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Gapdh mab

Manufactured by Abcam
Sourced in United States

GAPDH mAb is a mouse monoclonal antibody that recognizes the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a key enzyme involved in glycolysis and is widely used as a loading control in Western blotting and other protein analysis techniques.

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2 protocols using gapdh mab

1

HGF and c-MET Inhibitor Signaling Pathway Analysis

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Human recombinant HGF was purchased from GenScript (Nanjing, China). The c-MET kinase inhibitors JNJ38877605 and rapamycin (RAPA) were obtained from Selleckchem (Houston, TX). Antibodies used include c-MET mAb, p-MET (Tyr1234/1235) mAb, Notch mAb, HES-1 mAb, and GAPDH mAb. Antibodies used in this study include c-MET mAb, p-MET (Tyr1234/1235) mAb (Abcam, U.S.A.), Notch mAb (Abcam, U.S.A.), HES-1 mAb (Abcam, U.S.A.), GAPDH mAb (Abcam, U.S.A.), Anti-mouse IgG (Abcam, U.S.A.), anti-CTLA4, #EPR1476 (Abcam, U.S.A.) and anti-human IgG4(Abcam, U.S.A.). All antibodies were purchased from Cell Signaling Technology, Inc.
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2

Antibody Panel for Cell Analysis

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Several antibodies were used throughout this study: pan 14-3-3 pAb (Santa Cruz, K19 sc-629), K8 mAb (DSHB, 1h5), C-Cadherin mAb (DSHB, 6B6), pan Cadherin pAb (Santa Cruz, H-300 sc-10733), Vinculin mAb (Millipore, MAB3574), K19 mAb (Progen, 61010), pan keratin mAb (Sigma Aldrich, C2562), Actin-HRP (Sigma-Aldrich,A3854), GAPDH mAb (Abcam, mAbcam 9484), GFP mAb (Invitrogen, A-11120), GFP mAb (Santa Cruz, B-2 sc-9996), mCherry pAb (BioVision, 5993-100), and FLAG mAb (Sigma-Aldrich, F1804). The 1h5 anti-XCK1(8) monoclonal antibody developed by Michael Klymkowsky and 6B6 anti-C-cadherin monoclonal antibody developed by Barry Gumbiner were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the Department of Biology, University of Iowa (Iowa City, IA 52242).
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