Infections employed H. polygyrus and N. brasiliensis, both maintained as previously described (Camberis et al., 2003 (link)). HpES was collected as previously described (Johnston et al., 2015 (link)). For succinate experiments, sodium succinate dibasic hexahydrate (Sigma-Aldrich) was dissolved in autoclaved tap water to 100 mM, filter-sterilized, and given to mice as their drinking water. To administer HpES in vivo, 5 µg in 100 µl PBS was given i.p. daily from day −1 to day +4 relative to addition of succinate to drinking water.
Sodium succinate dibasic hexahydrate
Sodium succinate dibasic hexahydrate is a chemical compound with the formula Na2(C4H4O4)·6H2O. It is a colorless crystalline solid that is soluble in water. The compound is used as a laboratory reagent and in various industrial applications.
Lab products found in correlation
7 protocols using sodium succinate dibasic hexahydrate
Helminth infection and succinate effects
Infections employed H. polygyrus and N. brasiliensis, both maintained as previously described (Camberis et al., 2003 (link)). HpES was collected as previously described (Johnston et al., 2015 (link)). For succinate experiments, sodium succinate dibasic hexahydrate (Sigma-Aldrich) was dissolved in autoclaved tap water to 100 mM, filter-sterilized, and given to mice as their drinking water. To administer HpES in vivo, 5 µg in 100 µl PBS was given i.p. daily from day −1 to day +4 relative to addition of succinate to drinking water.
High-Resolution Respirometry SUIT Protocol
A. baumannii Growth in Minimal Media
Cytotoxicity Evaluation of DOX in DMEM
Metabolite supplementation experiments
Hypoxia and Succinate Effects on hPDLCs
Modulating Tumor Cell Responses
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