The largest database of trusted experimental protocols

Ldl uptake assay kit

Manufactured by Cayman Chemical
Sourced in United States

The LDL Uptake Assay kit is a laboratory tool designed to measure the uptake of low-density lipoprotein (LDL) by cells. The kit provides the necessary reagents and protocols to quantify the cellular internalization of fluorescently-labeled LDL particles.

Automatically generated - may contain errors

3 protocols using ldl uptake assay kit

1

Evaluating iPSC-Derived Hepatocyte Functions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ability of the iPSC-HLCs to both uptake LDL and secrete albumin was studied using the LDL Uptake Assay kit (Cayman Chemicals, USA) and Human Albumin ELISA Quantitation set (Bethyl Laboratories, USA), respectively, according to the manufacturers' instructions. Secreted albumin was measured from 24-h conditioned medium and normalised to the cell number.
+ Open protocol
+ Expand
2

Quantifying Metabolic Markers in Adipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glucose concentration was measured using a glucose assay kit (#ab136955, Abcam). Briefly, the amount of glucose released into the culture medium by the cells was measured by adding the reaction mixture and incubating. Glucose concentration was determined by measuring the absorbance at 450 nm.
Glycerol released from mature 3T3-L1 cells into the medium was quantified using a glycerol assay kit (#MAK117, Sigma-aldrich, St. Louis, MI, USA). Briefly, the amount of glycerol released into the medium following the various treatments was measured by adding a reaction mixture, and the absorbance was measured at 570 nm.
LDL uptake was measured using an LDL uptake assay kit (#10011125, Cayman Chemicals, Ann Arbor, MI, USA). Briefly, cells were seeded in a black plate and stained with LDL-DyLightTM solution in serum-free medium for 24 h. After removing the reaction medium, images were captured using a fluorescence microscope, and the fluorescence intensity was quantified using ImageJ (version 1.54d, NIH, Bethesda, MD, USA).
+ Open protocol
+ Expand
3

Quantifying LDL Uptake in HepG2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monolayers of HepG2 cells were incubated with 50 μg/mL fresh, isolated, nonacetylated human LDL labeled with Dil (Invitrogen) for 1 hour in the dark with serum-free medium. Cells were trypsinized to obtain a single-cell suspension and Dil-LDL uptake was quantified by flow cytometry (BD Accuri C6 flow cytometer). LDL Uptake Assay Kit (Cayman) was used to evaluate LDL uptake. Briefly, cells were incubated with 5 μg/mL LDL-DyLight 550 working solution prepared in serum-free medium for 12 hours, then fixed and imaged under a fluorescence microscope (Olympus FV1000 Spectral Confocal).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!