This assay follows the labelling methodology previously described in22 (link). The reaction was conducted in the presence of H2A-H2B/T122C (104 μM) with Alexa Flour 488 C5 Maleimide (558 μM (1 mg) dissolved in 200 µl DMSO) (Thermo Fisher), at room temperature for 2 h, in a reaction solution containing 10 mM Tris-HCl pH 7.5, 2 M NaCl, 1 mM EDTA, 1 mM TCEP. The reaction was terminated by the addition of 159 mM 2-mercaptoethanol, followed by dialysis against 10 mM Tris-HCl pH 7.5 buffer containing 2 M NaCl, 1 mM EDTA, and 5 mM 2-mercaptoethanol.
Alexa flour 488 c5 maleimide
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488 C5 Maleimide is a fluorescent dye used in various biological applications. It contains a maleimide functional group, which can form covalent bonds with thiol groups in proteins and other biomolecules. The dye has an excitation maximum at 494 nm and an emission maximum at 517 nm, making it suitable for detection and imaging using standard fluorescein filters.
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Lab products found in correlation
2 protocols using alexa flour 488 c5 maleimide
1
Fluorescent Labeling of H2A-H2B Histone
2
Fluorescent Labeling of H2A-H2B Histone
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