Nah2po4

Manufactured by Avantor

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Sodium dihydrogen phosphate (NaH2PO4) is a chemical compound commonly used in laboratory settings. It is a crystalline, white or colorless solid that is soluble in water. NaH2PO4 serves as a buffer solution, helping to maintain a specific pH level in various experiments and applications.

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Lab products found in correlation

Na2hpo4, by Avantor (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Sodium chloride (nacl), by Avantor (3 mentions) Dmem media, by Thermo Fisher Scientific (2 mentions) Sodium azide, by Avantor (2 mentions) Tween 20, by Avantor (2 mentions) Bovine serum albumin (bsa), by Boston BioProducts (2 mentions) N hydroxysulfosuccinimide, by Thermo Fisher Scientific (2 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide, by Thermo Fisher Scientific (2 mentions) T20 cell counter, by Bio-Rad (2 mentions) Phosphate buffered saline (pbs), by Avantor (2 mentions) Nih3t3 cells, by American Type Culture Collection (2 mentions) Quikchange 2 xl mutagenesis kit, by Agilent Technologies (2 mentions) Lysogeny broth, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Avantor (2 mentions) Potassium chloride (kcl), by Avantor (2 mentions) Merlin fe sem, by Zeiss (1 mentions) Glutaraldehyde, by ProSciTech (1 mentions) Na2hpo4, by Merck Group (1 mentions)

Spelling variants of this product

NaH2PO4·H2O (4 citations) Monosodiumphosphate (NaH2PO4) (3 citations) NaH2PO4 monohydrate (2 citations) NaH2PO4.2H2O (2 citations) 0.2 M NaH2PO4 (1 citations)

19 protocols using nah2po4

Permethrin Effects on S. aureus Morphology

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To visualize any effect that permethrin had on the growth and morphology of S. aureus cells, each strain was grown in the presence of 5% permethrin, fixed, and dehydrated for imaging by scanning electron microscopy (SEM). Cells were first grown either in the presence of 5% permethrin or in MHB alone as a positive growth control and incubated on a benchtop orbital shaker for 20 hr at 35°C and 100 rpm. The cell suspension was then washed three times in 0.1 M sodium phosphate buffer (pH 7.4) (made by adding 0.1 M NaH₂PO₄ [VWR Chemicals] to 0.1 M Na₂HPO₄ [Merck]). Washed cells were then resuspended in 2.5% glutaraldehyde (ProSciTech) in 0.1 M sodium phosphate buffer and incubated overnight at 4°C to fix the cells. Following overnight incubation at 4°C, the cells were again washed three times in 0.1 M sodium phosphate buffer before being dehydrated in graded ethanol (Chem‐Supply) series (30%, 50%, 70%, 80%, 90% 10 min each and 100% for one hour). The cell suspension was then transferred to a silica wafer for imaging by SEM.
Cells were examined by SEM using a Zeiss Merlin FE‐SEM under low vacuum with a beam strength of 1 kV, an aperture of 30 µm, and with a working distance of 5 mm. A high‐efficiency secondary electron (HE‐SE2) detector was used to image cells.

Nikolic P., Mudgil P, & Whitehall J. (2020). The in vitro antibacterial effect of permethrin and formaldehyde on Staphylococcus aureus. MicrobiologyOpen, 9(8), e1054.

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Radiolabeling with [18O]-H2O

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[¹⁸O]-H₂O was from Rotem. Benzyl acrylate was from Alpha Aesar; DMSO and tetrabutylammonium bicarbonate (TBAHCO₃) from ABX; H₃PO₄ from Riedel-de Haën; Kryptofix 2.2.2. from Merck; NaH₂PO₄ and HPLC acetonitrile from VWR; and CDCl₃ and TMS from Euristop. All other reagents were from Sigma-Aldrich.

