Dsirna nc1

Manufactured by Integrated DNA Technologies

DsiRNA NC1 is a laboratory reagent designed for RNA interference (RNAi) studies. It serves as a negative control for DsiRNA experiments, allowing researchers to assess the specificity of their RNAi effects. The product consists of a double-stranded RNA (dsRNA) molecule that does not target any known gene in the tested organism.

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Lab products found in correlation

Nucleofector kit t, by Lonza (1 mentions) Nucleofector technology, by Lonza (1 mentions) Pparα sirna, by Santa Cruz Biotechnology (1 mentions) X tremegene sirna transfection reagent, by Roche (1 mentions)

Close spelling variants of this product

DsiRNA

2 protocols using dsirna nc1

Silencing lncRNA in NALM-6 cells

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Custom Silencer Select siRNA (Ambion by Life Technologies) and Custom dsiRNA Duplex (Integrated DNA Technologies) were used to silence the lncRNA. Silencer Select negative control No.1 siRNA (Ambion) and dsiRNA NC1 (Integrated DNA Technologies) were used as negative control siRNA/dsiRNA. Lonza's Nucleofector™ Technology was used for the transfection of siRNA/dsiRNA into the NALM-6 cells using the Nucleofector kit T (Lonza) and the program C-005; silencing was measured 24 hours later by RT-qPCR.

Ouimet M., Drouin S., Lajoie M., Caron M., St-Onge P., Gioia R., Richer C, & Sinnett D. (2016). A childhood acute lymphoblastic leukemia-specific lncRNA implicated in prednisolone resistance, cell proliferation, and migration. Oncotarget, 8(5), 7477-7488.

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PPARα Silencing in MLO-A5 Cells

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PPARα silencing was achieved using Pparα siRNA (Santa Cruz Biotechnology), with DsiRNA NC1 (Integrated DNA Technologies, Coralville, IA) as a negative control. RNA oligonucleotides were delivered to MLO-A5 cells using the X-treme Gene siRNA Transfection Reagent (Roche; Cat#04476093001) according to a protocol provided by the manufacturer. αA5^KD cells and their scrambled (Scrl) control were used for assays 36 h after transfection.

Chougule A., Baroi S., Czernik P.J., Crowe E., Chang M.R., Griffin P.R, & Lecka-Czernik B. (2023). Osteocytes contribute via nuclear receptor PPAR-alpha to maintenance of bone and systemic energy metabolism. Frontiers in Endocrinology, 14, 1145467.

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