Antibodies against β actin and rabbit igg

Z-LIG with purity more than 98 % was purchased from Chengdu Must Bio-Technology Co, Ltd (Chengdu, China) and stored in −80 °C before use. The antibodies against BRCA1, ERα, HDAC1, HDAC2, HDAC4/5/7, Acetyl-Histone3(lys9/14), IFI16, and MTA1 were purchased from Santa Cruz Biotechnology (CA, USA). Acetyl-p53, pro-PARP and cleaved PARP antibody were obtained from Cell Signaling Technology (Boston, MA, USA). The antibodies against Cyclin A, Cyclin E, CDK1, CDK2, p53, Histone 3 were obtained from Wanlei Biotechnology (Shenyang, China). The antibodies against caspase 3, cleaved caspase 3, p21 and p27 were purchased from Proteintech Group Inc (Wuhan, China). The antibodies against β-actin and rabbit IgG were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals were obtained from Sigma-Aldrich, unless indicated otherwise.

PTS extracted from Panax notoginseng was obtained from Huasun Group Co., Ltd. (Sichuan, China). As determined by HPLC in Figure 1, ginsenoside Rg1 (Rg1), notoginsenoside R1 (R1), and ginsenoside Re (Re) are the main components of PTS with the concentrations of 46.1%, 12.3%, and 5.7%, respectively. HO-1 antibody was purchased from Enzo Life Sciences (NY, USA). The antibodies against Nrf2 and lamin b1 were purchased from Santa Cruz Biotechnology (CA, USA). The antibodies against β-actin and rabbit IgG were purchased from Sigma-Aldrich (St. Louis, MO, USA). The antibody against phospho-Akt was purchased from Cell Signaling Technology (Boston, MA, USA). Other chemicals were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA) unless indicated otherwise.

VOA was extracted from Acori Graminei Rhizoma by a well-established procedure in our lab (the details described in Supplementary Methods) and stored in −80 °C before use. Four major components (β-asarone, tatarine A, β-elemene and zierone) were identified by HPLC-MS in this extract (Figures S1 and S2). The antibodies against caspase 3, cleaved caspase 3, cleaved caspase 9, LC 3II/I, atg 5 and beclin-1 were obtained from Cell Signaling Technology (Boston, MA, USA). SQSTM1/p62 antibody was obtained from Santa Cruz Biotechnology (CA, USA). The antibodies against β-actin and rabbit IgG were obtained from Sigma-Aldrich (St. Louis, MO, USA). The antibodies against p53 and cleaved caspase 8 were obtained from Wanlei Biotechnology (Shenyang, China). Other chemicals were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA) unless indicated otherwise.