To investigate the effect of TGF-β1, 2×105 cells were treated with 2 ng/ml TGF-β1 (HumanZyme, Inc., Chicago, IL, USA) at 37°C for 0–4 h. To inhibit the TGF-β1 signaling pathway, 5 µM TGF-β1 inhibitor (SB431542; Selleck Chemicals, Houston, TX, USA) was added 2 h prior to the switch to high glucose. DMSO solvent was used as a control for the TGF-β1 inhibitor. The cells were harvested at different time points (0, 15 and 30 min for RT-qPCR and 0, 2 and 4 h for western blot), and the expression levels of smurf2, SnoN, TGF-β1 and Smad2 were detected using RT-qPCR and western blot analyses.
Tgf β1 inhibitor
Manufactured by Selleck Chemicals
Sourced in United States
The TGF-β1 inhibitor is a laboratory reagent that selectively inhibits the transforming growth factor beta 1 (TGF-β1) signaling pathway. It functions by blocking the activity of TGF-β1, a multifunctional cytokine involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tgf β1 inhibitor
1
TGF-β1 Signaling Pathway Analysis
2
Inhibition of TGF-β1 and STAT3 in Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB-231 and 143B cells were treated with TGF-β1 inhibitor (Selleckchem: S2805) and STAT-3 inhibitor Niclosamide (Selleckchem: S3030). The TGF- β1 inhibitor was used at 10 µM concentration, whereas STAT3 inhibitor was used at 10 µM for 24 h. Also, recombinant TGF- β1 (R&D Systems: 7754-BH-005/CF) was used at 2.5 ng/ml for 24 h. The recombinant TGF- β1 and STAT3 inhibitors were also used in combination at the same concentration for 24 h. Later, cells were harvested and utilized for qRT-PCR.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!