The human dental pulp cell (hDPSC) were enzymatically isolated from impacted third molars obtained from 10 adults (18 to 22 years of age), as previously described [28 (link),35 (link)], in compliance with Italian legislation (including informed consent and institutional review board approval of the protocol number 7413). The cells were cultured with α-minimum essential medium (α-MEM) supplemented with 15% fetal bovine serum (FBS), 2 mM L-glutamine, 100 mM L-ascorbic acid-2-phosphate, 100 U/mL penicillin-G, 100 mg/mL streptomycin, and 0.25 mg/mL fungizone (HyClone, Milan, Italy) and maintained in 5% CO2 at 37 °C. Proliferation, clonogenic potential, and stem cell markers were analyzed. STRO-1+ stem cells were directly sorted from pulp cell at passage 3 with mouse anti-human STRO-1 IgM (Life Technologies, Milan, Italy) with immune magnetic beads according to the manufacturer’s protocol (Dynabeads; Life Technologies, Milan, Italy). After cell sorting, each of the following experiments was performed in triplicate on pooled STRO-1 sorted cells (STRO-1+ cells).
Mouse anti human stro 1 igm
Manufactured by Thermo Fisher Scientific
Sourced in Italy
The Mouse anti-human STRO-1 IgM is a monoclonal antibody that specifically recognizes the STRO-1 antigen expressed on human mesenchymal stem cells. It can be used for the identification and isolation of STRO-1 positive cells in research applications.
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2 protocols using mouse anti human stro 1 igm
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Isolation and Characterization of Human Dental Pulp Stem Cells
2
Isolation and Sorting of STRO-1+ Dental Pulp Stem Cells
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Human dental pulp cells (hDPSC) were enzymatically isolated from unerupted third molars (adults 18-22 years of old) as described in the literature [37, (link)38] (link). The teeth were obtained in accordance with the local ethics legislation (including informed consent and institutional review board approval of the protocol number 7413). In order to obtain STRO-1 + stem cells, hDPSCs were directly sorted from pulp cell cultures at passage 3 with mouse anti-human STRO-1 IgM (Life Technologies, Milan, Italy) with immune magnetic beads, according to the manufacturer's protocol (Dynabeads; Life Technologies).
After cell sorting, each of the following experiments was performed in triplicate on pooled STRO-1-sorted cells (STRO-1 + cells).
After cell sorting, each of the following experiments was performed in triplicate on pooled STRO-1-sorted cells (STRO-1 + cells).
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