Van Hée V.F., Labar D., Dehon G., Grasso D., Grégoire V., Muccioli G.G., Frédérick R, & Sonveaux P. (2017). Radiosynthesis and validation of (±)-[18F]-3-fluoro-2-hydroxypropionate ([18F]-FLac) as a PET tracer of lactate to monitor MCT1-dependent lactate uptake in tumors. Oncotarget, 8(15), 24415-24428.

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Synthesis and Characterization of TMgP-Containing Magnesium Phosphate Cement

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TMgP (Mg₃(PO₄)₂) was synthesized by mixing MgHPO₄·3H₂O (Alfa Aeasar, Ward Hill, USA) and Mg(OH)₂ (VWR International, Radnor, USA) in a molar ratio of 2:1 and sintering for 5 h at 1100°C. The resulting sinter cake was crushed and sieved to attain particle sizes of ≤355 µm.
The amorphous magnesium phosphate cement without TMgP was prepared by mixing MgO 2835 (Magnesia GmbH) and sodium dihydrogen phosphate (NaH₂PO₄, VWR International) in a molar ratio of 2.8:1. Ultrapure water was added as a cement liquid in the respective PLR. The amorphous magnesium phosphate cement with TMgP was prepared by adding 30 mol% TMgP (MgO + TMgP = 100 mol%) to the MgO (Table 1). The molar ratio between (MgO + TMgP) and the sodium dihydrogen phosphate was maintained at 2.8:1.Table 1.

Composition of the powder of the amorphous magnesium phosphate cement with 30 mol% TMgP.

	NaH₂PO₄	MgO 2835	TMgP
Molar mass (g mol⁻¹)	120.0	40.3	262.9
Amount of substance (mmol)	10.0	19.6	8.4
Mass (g)	1.20	0.79	2.21

Kaiser F., Schröter L., Wohlfahrt P., Geroneit I., Murek J., Stahlhut P., Weichhold J., Ignatius A, & Gbureck U. (2023). Exploring the potential of magnesium oxychloride, an amorphous magnesium phosphate, and newberyite as possible bone cement candidates. Journal of Biomaterials Applications, 38(3), 438-454.

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Synthesis of Ceramic Precursors

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The following chemical precursors were used without further purification: diphosphorus pentoxide (P₂O₅, 98%, VWR, Lutterworth, UK), sodium dihydrogen phosphate (NaH₂PO₄, 99%, VWR), titanium oxide (TiO₂, 99%, VWR), calcium carbonate (CaCO₃, 98.5%, VWR) and strontium carbonate (SrCO₃, 98.5%, BDH Laboratory Supplies, Poole, UK).

Al Qaysi M., Walters N.J., Foroutan F., Owens G.J., Kim H.W., Shah R, & Knowles J.C. (2015). Strontium- and calcium-containing, titanium-stabilised phosphate-based glasses with prolonged degradation for orthopaedic tissue engineering. Journal of Biomaterials Applications, 30(3), 300-310.

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SDS-PAGE and Western Blotting Buffers

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Buffers included running buffer (20X MES SDS Running Buffer [Invitrogen] diluted to 5% in demineralized water), sample buffer (NuPAGE LDS Sample Buffer [4X], Invitrogen), transfer buffer (10 mL of 20X transfer buffer [Invitrogen] diluted to 5% in demineralized water and 20% [v/v] of 98% ethanol, pH 8.4), blocking/washing buffer (135 mM NaCl, 5 mM Na₂HPO₄ [PanReac Applichem], 1.5 mM NaH₂PO₄, 2.7 mM KCl, 0.05% Tween 20 [VWR Chemicals], pH 7.4), and sodium acetate buffer (70 mM CH₃COONa [PanReac Applichem], 30 mM CH₃COOH, pH 5.0).

Pedersen N.B., Bladbjerg E.M., Juhl C.B., Larsen A., Bloch Münster A.M., de Maat M.P, & Palarasah Y. (2024). Validation of a fibrinogen γ’ enzyme-linked immunosorbent assay using a new monoclonal antibody: effects of bariatric surgery. Research and Practice in Thrombosis and Haemostasis, 8(1), 102319.

