Oleanic acid

CR agonists included ACh chloride (catalogue no. A9101, Sigma-Aldrich), atractylon (catalogue no. FΑ74011, Carbosynth), $\text{β}$ -oestradiol (catalogue no. 2824, Tocris), cholesterol (catalogue no. C8667, Sigma-Aldrich), cortisone (catalogue no. C2755, Sigma-Aldrich), costunolide (catalogue no. SML.0417, Sigma-Aldrich), genistein (catalogue no, G6649, Sigma-Aldrich), glyco-diosgenin (catalogue no. GDN101. Anatrace), mecamylamine hydrochloride (catalogue no. 2843, Tocris), nootkatone (catalogue no. W316620, Sigma-Aldrich), oleanic acid (catalogue no. 05504, Sigma-Aldrich), PNU-120596 (catalogue no. 2498, Tocris), polygodial (catalogue no. 14979, Cayman Chemical Company), progesterone (catalogue no. P8783, Sigma-Aldrich), resveratrol (catalogue no. RS010, Sigma-Aldrich), ursolic acid (catalogue no, U6753, Sigma-Aldrich) and zearale none (catalogue no. 3975, Tocris). Insoluble chemicals were first reconstituted in DMSO and then diluted in ND96 to 0.1–1% final DMSO. Fish extract was prepared by btending 1 g frozen zebrafish per 2 ml of holding solution, followed by centrifugation at 3,000g for 15 min to remove debris.