Ldl uptake assay kit
The LDL Uptake Assay kit is a laboratory tool designed to measure the uptake of low-density lipoprotein (LDL) by cells. The kit provides the necessary reagents and protocols to quantify the cellular internalization of fluorescently-labeled LDL particles.
3 protocols using ldl uptake assay kit
Evaluating iPSC-Derived Hepatocyte Functions
Quantifying Metabolic Markers in Adipocytes
Glycerol released from mature 3T3-L1 cells into the medium was quantified using a glycerol assay kit (#MAK117, Sigma-aldrich, St. Louis, MI, USA). Briefly, the amount of glycerol released into the medium following the various treatments was measured by adding a reaction mixture, and the absorbance was measured at 570 nm.
LDL uptake was measured using an LDL uptake assay kit (#10011125, Cayman Chemicals, Ann Arbor, MI, USA). Briefly, cells were seeded in a black plate and stained with LDL-DyLightTM solution in serum-free medium for 24 h. After removing the reaction medium, images were captured using a fluorescence microscope, and the fluorescence intensity was quantified using ImageJ (version 1.54d, NIH, Bethesda, MD, USA).
Quantifying LDL Uptake in HepG2 Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!