Fitc conjugated ki 67 antibody

Manufactured by BD

Sourced in United States

The FITC conjugated Ki-67 antibody is a laboratory reagent designed for the detection and quantification of the Ki-67 protein. Ki-67 is a nuclear protein expressed during active phases of the cell cycle, making it a useful marker for cell proliferation. The FITC (Fluorescein Isothiocyanate) conjugation allows for the visualization of the Ki-67 protein through fluorescence-based detection methods.

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Lab products found in correlation

Foxp3 staining buffer set, by Thermo Fisher Scientific (3 mentions) Facscalibur, by BD (2 mentions) α sma, by Boster Bio (1 mentions)

Spelling variants of this product

Ki-67 antibody (18 citations) FITC-Ki-67 (7 citations) Ki-67-FITC antibody (3 citations) Ki-67 (2 citations)

4 protocols using fitc conjugated ki 67 antibody

Ki-67 Expression Analysis in BEL-7404 Cells

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Detection of Ki-67 was done according to our previous study.¹⁶ In brief, BEL-7404 cells were treated with different concentrations (0,
200, 400, and 600 μg/mL) of CTPG or cisplatin (35 μg/mL). After 24 hours, cells
were harvested and washed with PBS, then fixed and permeabilized with Foxp3
Staining Buffer Set (eBioscience, USA) according to the manufacturer’s
instructions. Intracellular staining was performed using FITC conjugated
Ki-67 antibody (BD Biosciences, San Jose, CA, USA) for
15 minutes at RT. The samples were analyzed by flow cytometry (BD FACSCalibur,
CA, USA).

Yuan P., Fu C., Yang Y., Adila A., Zhou F., Wei X., Wang W., Lv J., Li Y., Xia L, & Li J. (2021). Cistanche tubulosa Phenylethanoid Glycosides Induce Apoptosis of Hepatocellular Carcinoma Cells by Mitochondria-Dependent and MAPK Pathways and Enhance Antitumor Effect through Combination with Cisplatin. Integrative Cancer Therapies, 20, 15347354211013085.

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Assessing Cell Proliferation in A549 Cells

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A549 cells were treated with different concentrations (200, 400 and 600 μg/mL) of BRBS. After 24 h, cells were harvested and washed with PBS. After washing, cells were fixed and permeabilized with Foxp3 Staining Buffer Set (eBioscience, San Diego, CA, USA) according to the manufacturer’s instructions. Intracellular staining was performed using FITC conjugated Ki-67 antibody (BD Biosciences, San Jose, CA, USA) for 15 min at RT. The samples were analyzed by flow cytometry.

Aipire A., Chen Q., Cai S., Li J., Fu C., Ying T., Lu J, & Li J. (2018). N-Butanol Subfraction of Brassica Rapa L. Promotes Reactive Oxygen Species Production and Induces Apoptosis of A549 Lung Adenocarcinoma Cells via Mitochondria-Dependent Pathway. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(7), 1687.

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Ki-67 Expression in BEL-7404 Cells

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Detection of Ki-67 was done according to our previous study [16] . In brie y, BEL-7404 cells were treated with different concentrations (0, 200, 400 and 600 μg/mL) of CTPG or cisplatin (35 μg/mL). After 24 h, cells were harvested and washed with PBS, then xed and permeabilized with Foxp3 Staining Buffer Set (eBioscience, USA) according to the manufacturer`s instructions. Intracellular staining was performed using FITC conjugated Ki-67 antibody (BD Biosciences, San Jose, CA, USA) for 15 min at RT. The samples were analyzed by ow cytometry (BD FACSCalibur, CA, USA).

Yuan P., Fu C., Yi Y., Aipire A., Zhou F., Wei X., Wang W., Lv J., Li Y., Xia L., & Li J. (2021). Cistanche Tubulosa Phenylethanoid Glycosides Induce Apoptosis of Hepatocellular Carcinoma Cells by Mitochondria-Dependent and MAPK Pathways and Enhance Antitumor Effect Through Combination With Cisplatin.

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Immunofluorescence Analysis of Liver Cells

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The paraffin-embedded liver tissue sections were dewaxed and rehydrated, and after the antigen retrieval, the sections were permeabilized with Triton X-100 and incubated with primary antibodies against α-SMA (Boster, Wuhan, China) and SEPT6 (Proteintech) at 4 °C overnight. Then, the tissues were washed, incubated with an Alexa Fluor 488conjugated anti-mouse antibody and Alexa Fluor 594 conjugated anti-rabbit antibody (Promoter Biotechnology, Wuhan, China). The nuclei were counterstained with DAPI (Promoter).
The paraffin-embedded liver tissue sections from the adenovirus-treated rats were incubated with a FITCconjugated Ki67 antibody (BD, BioScience, New Jersey, USA) and an anti-α-SMA (clone 1A4) cy3-labelled (Sigma-Aldrich, Hamburg, Germany) antibody to detect the proliferative rate of the HSCs. A FITC-conjugated TUNEL assay (Roche, Mannheim, Germany) was performed with anti-α-smooth muscle actin (SMA; clone 1A4) cy3-labelled antibody staining to detect the apoptotic rate in the HSCs as previously described [28] . The nuclei were counterstained with DAPI (Promoter). All immunofluorescence staining assays were performed according to the manufacturer's instructions. Digital images were taken under a fluorescence microscope. (×100 magnification)

Fan Y., Du Z., Steib C.J., Ding Q., Lu P., Tian D, & Liu M. (2019). Effect of SEPT6 on the biological behavior of hepatic stellate cells and liver fibrosis in rats and its mechanism. Laboratory investigation; a journal of technical methods and pathology, 99(1).

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