Dimethylformamide dmf

The peptoid and peptide synthesis reagents, including bromoacetic acid, diisopropylcarbodiimide, N-methylmorophiline, methylamine, isobutylamine, and piperidine were purchased from Sigma Aldrich. Solvents and various other reagents including triethylamine, acetic anhydride, dimethylformamide (DMF), trifluoroacetic acid (TFA), triispropylsilane, diethylether, and standard biochemical reagents were purchased from Fisher Scientific. Fmoc-Gly-OH, hexafluorophosphate benzotriazole tetramethyl uronium, dichlormethane (DCM), and Fmoc-MBHA rink amide resin were purchased from VWR. The MTase-Glo methyltransferase assay kit was purchased from Promega for completing kinetic analyses. PRMT1 and PRMT5 were expressed and purified as previously described (49 (link)).

Unless stated otherwise, all of the reagents
were used as received. Glycerol monomethacrylate (GMA) and the cis-diol-capped oligo(ethylene glycol) monomethacrylate
(GEO5MA) monomer were donated by GEO Specialty Chemicals (Hythe, UK)
and used without further purification. Benzyl methacrylate (BzMA),
4,4′-azobis(4-cyanopentanoic acid) (ACVA; 99%), (3-aminopropyl)triethoxysilane
(APTES; 99%), 2-bromoisobutyryl bromide (BiBB; 98%), sodium periodate
(NaIO₄; > 99%), and dichloromethane (DCM; > 99%)
were purchased
from Sigma-Aldrich (UK). 2-Cyano-2-propyldithiobenzoate (CPDB) was
purchased from STREM Chemicals Ltd. (Cambridge, UK). N,N,N′,N″,N″-Pentamethyldiethylenetriamine
(PMDETA; 98%), tetrahydrofuran (THF), ethanol, dimethylformamide (DMF),
and hydrochloric acid (HCl, 37%) were purchased from Fisher Scientific
(UK). Sodium hydroxide pellets (NaOH, 98%) were purchased from Alfa
Aesar. Copper(II) chloride (CuCl₂; 99%) was purchased from
Acros Organics (UK). Deuterated dimethylformamide (d₇-DMF) was purchased from Goss Scientific Instruments
Ltd. (Cheshire, UK). Test-grade silicon wafers (100) were purchased
from PI-KEM (Tamworth, UK). Deionized water was used for all experiments
involving aqueous solutions.

Cerium nitrate hexahydrate (Ce(NO₃)₃·6H₂O) that was obtained from Sigma-Aldrich (Darmstadt, Germany), with a purity exceeding 99%, was used as the precursor material. Polyvinyl alcohol (PVA) with molecular weight (Mw = 85,000–124,000) and 87–89% hydrolyzed was purchased from Sigma-Aldrich (Darmstadt, Germany). PLA (molecular weight (Mw) = 420,000 g/mol and polydispersity = 1.5) was received from NatureWorks LLC (Minnetonka, MN, USA). Dichloromethane (DCM) and dimethylformamide (DMF) were purchased from Fisher Scientific, Atlanta, GA, USA. All other chemicals were of analytical grade and were used without further purification. MilliQ water (18.2  Ω cm⁻¹) was used to prepare all aqueous solutions.

The chemicals used in this work are the following: Pluronic P123 (Sigma Aldrich), tetraethyl orthosilicate (TEOS) (Sigma Aldrich, 99%), (3-aminopropyl)trimethoxysilane (APTMS) (Sigma Aldrich, ≥97%), (3-chloropropyl)trimethoxysilane (CPTMS) (Sigma Aldrich, ≥97%), sodium azide (Fisher Chemical, ≥99%), ferrocene (Sigma Aldrich, 98%), ferrocenecarboxyaldehyde (Sigma Aldrich, 98%), ethynylferrocene (Angene, 98%), toluene (Stanlab, pure p.a.), absolute ethanol (CHEMPUR, 99.8%), dimethylformamide (DMF) (Fisher Chemical, ≥99%), tert-butyl alcohol (^tBuOH) (Alfa Aesar, 99%), 2-propanol (iPrOH) (Stanlab, pure p.a.), copper(ii) sulfate pentahydrate (Sigma Aldrich, ≥99%), sodium ascorbate (Sigma Aldrich, ≥98%), zinc chloride (Alfa Aesar, ≥98%, anhydrous), glacial acetic acid (CHEMPUR, 99.9%), hydrogen peroxide (Sigma Aldrich, 25–35% w/w in H₂O, for ultratrace analysis). Deionized water was used throughout the experiments.

OsCl₃·3H₂O (99.95%) was purchased from Strem Chemicals. Dimethylformamide (DMF; 99.9%) and 200- proof ethanol was obtained from Fisher Scientific. ¹H NMR solvents CDCl₃, D₂O and Acetone-d₆ (99.9%) were obtained from Cambridge Isotope Lab Inc. The following chemicals were obtained from Sigma Aldrich: (NH₄)₂OsCl₆ (99.99%), 2,2’-bipyridine (reagent plus ⩾99%), ethylene glycol (99.9%), anhydrous diethyl ether (99.9%), hydrochloric acid (HCl; 37% w/v), 1—vinylimidazole (⩾99.9%), allylamine (AA, 98%), sodium dithionite (technical grade) and azobisisobutyronitrile (AIBN; 99%).