Microstructural changes in β-LG samples were observed using a TM4000 SEM (Hitachi Co., Ltd., Tokyo, Japan) at a 5.0 kV accelerating voltage [33 (link)]. Digital images were captured at a 5.00 k× magnification, and some representative ones were presented.
Tm4000 sem
Manufactured by Hitachi
Sourced in Japan
The TM4000 SEM is a scanning electron microscope (SEM) developed by Hitachi. It is designed to provide high-quality imaging and analysis capabilities for a wide range of materials and applications. The TM4000 SEM is capable of magnifying samples up to 30,000 times and offers a variety of imaging modes and analytical tools to support various research and industrial needs.
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Lab products found in correlation
3 protocols using tm4000 sem
1
Microstructural Analysis of β-LG Samples
2
Characterizing Fibrous Scaffold Morphology
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The FR scaffolds were mounted on SEM stubs with carbon adhesive followed by sputter coating (SC7620 Mini Sputter Coater, Quorum Technologies, East Sussex, UK) to achieve a layer of gold and palladium with a thickness of 10 nm. The morphology of the FR scaffold was examined using a Hitachi TM4000 SEM (Tokyo, Japan) at 10 kV. The diameter, braiding angle, and pore size of the braiding yarns were measured using ImageJ software. For each FR scaffold, three samples were imaged and ten measurements were taken from each image.
3
Electrospun PCL-Gelatin Scaffold Characterization
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For each blend ratio, samples were mounted on a 12.7-mm scanning electron microscope (SEM) stub with carbon adhesive followed by sputter-coating (SC7620 Mini Sputter Coater; Quorum Technologies, East Sussex, UK) to achieve a layer of gold and palladium with a thickness of 10 nm. The morphology of the electrospun scaffold was examined using the Hitachi TM4000 SEM (Tokyo, Japan) at 10 kV. The diameter of fibers and pore size were measured using DiameterJ,32 (link) a plugin of ImageJ/Fiji.33 (link),34 (link) Based on the morphologic analysis results, as discussed in the Results section, only PCL-gelatin scaffolds at a 1:1 ratio were used in the subsequent experiments.
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