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Structural Analysis of hFGF1 Interactions

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The Quikchange II XL mutagenesis kit was from Agilent and the DNA plasmid isolation kit was from Qiagen Inc., USA. DH5α and BL-21(DE3) competent cells were obtained from Novagen Inc., USA. Lysogeny broth is a product of EMD Millipore, USA. Heparin sepharose resin is from GE Healthcare, USA. Buffer components (Na₂HPO₄, NaH₂PO₄, NaCl, and (NH₄)SO₄) were acquired from VWR Scientific., USA. Low molecular weight (~3000 Da) heparin sodium salt was obtained from Sigma and MP Biomedicals LLC. NIH 3T3 cells were obtained from ATCC and all the cell culture reagents including, DMEM media, fetal bovine serum (FBS) and penicillin streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA). All measurements of the spatial distance between residues within hFGF1 were made using Pymol viewing software and were measured as the distance between side chain functional groups (carboxyl groups for D82 and D84, guanidinium group for R133, R82, and R84).

Davis J.E., Gundampati R.K., Jayanthi S., Anderson J., Pickhardt A., Koppolu B.P., Zaharoff D.A, & Kumar T.K. (2017). Effect of extension of the heparin binding pocket on the structure, stability, and cell proliferation activity of the human acidic fibroblast growth factor. Biochemistry and Biophysics Reports, 13, 45-57.

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Plasmid Isolation and Protein Purification

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DNA plasmid isolation kits were purchased from Qiagen, USA and Quikchange II XL mutagenesis kits were obtained from Agilent. Competent cells (DH5α and BL-21(DE3)) were sourced from Novagen Inc., USA. Lysogeny broth (LB) was obtained from EMD Millipore, USA. Heparin sepharose was obtained from GE Healthcare, USA. VWR Scientific Inc, USA was the supplier for all buffer components including Na₂HPO₄, NaH₂PO₄, NaCl, and (NH₄)SO₄. Low molecular weight heparin sodium salt (~3kDa) was procured from Sigma and MP Biomedicals LLC. NIH 3T3 cells were sourced from American Type Culture Collection (ATCC) and additional cell culture reagents such as, DMEM media, fetal bovine serum (FBS), and penicillin streptomycin were obtained from Thermo Fisher Scientific USA.

Davis J.E., Alghanmi A., Gundampati R.K., Jayanthi S., Fields E., Armstrong M., Weidling V., Shah V., Agrawal S., Koppolu B.P., Zaharoff D.A, & Kumar T.K. (2018). Probing the role of Proline -135 on the structure, stability, and cell proliferation activity of Human Acidic Fibroblast Growth Factor.. Archives of biochemistry and biophysics, 654, 115-125.

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Enzymatic Almond β-Glucosidase Analysis

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β-glucosidase from almond (Cod. G4511) and the ethyl acetate were supplied by Sigma-Aldrich. Solvents for HPLC, NMR, LC-QTOF-MS (acetonitrile, methanol, dichloromethane, dimethyl sulfoxide, deuterated chloroform) were supplied by Carlo Erba and Sigma-Aldrich. NaH₂PO₄ and Na₂HPO₄, supplied by VWR, were used to prepare phosphate buffer (50 mM, pH 6.5) for enzymatic and substrate solutions. Bicinchoninic acid kit (BCA) was used to measure protein concentration, supplied by Sigma-Aldrich.

Mazzei R., Piacentini E., Nardi M., Poerio T., Bazzarelli F., Procopio A., Di Gioia M.L., Rizza P., Ceraldi R., Morelli C., Giorno L, & Pellegrino M. (2020). Production of Plant-Derived Oleuropein Aglycone by a Combined Membrane Process and Evaluation of Its Breast Anticancer Properties. Frontiers in Bioengineering and Biotechnology, 8, 908.

