Aliquots of cell lysate (50 µg/lane) were separated by electrophoresis on 10% or 12% SDS polyacrylamide gels. Anti-phospho-STAT6, anti-STAT6, anti-phospho-SMAD2 (#3104), anti-SMAD2, anti-phospho-SMAD3, anti-SMAD3, anti-phospho-SMAD1, 5, and 9, anti-SMAD1, 5, and 9, anti-phospho-ERK1/2 (44/42 MAPK) (#4370), anti-ERK1/2 (#9102), anti-phospho-p38, anti-p38, anti-phospho-JNK, anti-JNK, and anti-SDHA (#5893) antibodies were purchased from Cell Signaling Technology (Beverley, MA, USA). Anti-NDUFA9 and COX-4 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-UQCRC2 (ab14745) was purchased from Abcam (Cambridge, UK). Anti-ATP5A1 was purchased from Invitrogen (Thermo Fisher). Secondary antibodies (goat anti-mouse and goat anti-rabbit) were obtained from Santa Cruz Biotechnology. Images were scanned on an ODYSSEY instrument and quantified using Image Studio Digits (LI-COR Biosciences, Lincoln, NE, USA). Full length uncropped blots are presented in Supplementary Figures 12 –17 .
Anti ndufa9
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-NDUFA9 is a laboratory reagent used for the detection and quantification of the NDUFA9 protein. NDUFA9 is a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which is the largest of the respiratory complexes.
Automatically generated - may contain errors
Lab products found in correlation
Anti p38, by Cell Signaling Technology (1 mentions)
Image studio digits, by LI COR (1 mentions)
Anti stat6, by Cell Signaling Technology (1 mentions)
Anti phospho stat6, by Cell Signaling Technology (1 mentions)
Anti sdha, by Cell Signaling Technology (1 mentions)
Anti atp5a1, by Thermo Fisher Scientific (1 mentions)
Anti uqcrc2 ab14745, by Abcam (1 mentions)
Anti phospho smad2, by Cell Signaling Technology (1 mentions)
Anti phospho smad3, by Cell Signaling Technology (1 mentions)
Anti smad2, by Cell Signaling Technology (1 mentions)
Anti smad3, by Cell Signaling Technology (1 mentions)
Anti erk1 2 9102, by Cell Signaling Technology (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
Anti jnk, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti cox 4, by Santa Cruz Biotechnology (1 mentions)
Anti atp5a, by Abcam (1 mentions)
Anti uqcrc2, by Abcam (1 mentions)
Bradford assay, by Cell Signaling Technology (1 mentions)
Anti mnsod, by Abcam (1 mentions)
2 protocols using anti ndufa9
1
Protein Quantification and Validation Methodology
2
Mitochondrial Antioxidant and OXPHOS Protein Analysis
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Total protein concentration was determined using the Bradford assay (Cell Signaling Technology, Denver, MA, USA). Samples were resolved in 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis, transferred to 0.45-μM polyvinyl difluoride membranes, and analyzed separately. After blocking with 5% skim milk at room temperature for 60 minutes, the blots were surveyed with primary antibodies. Expression of mitochondrial antioxidant was detected using anti-Mn-SOD (Abcam, Cambridge, MA, USA). We used anti-NDUFA9 (Santa Cruz Biotechnology, Dallas, TX, USA), anti-SDHB (Abcam), anti-UQCRC2 (Abcam), anti-COXIV (Santa Cruz Biotechnology), and anti-ATP5A (Abcam) to detect the expression of oxidative phosphorylation complexes.
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