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Legendplex multiplex cytokine panel kit

Manufactured by BioLegend
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Sourced in United States

The LEGENDplexTM multiplex cytokine panel kit is a bead-based immunoassay system designed to quantitatively measure multiple analytes in a single sample. The kit utilizes the unique beads coated with specific capture antibodies to enable simultaneous detection and quantification of target analytes.

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Lab products found in correlation

Legendplex analysis software, by BioLegend (2 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Facscanto 2, by BD (1 mentions)

2 protocols using legendplex multiplex cytokine panel kit

1

Multiplex Cytokine Analysis of Renal Cortical Extracts

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We used a LEGENDplexTM multiplex cytokine panel kit (BioLegend, San Diego, CA, USA) and a BD FACSCanto II flow cytometer (BD Biosciences, San Jose, CA, USA) to determine cytokine levels in the renal cortical protein extracts. These cytokines include interferon-γ (IFN-γ), interleukin (IL)-1A, IL-1B, IL-2, IL-6, and IL-17A. The fluorescent intensity of the target cytokine level was determined in each bead set by its fluorescent color code. Data generated by a flow cytometer was quantified by LEGENDplex analysis software (BioLegend). All samples were measured in duplicate.
Tain Y.L., Hou C.Y., Chang-Chien G.P., Lin S.F, & Hsu C.N. (2022). Perinatal Propionate Supplementation Protects Adult Male Offspring from Maternal Chronic Kidney Disease-Induced Hypertension. Nutrients, 14(16), 3435.
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2

Multiplex Cytokine Profiling in Renal Cortex

Cited 1 time
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Cytokines IL-1A, IL-1B, IL-2, IL-6, IL-17A, and IFN-γ in the renal cortical protein extracts were measured using a LEGENDplexTM multiplex cytokine panel kit (BioLegend, SanDiego, CA, USA) [38 (link)]. Diluted samples were measured in duplicate using a BD FACS Canto II flow cytometer (BD Biosciences, San Jose, CA, USA). The fluorescent intensity of target cytokine concentration was identified in each bead set by its fluorescent color code. The data were quantified by LEGENDplex analysis software (BioLegend, San Diego, CA, USA).
Hsu C.N., Yu H.R., Chan J.Y., Lee W.C., Wu K.L., Hou C.Y., Chang-Chien G.P., Lin S, & Tain Y.L. (2022). Maternal Acetate Supplementation Reverses Blood Pressure Increase in Male Offspring Induced by Exposure to Minocycline during Pregnancy and Lactation. International Journal of Molecular Sciences, 23(14), 7924.
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