Prostaglandin estradiol (PGE2) and prostaglandin D2 (PGD2) levels were measured in culture media of ovarian explants after 1 day of exposure using an enzyme-linked immunosorbent assay (PGE2 EIA Kit: Monoclonal, #514010; PGD2: MOX ELISA Kit, #512011; Cayman Chemical Company Ann Arbor, MI, USA) according to the manufacturer’s instructions. Each sample was diluted 1:2 in sample enzyme immunoassay diluent solution prior to reactions and assayed in duplicate. Intra- and interassay coefficients of variance were 3.7% to 30.4% and 6.4% to 35% for PGE2 and PGD2, respectively.
Pgd2 mox elisa kit
Manufactured by Cayman Chemical
Sourced in United States
The PGD2: MOX ELISA Kit is a quantitative in vitro diagnostic tool used for the measurement of prostaglandin D2 metabolite (11β-PGF2α) in biological samples. The kit uses a competitive enzyme-linked immunosorbent assay (ELISA) technique to determine the concentration of the analyte.
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Lab products found in correlation
3 protocols using pgd2 mox elisa kit
1
Measuring Ovarian Prostaglandin Levels
2
PGD2 Levels in Human Scalp
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Example 6
Prostaglandin D2 Measurements in Human Scalp
Human scalp was obtained from hair transplants to use in PGD2 measurements. Haired samples were taken from donor sites of hair-bearing scalp from the occiput. Bald samples were taken from recipient tissue taken in the bald frontal scalp to create areas of placement for donor grafts. Immediately post surgery samples were placed at 4 degrees Celsius. Sample weights varied from 0.14-0.94 g. and all results were normalized to starting tissue weight. Within 1-2 days, samples were frozen in liquid nitrogen, and frozen at −70 degrees Celsius for at least 24 hours. After thawing, samples were diluted in 1 ml of acetone and homogenized using a table-top PowerGen 700 Fisher mechanical tissue grinder for 30 seconds at setting 3. Samples were vortexed, centrifuged at 5000×g for 10 minutes, with retention of the supernatant and subsequent second extraction performed on the pellet. Combined supernatants were dried in a speed-vac centrifuge, resuspended in 100 microliter (mcL) of EIA buffer, and PGD2 concentration was determined relative to a prepared standard curve using the Cayman Chemical PGD2-mox ELISA kit. Values were normalized to haired scalp for fold determination.
3
PGD2 Quantification in Biological Samples
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Briefly, lung lobes were homogenized, or supernatant was collected and then liquid-extracted using acetone. Samples were spun and washed twice and then evaporated to dryness under a stream of compressed air (about 2 min). All samples (lung samples, nasal swabs, and cell supernatant) were methyloximated to stabilize PGD2, and the assay was run as per the manufacturer's instructions. A PGD2-MOX Express ELISA kit (mouse) or a PGD2-MOX ELISA kit (human samples) was used to detect PGD2 production (Cayman Chemical). According to the manufacturer, cross-reactivity with similar molecules (for example, PGF2 or prostaglandin E2) is <1%.
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