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Quantifying Cellular Ribosomal RNA Content

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Ribosomal RNA content per cell was quantified by calculating 18S, 5.8S and 28S rRNA RT-qPCR signals in six biological replicates relative to the DNA content. DNA concentrations in equal volumes of papain digestion buffer (100 mM phosphate buffer (NaH₂PO₄ (VWR, Amsterdam, the Netherlands) and Na₂HPO₄ * 2H₂O (VWR), pH 6.5), 5 mM l-cysteine*HCl (Sigma-Aldrich), 5 mM EDTA (Ethylenediaminetetraacetic acid)(VWR), 33.33 μg/μl papaine (Sigma-Aldrich)) were determined in samples from day 0, 7 and 14 of ATDC5 chondrogenic differentiation using the SYBR Green assay (Invitrogen). Prior to measurement, samples were diluted in TE (Tris-EDTA) buffer (10 mM Tris/HCl pH 8 and 1 mM EDTA; day 0; 1:100 dilution, day 7 and day 14; 1:1000 dilution). A serially diluted standard curve (0.016–4 μg/ml) of calf thymus genomic control DNA (Invitrogen) in TE buffer was included to quantify the DNA concentration in the samples. Standards were prepared to contain the same amount of papain digestion buffer as the samples. SYBR Green was diluted 10,000 times in TE buffer and 100 μl was added to 100 μl of the above prepared samples and standards. After 10 min incubation fluorescence was determined using a Spectramax M2E (Molecular Devices, Sunnyvale, CA, USA) microplate reader with an excitation of 488 nm and an emission of 522 nm and DNA concentration was calculated using the standard curve.

Steinbusch M.M., van den Akker G.G., Cremers A., Witlox A.M., Staal H.M., Peffers M.J., van Rhijn L.W., Caron M.M, & Welting T.J. (2022). Adaptation of the protein translational apparatus during ATDC5 chondrogenic differentiation. Non-coding RNA Research, 7(2), 55-65.

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SARS-CoV-2 Protein Coupling to Microspheres

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SARS-CoV-2 proteins were coupled to MagPlex Microspheres of spectrally distinct regions (Luminex; Austin, TX). Coupling was carried out at room temperature following standard carbodiimide coupling procedures. Microspheres were washed once with deionized water and incubated for 20 min in the dark in an end-over-end rotator suspension of 0.1 M NaH₂PO₄ (VWR International; Radnor, PA), 5 mg/ml 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific; Waltham, MA), and 5 mg/ml N-hydroxysulfosuccinimide; Thermo Fisher Scientific). Activated microspheres were washed twice in 0.05 M MES (Boston Bioproducts, Ashland, MA) and incubated for 2 h in the dark in an end-over-end rotator, 500-μl suspension of 0.05 M MES containing 300 nmol/l Ag and 1 × 10⁶ microspheres (0.15 nmol Ag/10⁶ microspheres). Coupled microspheres were washed four times in a blocking buffer consisting of 1% BSA (Boston Bioproducts), 1× PBS (VWR International), 0.05% sodium azide (VWR International), and 0.02% Tween 20 (VWR International). Coupled microspheres were then stored at a concentration of 10⁶ spheres/ml at 4°C in the same blocking buffer. Concentrations of spheres were confirmed by counting on a Bio-Rad T20 Cell Counter.

Haddad N.S., Nguyen D.C., Kuruvilla M.E., Morrison-Porter A., Anam F., Cashman K.S., Ramonell R.P., Kyu S., Saini A.S., Cabrera-Mora M., Derrico A., Alter D., Roback J.D., Horwath M., O’Keefe J.B., Wu H.M., Wong A.K., Dretler A.W., Gripaldo R., Lane A.N., Wu H., Chu H.Y., Lee S., Hernandez M., Engineer V., Varghese J., Patel R., Jalal A., French V., Guysenov I., Lane C.E., Mengistsu T., Normile K.E., Mnzava O., Le S., Sanz I., Daiss J.L, & Lee F.E. (2021). One-Stop Serum Assay Identifies COVID-19 Disease Severity and Vaccination Responses. ImmunoHorizons, 5(5), 322-335.

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