Hba1c

Manufactured by Bio-Rad

Sourced in United States

HbA1c is a laboratory instrument used for the measurement of glycated hemoglobin in blood samples. It provides an assessment of average blood glucose levels over a 2-3 month period.

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Lab products found in correlation

Cobas c701, by Roche (2 mentions) Variant 2 turbo, by Bio-Rad (1 mentions) Radioimmunoassay, by Merck Group (1 mentions)

5 protocols using hba1c

Anthropometric and Biochemical Assessment

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For the estimation of biochemical parameters viz. FBS (Fasting Blood Sugar, Rxl-Max 500), OGTT (Oral Glucose Tolerance Test), HbA1c (Bio-Rad D-10), Triglycerides, Cholesterol, HDL, LDL, Chol/HDL ratio, HDL/LDL ratio (Rxl-Max 500) and VLDL about 9 ml of blood was drawn and analyzed by phlebotomist of Sisco Research Laboratories (SRL) of Chandigarh. Anthropometric measurements were also obtained (i.e. height, weight, waist circumference) by trained researcher. The waist circumference (WC) was reported in centimeters. The BMI was obtained by using the formula (weight in kg/height (meter)²).

Kaur N., Majumdar V., Nagarathna R., Malik N., Anand A, & Nagendra H.R. (2021). Diabetic yoga protocol improves glycemic, anthropometric and lipid levels in high risk individuals for diabetes: a randomized controlled trial from Northern India. Diabetology & Metabolic Syndrome, 13, 149.

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Cardiovascular Risk Assessment Protocol

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Demographic information was collected by physicians. Height and weight were measured by electronic scales. Two seated blood pressure measurements were obtained by a well-trained nurse following a standard protocol using a mercury sphygmomanometer after the patients had rested quietly for at least 5 min, and the average of the two measurements was used for analysis. The patients fasted for at least 8 hours overnight, and blood and urine samples were collected the following morning. All blood and urine samples were tested immediately after collection. The tests included hemoglobin A1c (HbA1c) (Bio-Rad V ARIANTII TURBO, America), fasting blood glucose (FBG), serum UA, blood urea nitrogen (BUN), serum CR, triglyceride (TG), total cholesterol (TC), HDL-C, low-density lipoprotein cholesterol (LDL-C), and albumin excretion rate (AER) (Roche Cobas c 701, Germany).

Han R., Duan L., Zhang Y, & Jiang X. (2023). Serum Uric Acid is a Better Indicator of Kidney Impairment Than Serum Uric Acid-to-Creatinine Ratio and Serum Uric Acid-to-High-Density Lipoprotein Ratio: A Cross-Sectional Study of Type 2 Diabetes Mellitus Patients. Diabetes, Metabolic Syndrome and Obesity, 16, 2695-2703.

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Anthropometric and Metabolic Measurements

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Height and weight were measured without shoes and with light clothes. Body mass index (BMI) was calculated as weight (kg) divided by height (m) squared. Blood pressure was measured in the sitting position after 5 minutes of rest using a Mercury300 mercury phygmomanometer, and the mean of two values was used for systolic and for diastolic pressure. Systolic blood pressure was read at the first Korotkoff sound and diastolic blood pressure at the disappearance of the Korotkoff sounds (phase V). The deflation rate was 2 mmHg/second. Before the investigation, the manometer was calibrated. Fasting blood samples were analyzed for HbA1c (BioRad, USA). LDL cholesterol was measured using enzymatic colorimetric methods (Roche, Mannheim, Germany).

Friedrich N., Pietzner M., Cannet C., Thuesen B.H., Hansen T., Wallaschofski H., Grarup N., Skaaby T., Budde K., Pedersen O., Nauck M, & Linneberg A. (2017). Urinary metabolomics reveals glycemic and coffee associated signatures of thyroid function in two population-based cohorts. PLoS ONE, 12(3), e0173078.

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Comprehensive Metabolic Profiling in Diabetics

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Demographic information and medication history were collected by physicians. All the patients were given hypoglycemic drugs. Height and weight were measured by electronic scales. Two seated blood pressure measurements were obtained by a well-trained nurse using a mercury sphygmomanometer after the patients rested quietly for at least 5 min following a standard protocol, with the average of the two measurements used for the analysis. The patients fasted for at least 8 hours overnight, and blood and urine samples were collected the following morning. All blood and urine samples were tested immediately after collection. The tests included hemoglobin A1C (HbA1c) (Bio-Rad VARIANTII TURBO, America), fasting blood glucose (FBG), serum uric acid (SUA), blood urea nitrogen (BUN), creatinine (CR), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), and albumin excretion rate (AER) (Roche Cobas c 701, Germany).

Han R., Zhang Y, & Jiang X. (2022). Relationship Between Four Non-Insulin-Based Indexes of Insulin Resistance and Serum Uric Acid in Patients with Type 2 Diabetes: A Cross-Sectional Study. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 15, 1461-1471.

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Biochemical Analyses for Metabolic Markers

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Analyses were performed by the University of Colorado Anschutz Research core laboratory or the Children’s Hospital Colorado clinical laboratory except where noted. Triglycerides and HDL were analyzed enzymatically (Hitachi 917 autoanalyzer; Boehringer Mannheim Diagnostics, Indianapolis, IN). DCCT-calibrated high performance ion-exchange liquid chromatography was used for HbA1c (Bio-Rad Laboratories, Hercules, Calif). Radioimmunoassay was used to analyze serum insulin (Millipore, Billerica, MA). Melatonin was assayed using Radioimmunoassay (Buhlmann Laboratories AG, Schonenbuch, Switzerland) at the Sleep and Chronobiology Laboratory at the University of Colorado Boulder.

Simon S.L., Behn C.D., Cree-Green M., Kaar J.L., Pyle L., Hawkins S.M., Rahat H., Garcia-Reyes Y., Wright KP J.r, & Nadeau K.J. (2018). Too Late and Not Enough: School Year Sleep Duration, Timing, and Circadian Misalignment Are Associated with Reduced Insulin Sensitivity in Adolescents with Overweight/Obesity. The Journal of pediatrics, 205, 257-264.e1.

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