For immunohistochemistry, immunofluorescence, and Western blot, the following antibodies were used according to the manufacturers' instructions: mouse anti-TNF alpha (TNF-α, ab1793; Abcam), rabbit anti-IFN-γ (bs-0480R; Bioss), anti-MPO (22225-1-AP; Proteintech), anti-alpha smooth muscle actin (α-SMA, ab124964; Abcam), anti-transferrin receptor (TFR; ab214039; Abcam), anti-GPX4 (ab125066; Abcam), anti-SLC7A11 (PA1-16893; Invitrogen), anti-GAPDH (60004-1-Ig; Proteintech), goat anti-rabbit IgG H&L (HRP; ab205718; Abcam), HRP-conjugated affinipure goat anti-mouse IgG (H+L; SA00001-1; Proteintech), and goat anti-rabbit IgG H&L (Alexa Fluor 488; ab150077; Abcam) antibodies.
Bs 0480r
Manufactured by Bioss Antibodies
Sourced in United States, China
The Bs-0480R is a laboratory equipment product from Bioss Antibodies. It is a high-performance instrument designed for specific applications. The core function of this product is to provide precise and accurate measurements, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Pa1 16893, by Thermo Fisher Scientific (1 mentions)
Ab125066, by Abcam (1 mentions)
Ab214039, by Abcam (1 mentions)
Ab124964, by Abcam (1 mentions)
Ab205718, by Abcam (1 mentions)
Sa00001 1, by Proteintech (1 mentions)
Ab150077, by Abcam (1 mentions)
Ab1793, by Abcam (1 mentions)
Ab93876, by Abcam (1 mentions)
Biotin conjugated goat anti rabbit, by Vector Laboratories (1 mentions)
Ba 1000, by Vector Laboratories (1 mentions)
Ba 9200, by Vector Laboratories (1 mentions)
Stat3, by ABclonal (1 mentions)
Peroxidase conjugated goat anti rabbit igg zb 2301, by ZSGB-BIO (1 mentions)
Peroxidase conjugated goat anti mouse igg zb 2305, by ZSGB-BIO (1 mentions)
Alexa fluor 594 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Nbp1 19371, by Novus Biologicals (1 mentions)
Ab100790, by Abcam (1 mentions)
Nbp2 29475, by Novus Biologicals (1 mentions)
5 protocols using bs 0480r
1
Comprehensive Molecular Profiling of Samples
2
Immunohistochemical Analysis of Bone and Immune Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue was fixed with 4% paraformaldehyde, embedded in paraffin, and sectioned at a thickness of 4-6 μm. Following routine procedures including quenching of endogenous peroxidase and antigen retrieval, the sections were incubated with rabbit polyclonal antibodies against osteocalcin (1:100 dilution, ab93876; Abcam, Cambridge, UK), CD3 (1:100 dilution, 17A2; Biolegend, San Diego, CA, USA), or IFN-γ (1:100 dilution, bs-0480R; Bioss, Woburn, MA, USA). After washing, the sections were incubated for 30 min with biotin-conjugated goat anti-rabbit (1:500 dilution, BA-1000; Vector, Burlingame, CA, USA) or mouse IgG (1:500 dilution, BA-9200; Vector, Burlingame, CA, USA). Tissue sections were visualized using and avidin-horseradish peroxidase (HRP) and 3,3′-diaminobenzidine (DAB), followed by counterstaining with hematoxylin. Images were acquired under a microscope.
3
Antibody Selection and Dilution for Cellular Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against PD-L1 (A11273, dilution for western blot, 1:1000; dilution for IHC analysis, 1:100; dilution for IF analysis, 1:100) and STAT3 (A11867, dilution for western blot, 1:1000; dilution for IF analysis, 1:100) were obtained from ABclonal (Wuhan, China). Antibodies against CD163 (bs-2527R, dilution for IHC analysis, 1:100), CD206 (bs-4727R, dilution for IHC analysis, 1:100), iNOS (bs-2072R, dilution for IHC analysis, 1:100) and IFNγ (bs-0480R, dilution for IHC analysis, 1:100) were obtained from Bioss (Beijing, China). Antibodies against Phospho-STAT3 (Tyr705) (9145S, dilution for western blot, 1:1000; dilution for IHC analysis, 1:100; dilution for IF analysis, 1:100; dilution for ChIP, 1:100) were obtained from Cell Signaling Technology (USA). Antibodies against β-actin (TA-09, dilution for western blot, 1:2000), peroxidase-conjugated goat anti-rabbit IgG (ZB-2301, dilution for western blot, 1:5000) and Peroxidase-conjugated goat anti-mouse IgG (ZB-2305, dilution for western blot, 1:5000) were obtained from ZSGB‐BIO (Beijing, China). Alexa Fluor 594 donkey anti-rabbit IgG (A21207, dilution for IF analysis, 1:1000) and Alexa Fluor 488 donkey anti-mouse IgG (A21202, dilution for IF analysis, 1:1000) were obtained from Invitrogen (USA).
4
Histopathological Evaluation of Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Freshly harvested tissues were fixed with phosphate-buffered formalin for a minimum of 48 h and then embedded in paraffin wax for histological analysis. The slides were stained with hematoxylin and eosin stain (H&E), and the images were captured as previously published [12 (link)]. Four to six images per section of each lung were scored blindly. For each sample, histologic evidence of pathology was classified in terms of the presence of infiltrated immune cells, lipid droplets, and foamy macrophages and was graded on a 7-point scale ranging from 0 to 6. Auramine-rhodamine (AR) staining of the lung and adipose tissue sections was performed, and the images were captured [4 (link)]. Four to six images per section of each lung were quantitated for fluorescence intensity by Image J. Immunohistochemical analysis (IHC) was performed on the formalin-fixed lung using CD4 specific rabbit polyclonal antibody (#NBP1-19371, Novus Biologicals), CD8 specific rabbit polyclonal antibody (#NBP2-29475, Novus Biologicals), F4/80-specific rabbit polyclonal (#ab100790, Abcam), and IFNγ-specific rabbit polyclonal antibody (#BS-0480R, Bioss Antibodies) as described earlier [13 (link)]. We analyzed the antibody-stained slides with semi-quantitative scoring system and graded them on a 7-point scale ranging from 0 to 6.
5
Immunofluorescent Visualization of IFN-γ in Corneal Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Corneas were enucleated, embedded in Tissue-Tek O.C.T. compound, and snap frozen in liquid nitrogen. Sections of 5 µm thickness were cut and mounted on glass slides coated with Poly-L-lysine, fixed in 4% paraformaldehyde, and blocked with PBS containing 2% BSA for 1 hour at room temperature. Then, sections were incubated with rabbit anti-mouse IFN-γ (catalog no. bs-0480R; Bioss; 1:200), followed by the Alexa Fluor 488-conjugated goat anti-rabbit IgG (catalog no. ab150077; Abcam; 1:500) secondary antibody. 4′, 6-diamidino-2-phenylindole dihydrochloride (DAPI; catalog no. 32670; Sigma-Aldrich, Darmstadt, Germany) was used to counterstain the cell nuclei. Slides were washed three times with PBS and visualized by Olympus DP72 fluorescent microscopy.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!