Western ecl detection solution

Manufactured by Thermo Fisher Scientific

The Western ECL detection solution is a chemiluminescent reagent used in Western blotting techniques to detect and visualize target proteins. It provides a sensitive and reliable method for protein quantification and analysis.

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Lab products found in correlation

Cl xposure film, by Thermo Fisher Scientific (2 mentions) X omat 3000 ra film processor, by Kodak (2 mentions) Bis tris sds page gel electrophoresis, by Thermo Fisher Scientific (2 mentions) Donkey anti goat igg secondary antibody, by R&D Systems (2 mentions)

Close spelling variants of this product

ECL solution (151 citations) ECL detection solution (15 citations) ECL western blot detection solution (2 citations)

2 protocols using western ecl detection solution

Quantitative Western Blot Analysis of sPDGFRβ in CSF

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The quantitative western blot analysis was used to detect sPDGFRβ in human CSF (ng/mL), as we previously reported.²⁶, ²⁷ Briefly, CSF samples and recombinant human PDGFRβ carrier‐free protein (Cat. No. 385‐PR‐100/CF, R&D Systems) were subjected to 4–12% Bis‐Tris SDS/PAGE gel electrophoresis (Thermo Scientific) for 2 h at 150 V and subsequently transferred to a nitrocellulose membrane. The membrane was blocked for 1 h with a superblock blocking buffer (Cat. No. 37537, Thermo Scientific). The membrane was incubated with a primary antibody for PDGFRβ (Cat. No. AF1042, R&D Systems) overnight, then incubated with donkey anti‐goat IgG secondary antibody (Cat. No. A15999, 1:5000 dilution, Thermo Scientific) for 1 h at room temperature, treated with Western ECL detection solution (Cat. No. 34075, Thermo Scientific), exposed to CL‐Xposure film (Cat. No. 34091, Thermo Scientific), and developed in an X‐Omat 3000 RA film processor (Kodak). Images were acquired, and densitometry analysis was performed using NIH Image J software.

Cowan R.P., Gross N.B., Sweeney M.D., Sagare A.P., Montagne A., Arakaki X., Fonteh A.N., Zlokovic B.V., Pogoda J.M, & Harrington M.G. (2021). Evidence that blood–CSF barrier transport, but not inflammatory biomarkers, change in migraine, while CSF sVCAM1 associates with migraine frequency and CSF fibrinogen. Headache, 61(3), 536-545.

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Quantitative Western Blot of sPDGFRβ in CSF

Cited 1 time

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The quantitative western blot analysis was used to detect sPDGFRβ in human CSF (ng/mL), as we previously reported.^{26 (link),27 (link)} Briefly, CSF samples and recombinant human PDGFRβ carrier-free protein (Cat. No. 385-PR-100/CF, R&D Systems) were subjected to 4–12% Bis-Tris SDS/PAGE gel electrophoresis (Thermo Scientific) for 2 h at 150 V and subsequently transferred to a nitrocellulose membrane. The membrane was blocked for 1 h with a superblock blocking buffer (Cat. No. 37537, Thermo Scientific). The membrane was incubated with a primary antibody for PDGFRβ (Cat. No. AF1042, R&D Systems) overnight, then incubated with donkey anti-goat IgG secondary antibody (Cat. No. A15999, 1:5000 dilution, Thermo Scientific) for 1 h at room temperature, treated with Western ECL detection solution (Cat. No. 34075, Thermo Scientific), exposed to CL-Xposure film (Cat. No. 34091, Thermo Scientific), and developed in an X-Omat 3000 RA film processor (Kodak). Images were acquired, and densitometry analysis was performed using NIH Image J software.

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