SEM was used to evaluate structures on cell surfaces, including mucus and microvilli. The samples were fixed with a 2.5% glutaraldehyde solution for 1 h, dehydrated through serial grades of ethanol (50%, 70%, 90%, and 100%) for 10 min each at room temperature, and then subjected to critical point drying (Quorum Technologies, Rewes, UK, K850) for 15–20 min. The membrane containing the cells was mounted on SEM specimen stubs. Then, the specimens were coated with gold/palladium and examined using a TESCAN MIRA3 SEM (TESCAN, Brno, Czech Republic).
Mira3 sem
Manufactured by TESCAN
Sourced in Czechia
The MIRA3 SEM is a high-performance scanning electron microscope (SEM) designed for advanced imaging and analysis. It features a field emission electron source and a variety of detectors to provide high-resolution imaging and detailed characterization of samples. The MIRA3 SEM is capable of operating in a range of vacuum conditions and can accommodate a wide variety of sample types and sizes.
Automatically generated - may contain errors
Lab products found in correlation
Octane pro sdd, by Ametek (1 mentions)
Dmi8 inverted microscope, by Leica (1 mentions)
X ray diffractometer, by Siemens (1 mentions)
Fp 750 spectrofluorometer, by Jasco (1 mentions)
Uf260, by Memmert (1 mentions)
X max 50 mm2, by Oxford Instruments (1 mentions)
Quorum q150r e, by Quorum Technologies (1 mentions)
Ntegra prima afm, by NT-MDT (1 mentions)
12 protocols using mira3 sem
1
Visualizing Cell Surface Structures
2
Characterizing Mineralized Collagen Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mineralized collagen samples were lyophilized, and a portion of each sample was mounted onto an SEM stub, which was then double carbon coated for SEM analysis. Each scaffold was imaged in a TESCAN MIRA3 SEM (Tescan, as., Brno, Czech Republic) equipped with an EDAX Octane Pro SDD energy dispersive spectrometer (EDS) at an accelerating voltage of 5 kV. EDS was performed at an accelerating voltage of 15 kV to confirm the presence of calcium and phosphate peaks from the mineral, and to assess their relative amounts with respect to the carbon peak from organic matrix. Pseudo-quantitative elemental analysis was performed from characteristic X-ray intensities to obtain Ca and P atomic % values using EDAX TEAM™ software (Pleasanton, CA, USA). Analysis of fibril morphology and diameter was performed using the ImageJ software (NIH, Bethesda, MD, USA) with 15 fibrils measured on triplicate samples for each sample condition.
3
Characterization of BaSO4 Microparticles
4
Visualizing Virus Release by SEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SEM was used to visualize virus release from the host cells. MDCK cells were grown on 12-mm poly-l -lysine-coated circular glass coverslips (Neuvitro, Vancouver, WA) in a 12-well plate at one slide per well. Cells on five slides were inoculated with the indicated virus at an MOI of 3 and then incubated at 37°C. One slide from each viral infection was washed and fixed with 2.5% glutaraldehyde at 2, 6, 8, 10, or 12 h postinfection, respectively, and postfixed with 1% OsO4. Cells were then dehydrated and critical point dried. The slides were mounted on tubs and coated with gold-palladium for examination using MIRA3 SEM (Tescan, Brno, Czech Republic).
5
SEM Sample Preparation and Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Scanning electron microscopy (SEM) was carried out on a Tescan MIRA3 SEM. Samples were freeze dried in small volumes and thin flakes were carefully mounted onto sample stubs covered in carbon conductive adhesive tapes. Each sample was then placed into a platinum sputtering system and sputter-coated to 10 nm thickness. To load the sample, the SEM chamber was vented and the sample stubs were loaded and tightened into places. After closing the sample compartment and allowing the system to reach vacuum, images were captured using operating voltage ranging from 10–30 kV.
6
Microstructural Analysis of AAMs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The microstructures of the prepared precursor and synthesized AAMs were determined using a TESCAN MIRA3 SEM. The measurement of the AAM was conducted using the paste sample with the purpose to better characterize the morphology and composition of the reaction products. Samples were coated with gold in advance of the measurement.
7
Comprehensive Characterization of Carbon Dots
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All electrochemical analyses were performed using PSTrace software and a PalmSens EmStat3 + apparatus. The Fourier-transform infrared (FTIR) experiment was carried out in the spectral range of 400–4000 cm−1 using a PerkinElmer spectrometer. The X-ray diffraction (XRD) pattern was determined using a Siemens (Germany) X-ray diffractometer with Cu Kα radiation (λ = 0.1542 nm). The energy-dispersive X-ray spectroscopy (EDS) was carried out using a TESCAN MIRA3 SEM to confirm elemental composition of the samples. Elemental analysis (CHNS) was performed on a COSTEH elemental analyzer. The TEM images were obtained by a transmission-electron microscope (LEO 906 E, Germany) at an accelerator voltage of 100 kV. Photoluminescence (PL) spectra of CDs were measured with a JASCO FP-750 Spectrofluorometer.
8
Imaging Silk Fibers and Scaffolds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Degummed fibers and silk scaffolds were imaged using an FEI Quanta 600 FEG SEM and TESCAN MIRA 3 SEM at Carnegie Mellon University’s Materials Characterization Facility. Degummed fibers and scaffolds were coated with a 5 nm layer of gold particles using a sputter coater prior to imaging, since fibroin is not naturally conductive. Images were taken using backscattered electrons at an accelerating voltage of 30 kV. Scaffolds were scanned at low magnitudes (30×) to show morphology and pore structure (n = 15), whereas fibers were scanned at higher magnifications (80× and 320×) to distinguish individual fibers for diameter measurements (n = 375) and to show any unremoved sericin residue (n = 15). Gold sputter coating and sample mounting on carbon tape rendered SEM a destructive endpoint for scaffolds.
9
Drying and SEM Analysis of CNTs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
100 ml of the stock solution (100 ppm) were dried at 50°C for 3 days in an oven (UF260, Memmert, Büchenbach, Germany). A sample, then, was sent to the University of Kashan, Kashan, Iran, for examination the composition and diameter of the tested CNTs under a scanning electron microscope (Tescan Mira3 SEM, Tescan, Fuveau, France).
10
Osteogenic Differentiation with Lyosecretome
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 24 h and 7, 14, and 21 days of osteogenic differentiation, with or without Lyosecretome, the cell/cage constructs were washed with PBS and fixed for 3 h with 3% v/v glutaraldehyde at 4 °C. Afterward, the scaffolds were dehydrated with graded ethanol series, starting with 50, 70, 90, and 100% v/v. The samples were then attached to stubs by a conductive adhesive carbon tape and metal-coated with 10 nm chromium using a high-vacuum Quorum Q150T ES Plus sputtering system. SEM imaging was performed with SEM MIRA3 (Tescan, Brno, Czech Republic) operating with an acceleration voltage of 5 kV and an EDS detector (X-max 50 mm2, Oxford Instruments, Oxford, UK). Each condition was tested in three independent experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!