Fitc annexin 5 detection kit 1

Manufactured by BD

Sourced in United States

The FITC Annexin V Detection Kit is a laboratory reagent used to detect and quantify apoptotic cells. It contains Annexin V, a protein that binds to phosphatidylserine, and fluorescein isothiocyanate (FITC) for fluorescent labeling. The kit provides a tool for researchers to analyze and study cellular apoptosis processes.

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Lab products found in correlation

Cellquest software, by BD (2 mentions) Canto 2, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Fitc conjugated annexin 5, by BD (1 mentions) Facsverse system, by BD (1 mentions) Cyan adp cytofluorometer, by Beckman Coulter (1 mentions) Accuri c6 plus flow cytometer, by BD (1 mentions) Facsaria 3, by BD (1 mentions) Facsort flow cytometer, by BD (1 mentions) Lsrfortessa x 20 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

FITC-annexin V apoptosis detection Kit I (RUO) Annexin V–fluorescein isothiocyanate (FITC)-labeled Apoptosis Detection Kit I Annexin V fluorescein (FITC) Apoptosis Detection kit I Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit I FITC-Annexin V/propidium iodide (PI) Apoptosis Detection Kit I FITC Annexin V: PE Apoptosis Detection Kit I Annexin V-coupled fluorescein isothiocyanate (FITC) apoptosis detection kit-1 FITC-Annexin V/PI Detection Kit I Annexin V-FITC Apoptosis Detection assay kit I Fluorescein isothiocyanate-conjugated (FITC) anti-human Annexin V Apoptosis Detection Kit I

16 protocols using fitc annexin 5 detection kit 1

Apoptosis Detection in Hematopoietic Stem Cells

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BD Annexin V: FITC Detection kit I (BD Pharmingen) was used according to the
manufacturer’s instructions. Briefly, lineage-depleted BM cells were stained with
HSC-defining antibodies before washed and incubated with camptothecin at a final
concentration of 5 μM for 5 hours at 37°C. The samples were then washed with
ice-cold FACS buffer and resuspended in 1X Annexin binding buffer, to which Annexin V and
PI were added. The samples were vortexed and incubated in the dark for 15 minutes at room
temperature. Additional Annexin binding buffer was then added after which the samples were
analyzed within one hour.

Rundberg Nilsson A., Xian H., Shalapour S., Cammenga J, & Karin M. (2023). IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenance. bioRxiv.

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Detecting Apoptosis in Hematopoietic Stem Cells

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BD Annexin V: FITC Detection Kit I (BD Pharmingen) was used according to the manufacturer’s instructions. Briefly, lineage-depleted BM cells were stained with HSC-defining antibodies before being washed and incubated with camptothecin at a final concentration of 5 μM for 5 hours at 37°C. The samples were then washed with ice-cold FACS buffer and resuspended in 1× annexin binding buffer to which annexin V and propidium iodide were added. The samples were vortexed and incubated in the dark for 15 min at room temperature. Additional annexin binding buffer was then added after which the samples were analyzed within 1 hour.

Rundberg Nilsson A.J., Xian H., Shalapour S., Cammenga J, & Karin M. (2023). IRF1 regulates self-renewal and stress responsiveness to support hematopoietic stem cell maintenance. Science Advances, 9(43), eadg5391.

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Detecting Apoptosis in Hematopoietic Stem Cells

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Apoptosis Induction by ONC201

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Cells were incubated at 5 × 10⁵ cells/mL with (1.25, 2.5, 5.0, and 10 μM) or without ONC201 treatment for 48, 72 or 96 hrs, and then harvested. The apoptotic cells were analyzed by flow cytometry using the Annexin V-FITC Detection Kit I (BD Pharmingen, San Diego, CA) as previously described [33 (link)].

Ni X., Zhang X., Hu C.H., Langridge T., Tarapore R.S., Allen J.E., Oster W, & Duvic M. (2017). ONC201 selectively induces apoptosis in cutaneous T-cell lymphoma cells via activating pro-apoptotic integrated stress response and inactivating JAK/STAT and NF-κB pathways. Oncotarget, 8(37), 61761-61776.

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Afatinib and Radiation-Induced Apoptosis

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5-8F cells and HNE2 cells were pretreated for half an hour with vehicle or 2 μM afatinib before irradiation (4Gy) and were incubated for 48 hours. Next, the apoptotic cells were analysed via flow cytometry using the Annexin V-FITC Detection Kit I (BD Pharmingen, San Diego, CA) according to the manufacturer's instructions. Flow cytometry data were plotted and analysed via the fluorescence activated cell-sorting (FACS-Vantage) system using the Cell quest software (Becton-Dickinson, San Jose, CA) within 1 hour of staining. The percentages of differently labelled cells were calculated using the FlowJo software (Tree Star).

Huang F., Liang X., Min X., Zhang Y., Wang G., Peng Z., Peng F., Li M., Chen L, & Chen Y. (2019). Simultaneous Inhibition of EGFR and HER2 via Afatinib Augments the Radiosensitivity of Nasopharyngeal Carcinoma Cells. Journal of Cancer, 10(9), 2063-2073.

