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2 protocols using anti phosho erk1 2

1

Comprehensive Antibody Panel for Alzheimer's Research

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anti-Aβ/APP 4G8 (mouse mAb, Covance), anti-Aβ/APP 6E10 (mouse mAb, Covance), anti-AβOs scFvA13 and scFvIm3 (ref. 13 (link)), anti-oligomer A11 (ref. 21 (link)) (rabbit pAb Millipore), anti-Aβ (rabbit mAb, Cell Signaling), anti α-Synuclein (mouse mAb, Abcam), anti-APP C-terminal fragment (rabbit pAb, Sigma), anti-APP N-terminal 22C11 (mouse mAb, Millipore), anti-V5 (mouse mAb Invitrogen, and rabbit pAb Sigma), anti-V5-HRP (mouse mAb, Sigma), anti-His tag (mouse mAb Millipore), anti-KDEL (mouse mAb, Assay Design/Stressgen) (kind gift of Prof. R. Sitia, HSR San Raffaele, Milan), anti-mTOR (rabbit pAb, Cell Signaling), anti-phospho-mTOR (rabbit pAb, Cell Signaling), anti-P70S6K (rabbit pAb, Cell Signaling) (kind gift of Dr G. Amadoro, CNR, Rome), anti-phospho-P70S6K (rabbit pAb, Cell Signaling), anti ERK1/2 (rabbit pAb, Cell Signaling), anti phosho-ERK1/2 (rabbit pAb, Cell Signaling), anti phospho-CREB (rabbit pAb, Cell Signaling), anti β-Actin (mouse mAb and rabbit pAb, Sigma), anti-calnexin (rabbit pAb, Sigma), anti-GM130 (mouse mAb, Covance), anti-Golgi 58K (mouse mAb, Sigma), anti-Rab3A (mouse mAb, Sigma), anti-Rab5 (rabbit pAb, Abcam), anti-Rab7 (goat pAb, Santa Cruz), anti-TGN46 (rabbit, pAb), anti-LAMP-1 (rabbit pAb, Abcam). anti-Rab5, anti-Rab7, anti-TGN46 are kind gift of Dr V. Triaca, CNR, Rome.
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2

Western Blot Analysis of Protein Markers

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Lung and cell culture protein lysates (30 μg/lane) were separated by 10–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunoblots were developed as previously described in [16 (link)]. The blots were probed with one of the following antibodies: anti-AnxA1 (1:1000, Bioss Inc., Woburn, MA, USA), anti-Bcl-2, anti-lamin B1 (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), anti-NF-κB p65, anti-phospho-NF-κB p65, anti-IκB-α, anti-ERK1/2, anti-phosho-ERK1/2, anti-JNK, anti-phospho-JNK, anti-p38, anti-phospho-p38, anti-MKP-1 (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-TATA (1:1000, Abcam, Cambridge, MA, USA), or β-actin (1:10,000, Sigma Chemical Company, St. Louis, MO, USA). The data were presented as the relative ratio of the target protein to the reference protein. The relative ratio of the target protein of the control group was arbitrarily expressed as 1.
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