Fluorochrome conjugated antibodies against

Manufactured by BD

Sourced in United States, United Kingdom

Fluorochrome-conjugated antibodies are laboratory reagents used in various analytical techniques, such as flow cytometry and immunofluorescence microscopy. These antibodies are designed to bind to specific target proteins or cell surface markers, and the attached fluorescent dye (fluorochrome) allows for the detection and visualization of the labeled targets. The core function of these antibodies is to provide a reliable and sensitive method for identifying and quantifying the presence of target molecules in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Dnase, by Merck Group (1 mentions) Liberase, by Roche (1 mentions) Ack lysing buffer, by Lonza (1 mentions) Fc block human trustain fcx, by BioLegend (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Facsverse, by BD (1 mentions) Facslyric, by BD (1 mentions) Neuraminidase, by Roche (1 mentions) Live dead fixable violet dead cell stain, by Thermo Fisher Scientific (1 mentions) Ifn γ fitc, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Cytofix cytoperm, by BD (1 mentions) Perm wash, by BD (1 mentions)

3 protocols using fluorochrome conjugated antibodies against

Analyzing Immune Cells in Injured Myocardium

Check if the same lab product or an alternative is used in the 5 most similar protocols

To analyze immune cells in the injured myocardium, the infarct region was excised and digested with 50 mg/mL liberase (Roche) and 10 U DNase (Sigma) at 37°C for 30 minutes. A 40‐μm filter was then used to remove any undigested tissue. The cells were then stained with fluorochrome‐conjugated antibodies against CD45, CD11b, F4/80, Gr‐1, CD206, and EP2 (BD Biosciences or AbD Serotec) on ice for 30 minutes. For analysis of monocytes, the bone marrow cells were flushed out of the tibia and femurs with Hanks’ balanced salt solution. The peripheral blood was collected from the retro‐orbital sinus, and the red blood cells were lysed by ACK lysing buffer (Lonza). To harvest the splenocytes, the spleen was torn into small pieces with forceps, and the larger tissue pieces were removed by filtering through a 40‐μm filter. The cells were then stained with fluorochrome‐conjugated antibodies against Ly6C and CD115 (BD Biosciences) on ice for 30 minutes. Flow cytometry was performed using FACSCanto II, and the data were processed using FlowJo software.

Wu J.M., Cheng Y., Tang T.W., Shih C., Chen J, & Hsieh P.C. (2018). Prostaglandin E2 Receptor 2 Modulates Macrophage Activity for Cardiac Repair. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(19), e009216.

+ Open protocol

+ Expand

Phenotypic analysis of immune cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs, lymphocytes isolated from tissues or purified CD8⁺ T cells, were labeled using fluorescent mAbs directed against surface molecules (20 min at 4°C), washed in PBS with 0.2% BSA (Sigma-Aldrich), and acquired using FACSVerse or FACSLyric (BD Biosciences, Franklin Lakes, NJ, USA). When required, cells were blocked using FC-block (human Trustain FcX, BioLegend, San Diego, CA, USA), and viability was analyzed using the Zombie NIR or Violet viability kit (BioLegend). Cells were labeled either directly ex vivo or, where indicated, after 30 min of treatment with 25 mU neuraminidase (Roche Diagnostics, Rotkreuz, Switzerland) at 37°C. All mAbs were purchased from BioLegend, with the exception of fluorochrome-conjugated antibodies against CD3, CD8, CLA, CD45RA, LAG3, CXCR3, CCR4, and CCR7 (BD Bioscience); Siglec-9, CCR1 and CCR7 (R&D Systems, Minneapolis, USA); Siglec-7 (Beckman Coulter, Brea, CA, USA), and TNF-α (eBioscience, Waltham, MA, USA). Each mAb was titrated on PBMCs before use.

Haas Q., Markov N., Muerner L., Rubino V., Benjak A., Haubitz M., Baerlocher G.M., Ng C.K., Münz C., Riether C., Ochsenbein A.F., Simon H.U, & von Gunten S. (2022). Siglec-7 represents a glyco-immune checkpoint for non-exhausted effector memory CD8+ T cells with high functional and metabolic capacities. Frontiers in Immunology, 13, 996746.

+ Open protocol

+ Expand

Cytokine Profiling of Stimulated T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess cytokine production by T cells, stimulated PBMC were incubated with PMN at the indicated ratio. Cells were co-cultured for a total of 16 hours with the addition of brefeldin A at a final concentration of 10 μg/ml after the initial hour. Cells were washed and surface stained with fluorochrome-conjugated antibodies against CD4 (Clone SK4), CD8 (Clone RPA-T8) (BD bioscience) and Live/Dead Fixable Violet dead cell stain (Invitrogen, UK). Subsequently, the cells were washed, treated with Cytofix/Cytoperm and Perm/Wash (BD Biosciences) according to manufacturer instructions, and then stained intracellularly with IFN-γ-FITC (Clone B27, BD biosciences), IL-2–PE (Clone MQ1-17H12) and TNF-α-PerCP-Cy5.5 (Clone Mab11) (both eBioscience, San Diego). Data were acquired on the LSRFortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software.

Yang T.H., St. John L.S., Garber H.R., Kerros C., Ruisaard K.E., Clise-Dwyer K., Alatrash G., Ma Q, & Molldrem J.J. (2018). Membrane-Associated Proteinase 3 on Granulocytes and Acute Myeloid Leukemia Inhibits T Cell Proliferation. The Journal of Immunology Author Choice, 201(5), 1389-1399.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!