KRC cells were cultured [36 (link)], confirmed as mycoplasma-free (Mycoalert kit; Lonza) and seeded in 6-well plates. After 24h, cells were serum-starved overnight, and incubated with control media or TGF-β1 ([0.5nM], 24h). Medium was collected, centrifuged, filtered through a 0.22 μm filter, and cytokine levels were determined using a Milliplex ELISA kit (Millipore).
Milliplex elisa kit
Manufactured by Merck Group
Sourced in United States
The Milliplex ELISA kit is a diagnostic tool used for the quantitative detection of specific proteins or other analytes in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) principle to perform multiplex analysis, allowing the simultaneous measurement of multiple targets in a single sample. The core function of the Milliplex ELISA kit is to provide a reliable and efficient method for conducting sensitive and quantitative protein or analyte measurements in a variety of research and clinical applications.
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Lab products found in correlation
3 protocols using milliplex elisa kit
1
TGF-β1 Stimulation of KRC Cells
2
Measuring Inflammatory Mediators in Mouse Serum
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The levels of TSLP and tryptase were measured using a mouse TSLP ELISA kit (catalog #MTLP00; R&D Systems, Minneapolis, MN, USA) and mouse tryptase ELISA kit (catalog #RD-TPS-Mu; Reddot Biotech Inc., Kelowna, BC, Canada), respectively. Additionally, the serum levels of mouse IL-13, IL-33, and TNF-α were detected using a MILLIPLEX ELISA kit (catalog #MCYTOMAG-70K; Millipore, Billerica, MA, USA).
3
Cytokine Profiling of TGF-β1 Treated Cells
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KRC cells were seeded in 6-well plates (200,000/well). After 24 h, cells were serum-starved overnight, and treated with control media or TGF-β1 [0.5 nM] for 24 h. CM was prepared, and levels of the indicated cytokines were determined using a Milliplex ELISA kit (Millipore) per the manufacturer's recommendation.
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