Spectra por 12 14 kda cut off dialysis tubing

Fura‐2/AM, pluronic acid F‐127 and fluorescein conjugated‐BSA were obtained from Thermo Fisher Scientific (Waltham, MA, USA); bromosulphophthalein was obtained from Fluka (Fluka, Buchs, Switzerland); and hydroxyethyl piperazineethanesulphonic acid (Hepes) and all other chemicals were obtained from Sigma‐Aldrich (St Louis, MO, USA).
Heat shock, fraction V BSA was from Roche (Basel , Switzerland). BSA was extensively dialysed against normal saline plus 10 mm Hepes using Spectra/Por 12–14 kDa cut‐off dialysis tubing (Thermo Fisher Scientific).

Hydroxyethyl piperazineethanesulfonic acid (HEPES) and all other chemicals were obtained from Sigma-Aldrich (St Louis, MO, USA) or VWR BDH chemicals (Radnor, PA, USA). Fura-2/AM, fluo-4/AM, calcein/AM, pluronic acid F-127, and fluorescein conjugated-BSA were obtained from Thermo Fisher Scientific (Waltham, MA, USA), bromosulfophthalein (BSP) was from Fluka (Fluka, Buchs, Switzerland), and 190 proof ethyl alcohol from Decon, Labs Inc. Heat shock, fraction V BSA was from Roche (Basel, Switzerland). BSA was extensively dialyzed against normal saline plus 10 mM HEPES using Spectra/Por 12–14 kDa cut-off dialysis tubing (Thermo Fisher Scientific).
Dr. K. Mikoshiba (RIKEN) generously provided the plasmid pIRIS-1 and pRatiometric-pericam-nu was a gift from A. Miyawaki (RIKEN). pGP-CMV-GCaMP6f was a gift from Douglas Kim & GENIE Project (Addgene plasmid # 40755; http://n2t.net/addgene:40755; RRID:Addgene_40755). Adenovirus particles expressing PKCβII-enhanced green fluorescent protein (Ad-PKCβII-EGFP) were a gift from Dr. R. Rizzuto (University of Padua).