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Doxycyline

Manufactured by Takara Bio
Sourced in United States

Doxycycline is a broad-spectrum antibiotic that is commonly used in laboratory settings. It is a tetracycline-class drug that inhibits bacterial protein synthesis. Doxycycline is available in various formulations and can be used for a variety of applications in biological research and experimentation.

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2 protocols using doxycyline

1

Cell Culture Protocol with Selective Reagents

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The following reagents were purchased from Invitrogen (Carlsbad, CA): Dulbecco’s Modified Eagle Medium (DMEM) high glucose (cat# 11965–085), Roswell Park Memorial Institute (RPMI) 1640 (cat# 11875–085), DMEM high glucose without phenol red (cat# 31053–028), RPMI 1640 without phenol red (cat# 11835–030), Glutamax 200 mM (x100) (Cat# 35050), G418 (Geneticin® Selective Antibiotic, cat# 11811–031), and StemPro Accutase Cell Dissociation Reagent (cat# A11105–01). Tet System Approved FBS, US-Sourced (cat# 631101) and Doxycyline (cat# 631311) were purchased from Clontech (Mountain View, CA). Puromycin dihydrochloride from Streptomyces alboniger (cat# P8833–25MG) was purchased from Sigma-Aldrich (St. Louis, MO) and Steady-Glo substrate (cat# E2550) was purchased from Promega (Madison, WI).
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2

Cell Culture Protocol with Selective Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following reagents were purchased from Invitrogen (Carlsbad, CA): Dulbecco’s Modified Eagle Medium (DMEM) high glucose (cat# 11965–085), Roswell Park Memorial Institute (RPMI) 1640 (cat# 11875–085), DMEM high glucose without phenol red (cat# 31053–028), RPMI 1640 without phenol red (cat# 11835–030), Glutamax 200 mM (x100) (Cat# 35050), G418 (Geneticin® Selective Antibiotic, cat# 11811–031), and StemPro Accutase Cell Dissociation Reagent (cat# A11105–01). Tet System Approved FBS, US-Sourced (cat# 631101) and Doxycyline (cat# 631311) were purchased from Clontech (Mountain View, CA). Puromycin dihydrochloride from Streptomyces alboniger (cat# P8833–25MG) was purchased from Sigma-Aldrich (St. Louis, MO) and Steady-Glo substrate (cat# E2550) was purchased from Promega (Madison, WI).
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