Fgf 21 elisa kit

The conditioned medium of MSCs at passage 4 and passage 12 was collected, respectively. In brief, a total of 5 × 10⁶ MSCs were plated on a 10 cm culture plate. Twenty-four hours later, the culture medium was changed with 10 mL of serum- and antibiotic-free DMEM. After a further 24 hours, the supernatant was collected gently, filtered through a 0.22 μm filter. The concentration of FGF21 in the conditioned medium was determined by FGF-21 ELISA Kit (DF2100, R&D Systems).

A portion of liver tissue from each mouse was used for analyzing TG and total TC contents. Hepatic lipids were extracted from approximately 100 mg of liver tissue for each mouse in accordance with the method of Folch et al.38 (link). Quantification of liver and plasma TG and TC were performed using the Triglyceride E-Test and Cholesterol E-Test kits, respectively, while plasma NEFA were quantified using the NEFA C-Test kit (Wako Pure Chemical Industries, Ltd). Plasma leptin levels were quantified by enzyme-linked immunosorbent assay (ELISA) using the Leptin/mouse ELISA kit (Morinaga Institute of Biological Science, Tokyo, Japan). FGF-21 levels in plasma were measured with the FGF-21 ELISA kit (R&D System, Minneapolis, USA).

Sodium hyaluronate (HA) with different molecular weights were purchased from Bloomage Freda Biopharm. Human recombinant fibroblast growth factor basic (FGF-2), human recombinant fibroblast growth factor 21 (FGF-21), TGF beta-1 Human/Mouse Uncoated ELISA Kit, Alexa Fluor dyes, MTT cell viability reagent, PBS, penicillin-streptomycin and trypsin-EDTA were obtained from Thermo Fisher Scientific. Human FGF-2 ELISA kit, FGF-21 ELISA kit and Type I Procollagen ELISA was obtained from R&D Systems. Replica SILFLO and Ring Locator were brought from Clinical & Derm. All other reagents and solvents were purchased from Sigma-Aldrich.