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Fvd efluor 506

Manufactured by Miltenyi Biotec
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FVD eFluor™ 506 is a fluorescent dye that can be used to stain and detect dead cells in flow cytometry applications. It emits fluorescence in the violet-blue region of the spectrum.

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Lab products found in correlation

Human fc block, by Miltenyi Biotec (1 mentions) Pe vio770 bdca2, by Miltenyi Biotec (1 mentions) Ecd cd123, by Beckman Coulter (1 mentions) Golgiplug, by BD (1 mentions) Cytofix cytoperm buffer, by BD (1 mentions)

2 protocols using fvd efluor 506

1

Phenotyping Plasmacytoid Dendritic Cells

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After 24 h of culture, pDCs were harvested and stained with FVD eFluor™ 506, treated with human Fc block (Miltenyi Biotec) and stained with the following antibodies: AA750-CD80, PC5.5-CD86, FITC-HLA-DR (Beckman Coulter), PE-CCR7 (Thermo Fisher Scientific), APC-programmed cell death-ligand 1 (PD-L1, BD Biosciences) or the corresponding isotype controls. The relative fluorescence intensity (RFI) ratios were calculated by normalizing the RFI of the indicated to the condition of non-activated pDCs without As2O3 treatment.
Ye Y., Ricard L., Siblany L., Stocker N., De Vassoigne F., Brissot E., Lamarthée B., Mekinian A., Mohty M., Gaugler B, & Malard F. (2020). Arsenic trioxide induces regulatory functions of plasmacytoid dendritic cells through interferon-α inhibition. Acta Pharmaceutica Sinica. B, 10(6), 1061-1072.
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2

Measuring IFN-α and TNF-α in PBMCs

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PBMCs were incubated with increasing doses of As2O3, activated with CpG-A for 6 h, and 1 μL/mL Golgi plug (BD Biosciences) was added in the last 3 h. The cells were firstly stained with FVD eFluor™ 506, then with PE-Vio770-BDCA2 (Miltenyi Biotec) and ECD-CD123 (Beckman Coulter). Cells were then stained with FITC-IFN-α and APC-Vio770-TNF-α (both from Miltenyi Biotec) with Cytofix/Cytoperm Buffer (BD Biosciences).
Ye Y., Ricard L., Siblany L., Stocker N., De Vassoigne F., Brissot E., Lamarthée B., Mekinian A., Mohty M., Gaugler B, & Malard F. (2020). Arsenic trioxide induces regulatory functions of plasmacytoid dendritic cells through interferon-α inhibition. Acta Pharmaceutica Sinica. B, 10(6), 1061-1072.
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