Accu check glucose meter

Manufactured by Roche

Sourced in United States

The Accu-Chek glucose meter is a portable device used to measure blood glucose levels. It provides a simple and convenient way for individuals to monitor their blood sugar levels.

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Lab products found in correlation

Streptozotocin (stz), by Merck Group (2 mentions) Novolin r, by Novo Nordisk (1 mentions) Corticosterone, by Enzo Life Sciences (1 mentions) Humulin, by Eli Lilly (1 mentions) Mcp 1 elisa kit, by R&D Systems (1 mentions) Cholesterol liquicolor, by EKF Diagnostics (1 mentions) Half micro test kit, by Roche (1 mentions) Oxiselect tbars assay kit, by Cell Biolabs (1 mentions) Alloxan, by Merck Group (1 mentions) Tween 20, by Merck Group (1 mentions) Mvx10, by Olympus (1 mentions) Ix71 inverted microscope, by Olympus (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Liaison xl platform, by DiaSorin (1 mentions) Cobas integrated platform, by Roche (1 mentions) Elisa, by Merck Group (1 mentions) Bms603 2, by Thermo Fisher Scientific (1 mentions) Bms607 3, by Thermo Fisher Scientific (1 mentions) Insulin lantus, by Sanofi (1 mentions)

18 protocols using accu check glucose meter

Glucose and Insulin Tolerance Tests

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Glucose tolerance tests (GTTs) were performed after the acclimatization period, at 3,
4, 8, and 12 weeks of dietary treatment, and after exercise training by the 16th
week. After an overnight fast, unanesthetized rats were injected intraperitoneally
(ip) with 50% glucose solution, 1.5 g/kg body weight (BW). Blood samples were
obtained from the tail vein before and at 30, 60, 90, and 120 min after glucose
injection. Insulin tolerance tests (ITTs) were performed after the acclimatization
period and at 8 and 12 weeks of dietary treatment. After an overnight fast,
unanesthetized rats were injected ip with Novolin R human insulin
(Novo Nordisk, Brazil), 0.75 U/kg BW. Blood samples were obtained from the tail vein
before and at 15, 30, 60, and 90 min after the insulin challenge. Blood glucose
concentrations were measured with an Accu-Check glucose meter (Roche Diagnostic,
USA).

Silva R.N., Bueno P.G., Avó L.R., Nonaka K.O., Selistre-Araújo H.S, & Leal A.M. (2014). Effect of physical training on liver expression of activin A and follistatin in a nonalcoholic fatty liver disease model in rats. Brazilian Journal of Medical and Biological Research, 47(9), 746-752.

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Diabetic Wound Healing with hBMSC-TERT

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Male Wistar rats were provided by the University of São Paulo (Ribeirão Preto, SP, Brazil) and maintained in individual polypropylene cages under controlled temperature, humidity, and lighting (12 h light/dark cycle), with food and water supplied ad libitum. The study was approved by the Animal Ethics Committee of Federal University of São Carlos (CEUA 97150416) and was carried out in agreement with the Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources, National Academy of Sciences, USA) and the principles of the Brazilian College of Animal Experimentation.
After 7 days of acclimation, animals (225±25 g) were randomly assigned to 4 groups: control rats wounded (CW, n=8), non-diabetic rats wounded and infused with hBMSC-TERT (C-TERT, n=10), diabetic rats wounded (DMW, n=8), and diabetic rats wounded and infused with hBMSC-TERT (DM-TERT, n=8). The diabetic rats received streptozotocin (STZ) (60 mg/kg, ip; Sigma-Aldrich, USA) and the non-diabetic rats received vehicle (sodium citrate, ip). Diabetes was checked 7 days after STZ injection and confirmed when rats were hyperglycemic (blood glucose >250 mg/dL) (23 (link)). Body mass and fasting glycemia were determined weekly. Blood samples were collected from the tail vein and glycemia was measured using an Accu-Check glucose meter (Roche Diagnostic, USA).

Luna G.L., Oehlmeyer T.L., Brandão G., Brassolatti P., Tosta J., Goto L.S., de Avó L, & Leal A.M. (2021). Use of human bone marrow mesenchymal stem cells immortalized by the expression of telomerase in wound healing in diabetic rats. Brazilian Journal of Medical and Biological Research, 54(11), e11352.

