The chemicals in this study were of analytical grade and were used as received without further purification. PTA sodium salt from rice (molecular formula: C6H18O24P6 xNa+·yH2O; molecular weight: 660.04) with ≥90 purity and CS, deacetylated at 75%–85%, sodium amp salt (>98% purity; molecular weight: 371.4 g/mol) were acquired from Sigma-Aldrich (St Louis, MO, USA). Aqueous acetic acid solution (99.8%) was supplied by Hamburg Industries (Hamburg, Germany). Ferrous chloride tetrahydrate (FeCl2·4H2O, ≥99% purity) and ferric chloride hexahydrate (FeCl3·6H2O, ≥99% purity) were obtained from Merck KGaA (Darmstadt, Germany). Ammonia solution (25%) was purchased from Scharlau (Sentmenat, Barcelona, Spain). Deionized water was used in each experiment.
Sodium amp salt
Manufactured by Merck Group
Sourced in United States
Sodium salt is a common chemical compound used in various laboratory applications. It serves as a source of the sodium ion, which is an essential element in many chemical reactions and biological processes. The core function of sodium salt is to provide the sodium ion for use in experiments, analyses, and other laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Ferrous chloride tetrahydrate, by Merck Group (2 mentions)
Ferric chloride hexahydrate, by Merck Group (2 mentions)
Ammonia solution, by Scharlab (1 mentions)
Pyrazinamide, by Merck Group (1 mentions)
4 amino salicylic acid, by Merck Group (1 mentions)
Isonicotinic acid hydrazide, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
4 protocols using sodium amp salt
1
Synthesis and Characterization of Metal-Organic Complexes
2
Synthesis and Characterization of Chitosan-Based Composites
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The chemicals in this study were reagent grade and were used as received without purification. Ferrous chloride tetrahydrate (FeCl2·4H2O), ferric chloride hexahydrate (FeCl3·6H2O), and sodium hydroxide (NaOH) were purchased from Merck KGaA (Darmstadt, Germany). Chitosan, deacetylated at 75%–85%, sodium amp salt (>98% purity; molecular weight 371.4 g/mol), 4-amino salicylic acid, isonicotinic acid hydrazide, and pyrazinamide were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Acetic acid (99.8%) was purchased from Hamburg Industries (Hamburg, Germany). Deionized water was used in each experiment.
3
Synthesis and Characterization of Luciferyl-Adenylate
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The luciferyl-adenylate was prepared using LH2 acid and AMP sodium salt (SIGMA) by following a previously described preparation procedure for D-luciferyl-adenylate [46 (link)]. Luciferyl-adenylate was analyzed with silica gel TLC (moving phase: ethyl acetate/ethanol/water (5:3:2), followed by revelation by fluorescence with a UV lamp. Luciferyl-adenylate displayed yellowish fluorescence with an Rf = 0.68 (Rf(luciferin) = 0.87 with greenish fluorescence). A luciferyl-adenylate concentration was estimated from stoichiometric amounts of luciferin and ATP used for its synthesis. According to such estimations, luciferyl-adenylate concentration in stock solution are in the range between 5 to 10 mM.
4
Antibiotic Stock Solution Preparation
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CRO disodium salt hemi(heptahydrate) (Sigma, St. Louis, MO), ETP sodium salt (Research Products International, Prospect, IL), and MEM trihydrate (TCI, Portland, OR) were used to create 1.0 mg/mL antibiotic stock solutions in NF-H2O based on manufacturer-reported purity, aliquoted, and stored at −80°C. AMP sodium salt (Sigma, St. Louis, MO) was used to create 10.0 mg/mL antibiotic stock solutions in NF-H2O based on manufacturer-reported purity, aliquoted, and stored at −80°C. Aliquots were only thawed and used once on the days of experiments.
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