Ko143

MN (purity > 98%) and HK (purity > 98%) were purchased from ChemFaces (Wuhan, PRC). Ko143 (purity 96%) was obtained from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). MXR (purity 99%) was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Dimethyl sulfoxide (DMSO), formic acid, sodium dodecyl sulfate (SDS), 3-(4′,5′-dimethylthiazol-2′-yl)-2,5-diphenyltetrazolium bromide (MTT), butylparaben and triton X-100 were supplied by Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Biological Industries Inc. (Kibbutz, Beit Haemek, Israel). Penicillin-Streptomycin-Glutamine, Dulbecco’s modified Eagle medium (DMEM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Hank’s buffered salt solution (HBSS)and trypsin/EDTA were purchased from Invitrogen (Grand Island, NY, USA). Antibodies against ABCG2 and GAPDH were purchased from GeneTex (San Antonio, TX, USA). Polyvinylidene fluoride transfer membranes (Immobilon P) and chemiluminescence (ECL) were obtained from Millipore Corp. (Bedford, MA, USA). 4′,6-diamidino-2-phenylindole (DAPI) staining solution and goat anti-rabbit IgG H&L (DyLight™ 488) antibody were purchased from Abcam (Cambridge, UK). Acetonitrile (ACN) and methanol with LC grade were obtained from Mallinckrodt Baker (Phillipsburg, NJ, USA). Milli-Q plus water (Bedford, MA, USA) was used throughout this study.

(±)Warfarin sodium (purity 98%), caffeic acid (purity 98%), formic acid, 3-(40,50-dimethylthiazol-20-yl)-2,5-diphenyltetrazolium bromide (MTT) and sodium dodecyl sulfate were purchased from Sigma-Aldrich Chemical Co. (MO, U.S.A.). R-warfarin (purity 98%) and S-warfarin (purity 98%) were obtained from Toronto Research Chemicals, Inc. (Ontario, Canada). Ko143 (purity 96%) was obtained from Enzo Life Sciences, Inc. (NY, U.S.A) Fetal bovine serum, Dulbecco’s modified Eagle medium, penicillin–streptomycin-glutamine and Hank’s buffered salt solution (HBSS) were obtained from Invitrogen (CA, U.S.A). Resveratrol (purity > 99%) was obtained from TCI (Tokyo, Japan). Trans-resveratrol capsules were purchased from Doctor’s BEST (CA, U.S.A.). Acetonitrile and methanol of LC–MS grade were obtained from J.T. Baker Inc. (PA, U.S.A.).

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), bovine calf serum (BS), penicillin/streptomycin and trypsin 0.25% were purchased from Hyclone (GE Healthcare Life Sciences, Pittsburgh, PA, United States). 10X solution of phosphate buffered saline (PBS), SN-38, and Alexa Fluor 488 conjugated rabbit anti-mouse IgG secondary antibody were purchased from Thermo Fisher Scientific Inc. (Rockford, IL, United States). The monoclonal antibodies for ABCG2 (BXP-34), paclitaxel, vincristine, vinblastine, cisplatin, MX, verapamil, 3-(4, 5-dimethylthiazol-yl)-2, 5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), propidium iodide (PI), and Triton X-100, were obtained from Sigma Chemical Co. (St. Louis, MO, United States). Ko-143 and MK-571 were products from Enzo Life Sciences (Farmingdale, NY, United States). Monoclonal antibodies sc-47778 (against β-actin) and secondary HRP-labeled rabbit anti-mouse IgG were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, United States). [³H]- MX) (2.5 Ci/mmol) was purchased from Moravek Biochemicals (Brea, CA, United States). Liquid scintillation cocktail was a product from MP Biomedicals, Inc. (Santa Ana, CA, United States). All other chemicals were purchased from Sigma Chemical Co. (St. Louis, MO, United States).

Ko143 (a selective inhibitor of BCRP) was purchased from Enzo Life Sciences (Lausen, Switzerland). LY335979 (a selective inhibitor of P-gp) was a generous gift from Eli Lilly and Company. T0070907 (T007, specific inhibitor of PPARγ receptor) was purchased from Cayman Chemical Company (Ann Arbor, MI). Pioglitazone and rosiglitazone (internal standard, IS) were purchased from Cell Molecular Pharmaceutical R&D (Xi'an, China). All other reagents used were analytical grade.

PZQ, R(+)-verapamil HCl (dexverapamil), elacridar (GF120918, GG918), and MK 571 were from Sigma-Aldrich (St. Louis, MO). Tariquidar was from MedKoo Biosciences (Chapel Hill, NC). Ko143 was from Enzo Life Sciences (Farmingdale, NY), zosuquidar (LY335979) was from Toronto Research Chemicals (Toronto, ON), and Reversan was from Santa Cruz Biotechnology (Dallas, TX). Drugs were dissolved in dimethyl sulfoxide or ethanol for stock solutions, and were diluted to an appropriate concentration in culture media.

Topotecan (TPT) was purchased from Chemitek (Indianapolis, IN, United States). The mouse monoclonal anti-P-glycoprotein antibody, and 3-(4, 5-dimethylthiazol-yl)-2, 5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO), were supplied from MilliporeSigma Co. (St. Louis, MO, United States). Dulbecco’s modified Eagle’s medium (DMEM), 0.25% trypsin, and fetal bovine serum (FBS) were purchased from Corning Inc. (New York, NY, United States). The rabbit monoclonal antibodies against human MRP1/ABCC1 and human DNA topoisomerase I, the HRP-labeled goat anti-rabbit secondary antibody, and the HRP-linked rabbit anti-mouse secondary antibody were supplied from Cell Signaling (Danvers, MA, United States). Ko143, mitoxantrone (MX), cisplatin, and geneticin (G418) were obtained from Enzo life Sciences (Farmingdale, NY, United States). The mouse monoclonal antibodies for ABCG2 and glyceraldehyde phosphate dehydrogenase (GAPDH), the Alexa Fluor 488-labeled rabbit anti-mouse secondary antibody, 4,6-diamidino-2-phenylindole (DAPI), and all other chemicals were obtained from Thermo Fisher Scientific Inc. (Rockford, IL, United States).

M3814 was kindly provided by ChemieTek (Indianapolis, IN, USA). FBS, penicillin/streptomycin, DMEM, and trypsin EDTA were purchased from Corning Incorporated (Corning, NY, USA). mitoxantrone, [³H] (2.5 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA, USA). The Alexa Fluor 488 conjugated IgG secondary antibody and GAPDH monoclonal antibody (GA1R) (catalog number MA5-15738) were obtained from Thermo Fisher Scientific Inc (Rockford, IL, USA). Primary antibody against ABCG2, clone BXP-21 (catalog number MAB4146), was obtained from Millipore (Billerica, MA, USA). Anti-mouse IgG, HRP-linked antibody (catalog number 7076S) was purchased from Cell Signaling Technology Inc (Danvers, MA, USA). PBS and BSA were purchased from VWR chemicals, LLC (Solon, OH, USA). Ko143 was purchased from Enzo Life Sciences (Farmingdale, NY, USA). Paraformaldehyde, triton X-100, 4',6-diamidino-2-phenylindole (DAPI), mitoxantrone, doxorubicin, vincristine, verapamil, cisplatin, methylthiazolyldiphenyl-tetrazolium bromide (MTT), DMSO, and all other chemicals were requested from Sigma Chemical Co (St. Louis, MO, USA).