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Glucose, Insulin, and Lipid Measurements

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Blood glucose concentration was measured using an Accu-Check glucose meter (Roche Diagnostics, Edo. Mexico, Mexico). The hormone content of the basal samples was assessed in duplicate using commercially available ELISA kits for insulin (Crystal Chem, Downers Grove, IL, USA; sensitivity, 0.156 ng/mL; intra-assay coefficient of variability, 5.8%) and corticosterone (Enzo Life Sciences, Farmingdale, NY, USA; sensitivity, 26.99 pg/mL; intra-assay coefficient of variability, 4.8%). Colorimetric assays were performed for triglycerides and low-/high-density lipoprotein cholesterol (Randox, London, UK). The quantitative insulin sensitivity check index (QUICKY) was calculated according to the formula 1/(log(insulin) + log(glucose)) [33 (link)]. To convert insulin from ng/mL to pmol/L, we multiplied by 150 [36 ].

Vargas J., Junco M., Gomez C, & Lajud N. (2016). Early Life Stress Increases Metabolic Risk, HPA Axis Reactivity, and Depressive-Like Behavior When Combined with Postweaning Social Isolation in Rats. PLoS ONE, 11(9), e0162665.

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Glucose and Insulin Tolerance Tests

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After 16 h fasting, GTT was performed by orally administering 2.0 g glucose/kg body weight. Blood samples were taken from the tail vein to measure the glucose levels before and 15, 30, 60, 90 and 120 min after glucose administration. The ITT was conducted after 6 h fasting followed by an intra-peritoneal injection of 0.75 U/kg body weight Humulin (Eli Lilly). Blood glucose was measured by ACCU-Check glucose meter (Roche Diagnostics, Laval, QC, Canada).

Lee S.M., Dorotea D., Jung I., Nakabayashi T., Miyata T, & Ha H. (2017). TM5441, a plasminogen activator inhibitor-1 inhibitor, protects against high fat diet-induced non-alcoholic fatty liver disease. Oncotarget, 8(52), 89746-89760.

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Glucose and Insulin Tolerance Tests

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At week 10, animals were deprived of food for 12 h and OGTT was performed after gavage with 2 g of glucose per kilogram body weight in sterile phosphate-buffered saline and blood glucose levels were measured with an Accu-Check Glucose Meter (Roche Diagnostic, Milan, Italy) at 0, 15, 30, 60, and 120 min (n = 8). At the end of week 11, mice were deprived of food for 6 h and ITT was performed after intraperitoneal injection of 0.5 U insulin per kilogram body weight and blood glucose concentrations were detected at 0, 15, 30, 60, and 120 min (n = 8).

Liang Y., Zhan J., Liu D., Luo M., Han J., Liu X., Liu C., Cheng Z., Zhou Z, & Wang P. (2019). Organophosphorus pesticide chlorpyrifos intake promotes obesity and insulin resistance through impacting gut and gut microbiota. Microbiome, 7, 19.

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Fasting Blood Glucose Measurement in Rats

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After 12 h fasting, blood glucose from the rats in both groups was measured using an ACCU-Check glucose meter (Roche Diagnostics, Indianapolis, IN, USA).

Yu Y.W., Hsueh S.C., Lai J.H., Chen Y.H., Kang S.J., Chen K.Y., Hsieh T.H., Hoffer B.J., Li Y., Greig N.H, & Chiang Y.H. (2018). Glucose-Dependent Insulinotropic Polypeptide Mitigates 6-OHDA-Induced Behavioral Impairments in Parkinsonian Rats. International Journal of Molecular Sciences, 19(4), 1153.

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Glucose and Pyruvate Tolerance Tests in Mice

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OGTT, ITT, and pyruvate tolerance test (PTT) were performed during the last week of the intervention. After overnight fasting, mice were orally gavaged with 66% glucose solution (OGTT, 3 g/kg BW) or injected i.p. with sodium pyruvate (PTT, 2 g/kg BW). For ITT, mice were injected i.p. with insulin (0.75 U/kg BW) after fasting for 6 h. Blood glucose levels in the blood samples from tail veins were measured immediately before (t = 0 min) and at selected time points using an Accu-Check glucose meter (Roche).

