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2 protocols using disodium hydrogen phosphate

1

Antimicrobial Compounds Characterization

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Arbutin (≥98%), hydroquinone (ReagentPlus),
deuterated dimethyl sulfoxide (DMSO-d6), glycerol (99%), and sea salts were bought from Sigma-Aldrich.
Ethanol (100% undenatured), disodium hydrogen phosphate, sodium dihydrogen
phosphate, sodium hypochlorite solution (8–12.5%), and potassium
chloride were bought from Chem-Supply. KCl was purified by calcination
(8 h at 550 °C), recrystallization, and a second calcination
(8 h at 550 °C). Soybean peroxidase was bought from Bio-Research
Products Inc. Columbia horse
blood agar plates and TSB were acquired from Oxoid. Artificial seawater
was prepared from the sea salts from Sigma-Aldrich (38 g/L). Deionized
water was used for the bacterial testing and Milli-Q water for all
other experiments.
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2

Quantification of Flucloxacillin in Plasma

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Flucloxacillin sodium (FLX, 95 % purity) and 13C4-FLX sodium (SIL-IS, 99.4 % isotopic purity) were purchased from Toronto Research Chemicals (PM Separations, Australia). Acetonitrile (LC-MS grade) and water were purchased from Fisher Scientific (Scoresby, VIC, Australia). Water was further purified using a Simplicity UV purification system from Millipore Australia (North Ryde, NSW, Australia). Formic acid (LC-MS grade) was purchased from Fluka (Sigma- Aldrich, Australia). Disodium hydrogen phosphate was obtained from Chem-Supply (Gillman, SA, Australia). Expired human plasma (K3-EDTA) was obtained from the blood bank (SydPath, St Vincent’s Hospital, Sydney).
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