Beas-2B cells were seeded into 96-well plates at a density of 5 × 103 cells/well and incubated overnight, then treated with CSE at 0, 1, 2, 4 and 6%. Cell viability was measured at different times (0, 6, 12, 24, and 36 hours) using cell counting kit-8 (CCK-8) reagent (YEASEN, China) according to the manufacturer’s instructions. After an incubation with CCK-8 reagent for 2 hours, the optical density was measured at 450 nm using a microplate reader (Bio-Rad Laboratories, USA). Cell viability was calculated by the following formula: (absorbance of treatment group/absorbance of control group) × 100%.
Cck 8 reagent
Manufactured by Bio-Rad
Sourced in United States
The CCK-8 reagent is a colorimetric assay kit used to measure cell viability and cytotoxicity. It contains a water-soluble tetrazolium salt that is reduced by living cells, producing a formazan dye that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Bio-Rad (4 mentions)
Cck 8 reagent, by Beyotime (2 mentions)
Cck 8 reagent, by Solarbio (2 mentions)
Cell counting kit 8 cck 8 reagent, by Yeasen (1 mentions)
Cell counting kit 8 cck 8 reagent, by Dojindo Laboratories (1 mentions)
Lipofectin, by Thermo Fisher Scientific (1 mentions)
Imark microplate reader, by Bio-Rad (1 mentions)
Genistein, by Merck Group (1 mentions)
Matrigel invasion chamber, by Cell Biolabs (1 mentions)
Cck 8 reagent, by Merck Group (1 mentions)
Minute plasma membrane derived lipid raft isolation kit, by Invent Biotechnologies (1 mentions)
Cell counting kit 8 cck 8 assay, by Beyotime (1 mentions)
Model 680 microplate reader, by Bio-Rad (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
11 protocols using cck 8 reagent
1
Cytotoxicity of Cigarette Smoke Extract
2
Cytotoxicity Assessment of Lipofectin and Phages
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Lipofectin (Thermo Fisher Scientific; Cat No.: 18292-037) and phage particles (T7-WT, phage 54 and phage 74) were prepared as described previously (24 (link)). In vitro cytotoxicity of Lipofectin and phage particles was evaluated using the Cell Counting Kit-8 (CCK-8) reagent (Dojindo Laboratories, Japan) according to the manufacturer’s instructions. Based on the protocol described by Zhao et al. (26 (link)), HEK293T cells were seeded in a 96 well plate at a density of 1×104 cells per well and incubated at 37°C in a 5% CO2 humidified atmosphere for 24 h. Subsequently, the medium was replaced by fresh Dulbecco’s Modified Eagle Medium (DMEM) mixed with 0, 5, 10, 15 and 20 μL Lipofectin to a final volume of 100 μL or by 100 μL fresh DMEM containing a final concentration of 0, 5×108, 1×109, 5×109 and 1×1010 pfu/mL phage particles. After a 4 h incubation, Lipofectin and phages were removed by a single wash, and cells were cultured for an additional 24-72 h. Thereafter, 10 μL of CCK-8 reagent was added and incubated for another 4 h. The absorbance at 450 nm was read with a microplate reader (iMark microplate reader, BIO-RAD). Cells without any treatment were set as 100% viability. All measurements were performed in triplicate.
3
CCK-8 Assay for Proliferation
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The proliferation of HCT116 cells was detected by performing the CCK-8 assay. Following transfection, cells (2×103 cells/well) were seeded into 96-well plates. The non-transfected cells were used as the control group. Cell proliferation was detected at 24, 48 and 72 h using the CCK-8 reagent (Beyotime Institute of Biotechnology) according to the manufacturer's instructions. Cells were incubated with 10 µl CCK-8 reagent for 2 h before the absorbance value at 450 nm was detected via a microplate reader (Bio-Rad Laboratories, Inc.).
4
Genistein's Effect on Cell Proliferation
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Cells were seeded into 96-well plates at a density of 5×103 cells per well. Different concentrations of genistein (Sigma-Aldrich) were added 12 h later. Cell proliferation was measured using a CCK-8 reagent (Solarbio Technology Co., Ltd., Beijing, China) every 24 h, following the manufacturer’s protocol, and the CCK-8 reagent was added (to a final concentration of 10%) and then incubated at 37°C for 2 h. The absorbance at 450 nm was measured using a microplate reader (iMark, Bio-Rad).
5
Cell Growth, Invasion, and Apoptosis Assays
6
Exosome-Induced HUVEC Proliferation
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Proliferation of HUVECs was measured by CCK‐8 reagent (Dojingdo Molecular Technology, Japan). HUVECs were seeded in 96‐well plates (2 × 103 cells/well) with complete ECM, and then starved for 16 hours after attachment. The cells were incubated with varying concentrations (0–100 μg/mL) of NCI‐H69‐exosomes and BEAS‐2B‐exosomes for 48 hours. Then, 10 μL of CCK‐8 reagent was added to each well for one hour and the plates were analyzed at 450 nm with a microplate reader (Bio‐Rad, Hercules, CA, USA).
7
Cell Viability Quantification using CCK-8 Assay
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Cell viability was detected using a CCK-8 assay. The cells were cultured in 96-well plates until reaching 80% confluency. Subsequently, 10 µl CCK-8 reagent (Beijing Solarbio Science & Technology Co., Ltd.) were added to each well. The cells were incubated with CCK-8 reagent for 1-4 h. The optical density value at 450 nm was detected using a microplate reader (Bio-Rad Laboratories, Inc.).
8
Cell Proliferation Assay of PC9 and PC9/GR Cells
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To assess the cell proliferation ability, a Cell Counting kit-8 (CCK-8) assay (C0038; Beyotime Institute of Biotechnology) was performed in vitro. PC9 and PC9/GR cells were implanted into 96-well plates (3×103 cells/well). At the times indicated (days 0, 1, 2 and 3), absorption at 450 nm (OD450) was determined following incubation with 10 µl CCK-8 reagent by spectrophotometry (Bio Rad Laboratories, Inc.).
9
Cell Viability Evaluation of RAW Macrophages
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Cell counting kit-8 assay (CCK-8, Beyotime Biotechnology, Shanghai, China) was performed to evaluate the viabilities of RAW macrophages after different interventions 28 (link). After treatment of ultrasound on a 6-well plate, the RAW 264.7 cells (1×103 cells/well) were seeded in a 96-well culture plate. 4 replicate wells were set in each group. 2 μl of CCK8 reagent (Bio-Rad, Hercules, CA, USA) was added to each well, incubated at 37° C for 2 h. The absorbance was measured at 450 nm.
10
Cell Proliferation Assay with CCK8 Reagent
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The CCK8 reagent (Beyotime, Shanghai, China) was used to detect cell proliferation. Cells in the exponential growth phase were seeded in 96-well plates at a density of 1 × 103. Transfection was performed when the cell density reached 60%–70%, and cell proliferation was detected 12, 24, 36, and 48 h after transfection. Before detection, 10 μL of CCK8 reagent was added to each well, and after incubation at 37 °C for 1.5 h, the absorbance at 450 nm wavelength was detected using a Model 680 Microplate Reader (Bio-Rad, CA, United States).
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