Alexa fluor 488 goat anti mouse igg h l secondary antibody a 11001

Chloroquine diphosphate salt (CQ, C6628), bafilomycin A1 (Baf-A1, B1793), 1-(4-pyridinyl)-3-(2-quinolinyl)-2-propen-1-one (PFK-15, SML1009), Compound C (P5499), AICAR (A9978), 2-deoxyglucose (2-DG, D8375), and polyclonal antibodies against LC3 (L7543) were purchased form Sigma-Aldrich. Antibodies p44/42 MAPK (total-Erk1/2, 9102), PFKFB2 (13029), phospho-AMPKα (Thr172, 2535), total AMPKα (2532), phospho-ACC (Ser79, 3661), beta-tubulin (86298), and PARP-1 (9542) were purchased from Cell Signaling Technology. Antibodies of p62 (sc-28359) was acquired from Santa Cruz Biotechnology. Antibody of PFKFB3 (ab181861) was purchased from Abcam. Antibodies of Histone H3 (17168-1-AP) and Lamin B1 (12987-1-AP) were purchased from proteintech. Antibody against actin (TA-09) was obtained from ZhongShanJinQiao Biocompany. Alexa Fluor® 594 Goat anti-Rabbit IgG (H + L) secondary antibody (R37117) and Alexa Fluor® 488 Goat anti-Mouse IgG (H + L) secondary antibody (A-11001) were purchased from Molecular Probes. Rasfonin is stored in our lab.

Chloroquine diphosphate salt (CQ, C6628), gefitinib (Gef, SML1657), necrostatin-1 (Nec-1, 480065), N-acetyl-L-cysteine (NAC, 1009005) and polyclonal antibodies against LC3 (L7543) were purchased form Sigma-Aldrich (St. Louis, MO, USA). Cell Signaling Technology (Boston, MA, USA) provided antibodies against p-EGFR (Tyr1068, 3777), EGFR (2232) and RIP1 (3493). Purified Mouse anti-RIP (610458) was acquired from BD Transduction Laboratories (Franklin, NJ, USA). Anti-Grb2 antibodies (ab49876 and ab32037) was obtained from Abcam (Cambridge, MA, UK) and monoclonal antibody of Grb2 (sc-8034) was purchased from Santa Cruz Biotechnology. Proteintech (Wuhan, China) supplied antibody against SQSTM/p62(18420-1-AP) and EGFR (18986-1-AP). ZhongShanJinQiao Biocompany (Beijing, China) produced anti-actin (TA-09). Alexa Fluor® 488 Goat anti-Mouse IgG (H + L) secondary antibody (A-11001) were acquired from Molecular Probes (Eugene, OR, USA). MTS reagent powder (G1111) was obtained from Promega Corporation (Madision, WI, USA). ROS kit (S0033) was purchased for Beyotime (Shanghai, China). Small interference RNA (siRNA) of RIP1 (sc-36426 and L-004445-00), EGFR (sc-29301 and L-003114-00) and Grb2 (sc-29334 and L-019220-00) were obtained from Santa Cruz Biotechnology and Dharmacon along with control siRNA.