Bs 23003r

Manufactured by Bioss Antibodies

Bs-23003R; is a lab equipment product manufactured by Bioss Antibodies. It is a piece of laboratory equipment designed for use in scientific research and analysis.

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Lab products found in correlation

2 protocols using bs 23003r

Quantification of Signaling Pathway Proteins

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MSCs or ECs were lysed using ice-cold lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Roche Applied Science, Penzberg, Germany). Proteins were fractionated by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis and then transferred to polyvinylidene difluoride (PVDF) membranes. Protein expression was detected by incubation with antibodies against CXCR4 (D1S7W; Cell Signaling Technology), phospho-VEGFR2 (bs-2674R; Bioss), integrin αvβ3 (bs-1310R; Bioss), p-FAK (bsm-52155R; Bioss), FAK (bs-20735R; Bioss), p-JAK2 (SY24-03; Novus), JAK2 (bs-23003R; Bioss), p-STAT3 (bs-1658R; Bioss), STAT3 (bsm-33218 M; Bioss), ATF3 (bs-0519R; Bioss), CHOP (bs-20669R; Bioss), p-ERK1/2 (D13.14.4E; Cell Signaling Technology), ERK1/2 (137F5; Cell Signaling Technology) or β-actin (AF0003; Beyotime).

Tang Y., Luo K., Chen Y., Chen Y., Zhou R., Chen C., Tan J., Deng M., Dai Q., Yu X., Liu J., Zhang C., Wu W., Xu J., Dong S, & Luo F. (2021). Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioactive Materials, 6(7), 2039-2057.

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Immunohistochemical analysis of JAK2/STAT3

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The paraffin retinal sections were processed according to IHC protocol and previous study (17 (link)). Photos were taken under an optical microscope (OLYMPUS, CX-21, Japan). Primary antibodies were as follows: JAK2 (bs-23003r, Bioss), STAT3 (60199-1-lg, Proteintech), p-JAK2 (ab32101, Abcam), p-STAT3 (sc-8059, Santa), Bcl-2 (ab194583, Abcam), and Bax (A19684, ABclonal). HRP-conjugated secondary antibodies were AS-1107 and AS-11069 (Aspen).

Wang S., Yu A., Han M., Chen X., Li Z., Ke M., Cai X., Ai M, & Xing Y. (2022). Pathological Changes and Expression of JAK-STAT Signaling Pathway Hallmark Proteins in Rat Retinas at Different Time Points After Retinal Ischemia Reperfusion Injury. Pathology and Oncology Research, 28, 1610385.

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