Cells were harvested using trypsin/EDTA solution to obtain a single cell suspension. The cells were immunostained in 1% horse serum (Gibco) in sterile phosphate buffered saline (PBS) with primary antibodies conjugated with fluorescent dye. The antibodies were PerCP-conjugated anti-CD45 (Immuno Tools, Friesoythe, Germany), FITC conjugated anti-human CD44 (BD Bioscience Pharmingen, NJ, USA), PE-conjugated anti-human CD105 (Immuno Tools), and APC-conjugated anti-human CD90 (Immuno Tools). Flow cytometry analysis was performed using a FACSCalibur Flow cytometer (BD Bioscience, CA, USA).
Apc conjugated anti human cd90
Manufactured by Immunotools
Sourced in United States
APC-conjugated anti-human CD90 is a laboratory reagent used for the detection and analysis of CD90-expressing cells. CD90 is a glycosylphosphatidylinositol-anchored glycoprotein expressed on various cell types, including T cells, neurons, and hematopoietic stem cells. The APC (Allophycocyanin) fluorescent dye is conjugated to the anti-human CD90 antibody, allowing for the identification and quantification of CD90-positive cells through flow cytometry or other immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Percp conjugated anti cd45, by Immunotools (2 mentions)
Pe conjugated anti human cd105, by Immunotools (2 mentions)
Fitc conjugated anti human cd44, by BD (2 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Fascalibur, by BD (1 mentions)
Cellquest software, by BD (1 mentions)
2 protocols using apc conjugated anti human cd90
1
Multiparametric Flow Cytometry Analysis
2
Immunophenotyping of Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were detached with trypsin/EDTA solution to obtain single-cell suspension. Further, cells were washed with 1% FBS in PBS and subsequently stained with antibodies. Primary antibodies were FITC conjugated anti-human CD44 (BD Bioscience Pharmingen, USA), APC-conjugated anti-human CD90 (Immuno Tools, Germany), PE-conjugated anti-human CD105 (Immuno Tools) and PerCP-conjugated anti-CD45 (Immuno Tools). Stained cells were analysed using a FASCalibur using the CellQuest software (BD Bioscience, USA).
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