MKN45‐Luc cells treated with si‐CXCR6 for 5 days were collected and fixed with 10% (v/v) formalin in PBS. Then cells were washed with PBS and incubated with mouse anti‐human CXCR6 antibody (R&D Systems) at a final concentration of 25 μg/mL, followed by incubation with Alexa488‐conjugated anti‐mouse IgG (Life Technologies, Carlsbad, CA, USA). Flow cytometric analysis was performed using the BD FACSVerse (Becton Dickinson, Franklin Lakes, NJ, USA).
Mouse anti human cxcr6 antibody
Manufactured by R&D Systems
Sourced in United States
Mouse anti-human CXCR6 antibody is a laboratory research reagent used to detect and study the CXCR6 protein, which is a chemokine receptor expressed on certain immune cells.
Automatically generated - may contain errors
2 protocols using mouse anti human cxcr6 antibody
1
CXCR6 Knockdown in MKN45-Luc Cells
2
Evaluating Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Migratory and invasive potential of PC3, LNCaP and RWPE-1 cells were evaluated using BD BioCoat migration or matrigel invasion chambers (BD Labware, NJ, USA) respectively, as described in previous studies [20 (link), 22 (link)]. Briefly, 2×104 cells were added to the top chamber of inserts and allowed to migrate under chemotactic gradient of recombinant human CXCL16 (100 ng/mL; PeproTech, NJ, USA) added to the bottom chamber as chemo-attractant. To verify that observed responses are CXCR6 dependent, cells were pre-incubated with 1.0 μg/mL mouse anti-human CXCR6 antibody (R&D Systems) for 1 h before the assay. The cells, with and without anti-CXCR6 antibody, were incubated for 16 h at 37°C in 5% CO2. Following incubation, cells were fixed with 100% methanol and stained with 1% toluidine blue supplemented with 1% borax. Stained cells were counted by microscope at 40X magnification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!