Milli q purified

In total, 30 pyrrolizidine alkaloid standards were utilized in a high resolution accurate mass (HRAM) LC-MS/MS screen. Echimidine, erucifoline, europine, heliotrine, indicine, intermedine, jacobine, lasiocarpine lycopsamine, monocrotaline, retrorsine senecionine, seneciphylline, senecivernine, and their respective N-oxides, were purchased together with senkirkine and trichodesmine from Phytolab GmbH & Co. KG (Vestenbergsgreuth, Germany) and had a purity >89%. All other chemicals and solvents were of analytical reagent or HPLC grade purity. Water used for sample preparation and HPLC was Milli-Q purified (Merck Millipore, Darmstadt, Germany).

Certified standards of 30 PAs were purchased from Phytolab GmbH & Co. KG (Vestenbergsgreuth, Germany, purity > 89%) and were used in an HRAM UHPLC-MS/MS targeted screen, including echimidine, erucifoline, europine, heliotrine, indicine, intermedine, jacobine, lasiocarpine, lycopsamine, monocrotaline, retrorsine, senecionine, seneciphylline, senecivernine, and their corresponding N-oxides, as well as senkirkine and trichodesmine. All water used was Milli-Q purified (Merck Millipore, Darmstadt, Germany). Purity of all other solvents/chemicals were of analytical or HPLC grade.