Zhuang P., Li H., Jia W., Shou Q., Zhu Y., Mao L., Wang W., Wu F., Chen X., Wan X., Wu Y., Liu X., Li Y., Zhu F., He L., Chen J., Zhang Y, & Jiao J. (2021). Eicosapentaenoic and docosahexaenoic acids attenuate hyperglycemia through the microbiome-gut-organs axis in db/db mice. Microbiome, 9, 185.

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Biochemical Markers in Metabolic Profiling

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Blood glucose levels were determined using a glucometer (ACCU‐Check glucose meter, Roche, MO). Serum cholesterol and FFA levels were measured using Cholesterol Liquicolor (Catalog #1010, STANBIO Laboratory) and Half Micro Test Kit (Catalog #11 383 175 001, Roche), respectively. Serum MDA level was determined using OxiSelect TBARS Assay Kit (Catalog #STA‐330, CELL BIOLABS, CA). Serum Mcp‐1 protein level was measured by a Mcp‐1 ELISA kit (Catalog #DY479‐05, R&D, MN). Serum levels of ALT and AST were measured using ALT/GPT Liqui‐UV kit (Catalog #2930/430, STANBIO Laboratory) and AST/SGOT Liqui‐UV kit (Catalog #2930/2920, STANBIO Laboratory), respectively.

Lin H., Wang L., Liu Z., Long K., Kong M., Ye D., Chen X., Wang K., Wu K.K., Fan M., Song E., Wang C., Hoo R.L., Hui X., Hallenborg P., Piao H., Xu A, & Cheng K.K. (2022). Hepatic MDM2 Causes Metabolic Associated Fatty Liver Disease by Blocking Triglyceride‐VLDL Secretion via ApoB Degradation. Advanced Science, 9(20), 2200742.

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Metabolic Profile Assessment in Mice

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Glucose was measured on whole blood collected by tail bleed after a four hour fast at 8, 16, and 26 weeks using an Accu-Check glucose meter (Roche Diagnostics, Indianapolis, IN). Blood was collected at sacrifice and was used to measure alanine aminotransferase (ALT) using a Discret Pak ALT Reagent Kit (Catachem, Bridgeport, CT). To measure liver triglycerides, wet liver tissue was weighed and placed in a relative amount of Tris-Ethylenediaminetetraacetic acid-homogenization buffer solution to make a 100 mg/mL concentration and the tissue was homogenized to extract triglyceride and protein. An enzymatic assay was performed using Triglycerides Reagent Set (Pointe Scientific, Inc., Canton, MI). Photometric absorbance was read at 500 nm.

Bramlage K.S., Bhattacharjee J., Kirby M., Myronovych A., Gupta R., Gonzalez R.M., Xanthakos S., Bove K, & Kohli R. (2021). A Diet High in Fat and Fructose Induces Early Hepatic Mitochondrial Aging. Journal of pediatric gastroenterology and nutrition, 73(1), 99-102.

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Transgenic Mice Production Protocol

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To produce transgenic mice, micromanipulator system installed on an IX71 inverted microscope (Olympus, Tokyo, Japan) and FemtoJet Eppendorf microinjector (Hamburg, Germany) were used. HindIII and MfeI restriction enzymes were purchased from NEB corporation (Beverly, MA, USA). Immunostaining reagents; phosphate-buffered saline (PBS), paraformaldehyde (PFA), Sodium Dodecyl Sulfate (SDS), and Tween 20 were purchased from Sigma (Poole, UK). Alloxan was obtained from Sigma (Poole, UK). HCl and NaCl were obtained from Merck (Darmstadt, Germany). To detect blood glucose, Accu-check glucose meter from Roche Diagnostics (Basel, Switzerland) was used. Olympus MVX10 (Olympus Corp, Tokyo, Japan) was used for microscopy. Antibodies were purchased from abcam (Cambridge, UK) and Invitrogen (Grand Island, NY, USA).

Karami F., Asgari Abibeiglou B., Pahlavanneshan S., Farrokhi A., Tamadon A., Basiri M., Khalooghi K., Fallahi M, & Tahamtani Y. (2022). Enhanced characterization of beta cell mass in a Tg(Pdx1-GFP) mouse model. BioImpacts : BI, 12(5), 463-470.

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