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Cell Proliferation and Apoptosis Assay

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For the proliferation assay, 20.000 cells were plated in triplicate in 24-well plates and cultured at 37°C. At the indicated time point the cells were collected and counted by trypan blue exclusion. The Annexin V-PI assay (apoptosis assay) was performed according to the manufacturer's instructions using the Annexin V-FITC detection kit I (BD Biosciences). Cell pellets were resuspended in 100 μl of binding buffer and incubated with 5 μl of FITC-conjugated Annexin-V and 5 μl of PI for 15 min at room temperature in the dark. 400 μl of 1 × binding buffer was added and the samples were immediately analyzed by flow cytometry. Samples were run on a Canto II flow cytometer (BD) and analyzed by FlowJo software, gating on live cells.

Moresco M.A., Raccosta L., Corna G., Maggioni D., Soncini M., Bicciato S., Doglioni C, & Russo V. (2018). Enzymatic Inactivation of Oxysterols in Breast Tumor Cells Constraints Metastasis Formation by Reprogramming the Metastatic Lung Microenvironment. Frontiers in Immunology, 9, 2251.

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Apoptosis Detection by Annexin V-FITC

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The Annexin V-FITC binding assay was performed using Annexin V-FITC detection kit I (BD Biosciences), according to the manufacturer's instructions. The cells were treated with ACT-3 and SAHA for 48 h. Cells were counted after trypsinization and washed twice with cold PBS. The cell pellet was resuspended in 100 µl binding buffer at a density of 1×10³ cells/ml and incubated with 5 µl FITC-conjugated Annexin-V and 5 µl PI for 15 min at room temperature in the dark. A total of 400 µl of 1X binding buffer were added to each sample tube, and the samples were immediately analyzed via flow cytometry (Accuri Cytometers, Inc.).

Lim J.S., Kyung S.Y., Jeon Y., Kim I.S., Kwak J.H, & Kim H.S. (2023). Anticancer effects of the HDAC inhibitor, 3β,6β‑dihydroxyurs‑12‑en‑27‑oic acid, in MCF‑7 breast cancer cells via the inhibition of Akt/mTOR pathways. Oncology Reports, 49(2), 43.

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Annexin V-FITC Apoptosis Assay

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Annexin V-FITC Detection Kit I (BD Bioscience) was used to identify the distribution of early or late apoptosis induced by PBEA towards HeLa cells. The assay was conducted as instructed in manufacture's manual. The cells were later incubated with the IC 50 concentration of PBEA for 24 h, 48 h, and 72 h at 37 C with 5% CO 2. After that, the cells were washed two times with cold PBS and stained with 5 ml of FITC Annexin V and 5 ml of propium iodide (PI) for 15 min at room temperature under dark condition. Results for the stained cells were obtained (10,000 events per sample) through FACSCANTO II (BD Bioscience). The data acquired were analyzed by using FlowJo_V10 software. The test was repeated thrice independently.

Mohd-Salleh S.F., Wan-Ibrahim W.S, & Ismail N. (2020). Pereskia bleo Leaves Extract Induces Cell Death via Cell Cycle Arrest and Apoptosis in Cervical Cancer Cells HeLa. Nutrition and cancer, 72(5).

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Annexin V-FITC Apoptosis Assay

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Cell death was assessed by means of the FITC-AnnexinV Detection Kit I (BD Biosciences), as per standardized procedures.⁵¹ (link) Briefly, 10⁵ cells were collected per sample, washed in PBS, and resuspended in 1X binding buffer (10 mM HEPES/NaOH - pH 7.4, 140 mM NaCl, and 5 mM CaCl₂) containing fluorescein isothiocyanate (FITC)-conjugated AnnexinV (BD Biosciences) and 0.5 μg/mL PI, following the manufacturer’s instructions. Cytofluorometric determinations were performed on a FACSCalibur cytofluorometer equipped with a 70 μm nozzle. First line statistical analyses were performed by using the CellQuest™ software (BD Biosciences), by gating on the events characterized by normal forward and side scatter.

Sukkurwala A.Q., Adjemian S., Senovilla L., Michaud M., Spaggiari S., Vacchelli E., Baracco E.E., Galluzzi L., Zitvogel L., Kepp O, & Kroemer G. (2014). Screening of novel immunogenic cell death inducers within the NCI Mechanistic Diversity Set. Oncoimmunology, 3, e28473.

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Quantifying Apoptosis by Flow Cytometry

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The FITC Annexin V Detection Kit I (BD Pharmingen, San Diego, CA) was used to quantify apoptosis by flow cytometry according to the manufacturer’s instructions as previously described (13 (link)).

Fan P., Cunliffe H.E., Maximov P.Y., Agboke F.A., McDaniel R.E., Zou X., Ramos P., Russell M.L, & Jordan V.C. (2015). Integration of Downstream Signals of Insulin-like Growth Factor-1 Receptor by Endoplasmic Reticulum Stress for Estrogen-induced Growth or Apoptosis in Breast Cancer Cells. Molecular cancer research : MCR, 13(10), 1367-1376.

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