Purelab option q

TBZ was provided by Safic Alcan (Puteaux, France). All reagents and solvents were of analytical grade. Water used for the LC-MS analysis was prepared by using Purelab Option Q water purification system from ELGA LabWater (Antony, France).

TR hydrochloride (attested purity of 98.5%) was donated by Cristália Produtos Químicos Farmacêuticos Ltda. (Itapira, Brazil). O-Desmethyltramadol (M1), poloxamer 407 (Pluronic® F127), and poloxamer 188 (Pluronic F68) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). All other reagents were of analytical grade and deionized water from a PURELAB Option-Q (ELGA LabWater, High Wycombe, UK) water system was used for all experiments.

The Pluronic F 108NF Prill Poloxamer 338 (PL 338, molecular weight: 14,600 Da), the Pluronic F 127NF Prill Poloxamer 407 (PL 407, molecular weight: 12,600 Da), and the Pluronic L-81 (L-81, molecular weight: 2,750 Da) were products of BASF Corp. (Ludwigshafen am Rhein, Germany) purchased from Sigma-Aldrich (St. Louis, MO, USA). Levofloxacin (analytical reference material, molecular weight: 361.37 Da), methylthiazolyldiphenyl-tetrazolium bromide (MTT, molecular weight: 414.32), Brain Heart Infusion Agar (BHI Agar), and Mueller Hinton Broth were purchased from Accumedia (Neogen Corporation, Lansing, MI, USA). RPMI-1640 medium, fetal bovine serum, antibiotic/antimycotic solution (with 100 UI·mL⁻¹ penicillin and 100 μg·mL⁻¹streptomycin sulfate), PBS 1x with antibiotic/antimycotic solution, and Trypsin-EDTA were purchased from Cultilab Laboratory Materials for Growing Mobile (Campinas, São Paulo, Brazil). All other reagents were of analytical grade. Deionized water (Purelab Option-Q, ELGA LabWater, High Wycombe, UK) was used for all experiments [36 (link)].