–10-week-old and on a B6 genetic background mice were used in this study. C57BL/6 wild type, congenic CD45.1, RAG-1−/−, CCR6−/−, MHC-II−/− and β₇−/− mice were purchased from the Jackson Laboratory. CCR9−/− mice were kindly provided by Dr Jesus Rivera-Nieves (University of California at San Diego—UCSD). ATF3−/−, Foxp3-GFP, OT-IIxFoxp3-GFP and 2D2xFoxp3-GFP mice were housed in a conventional specific pathogen-free facility at the Harvard Institutes of Medicine according to the animal protocol with the full knowledge and permission of the Standing Committee on Animals at Harvard Medical School.

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Murine Models for Immunology Research

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BALB/c (Strain #000651), C57BL/6 (Strain #000664), muMT (Strain #002288), B2m (Strain #002087), and MHCII (Strain #003584) mice of both sexes were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Mice were housed and bred in a specific pathogen-free environment at the University of Massachusetts Chan Medical School (UMCMS). All experimental protocols were approved by the UMCMS Institutional Use and Care of Animals Committee.

Hester M.M., Oliveira L.V., Wang R., Mou Z., Lourenco D., Ostroff G.R., Specht C.A, & Levitz S.M. (2022). Cross-reactivity between vaccine antigens from the chitin deacetylase protein family improves survival in a mouse model of cryptococcosis. Frontiers in Immunology, 13, 1015586.

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