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Salvinorin b

Manufactured by Cayman Chemical
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Salvinorin B is a chemical compound that is a naturally occurring diterpene found in the Mexican plant Salvia divinorum. It is the primary active constituent of the plant, responsible for its psychoactive effects. Salvinorin B is a potent and selective kappa opioid receptor agonist.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions) Ghrelin, by Peptide Institute (1 mentions)

4 protocols using salvinorin b

1

KORD Actuator Salvinorin B Protocol

Cited 1 time
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The KORD actuator salvinorin B (SALB; Cayman Chemical, Ann Arbor, MI) was prepared as described previously (Tuscher et al., 2018 (link)). SALB was dissolved in 100% DMSO (Fisher Scientific, Pittsburg, PA) to a concentration of 10 mg/mL, aliquoted, and stored at −20 °C. On the day of injection, an aliquot was thawed, briefly warmed to 40 °C, and vortexed to ensure that the compound was fully dissolved. SALB was injected intraperitoneally (i.p.) at a dose of 10 mg/kg. Post-training i.p. injection of this SALB dose impairs object placement memory consolidation in ovariectomized mice expressing the KORD in the dorsal hippocampus (Tuscher et al., 2018 (link)). Mice were weighed 1–2 days prior to behavioral testing to avoid additional stress on the testing day.
Schwabe M.R., Lincoln C.M., Ivers M.M, & Frick K.M. (2021). Chemogenetic inactivation of the nucleus reuniens impairs object placement memory in female mice. Neurobiology of learning and memory, 185, 107521.
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2

Sourcing Reagents for Experiments

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Otherwise stated, all chemical were purchased from Sigma Aldrich (St. Louis. MO, USA). Dln101 and ghrelin were obtained from Peptides International (Louisville, KY, USA). CNO was obtained from Enzo Life Sciences and Salvinorin B from Cayman chemicals.
Stutz B., Nasrallah C., Nigro M., Curry D., Liu Z.W., Gao X.B., Elsworth J.D., Mintz L, & Horvath T.L. (2019). Dopamine neuronal protection in the mouse Substantia nigra by GHSR is independent of electric activity. Molecular Metabolism, 24, 120-138.
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3

KORD Actuator Salvinorin B Protocol

Cited 1 time
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The KORD actuator salvinorin B (SALB; Cayman Chemical, Ann Arbor, MI) was prepared as described previously (Tuscher et al., 2018 (link)). SALB was dissolved in 100% DMSO (Fisher Scientific, Pittsburg, PA) to a concentration of 10 mg/mL, aliquoted, and stored at −20 °C. On the day of injection, an aliquot was thawed, briefly warmed to 40 °C, and vortexed to ensure that the compound was fully dissolved. SALB was injected intraperitoneally (i.p.) at a dose of 10 mg/kg. Post-training i.p. injection of this SALB dose impairs object placement memory consolidation in ovariectomized mice expressing the KORD in the dorsal hippocampus (Tuscher et al., 2018 (link)). Mice were weighed 1–2 days prior to behavioral testing to avoid additional stress on the testing day.
Schwabe M.R., Lincoln C.M., Ivers M.M, & Frick K.M. (2021). Chemogenetic inactivation of the nucleus reuniens impairs object placement memory in female mice. Neurobiology of learning and memory, 185, 107521.
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4

Inhibition of AgRP Neurons in Mice

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Peripubertal mice harboring at least one copy of an AgRP-Cre transgene were anesthetized with a ketamine/xylazine cocktail and injected bilaterally into the ARC (Bregma distance – X: ± 0.25; Y: −1.4; Z: −5.8) with 300nL of inhibitory DREADD virus (rAAV9-hSyn-dF-HA-KORD-IRES-mCitrine, 5x1012 genome copies (GC) per ml) (UNC Vector Core). Mice were allowed to recover for approximately ten days and then injected with salvinorin B (Cayman Chemical) or DMSO vehicle control (5mg/kg s.c. daily) for inhibitory experiments, for ten consecutive days.
Stutz B., Waterson M.J., Šestan-Peša M., Dietrich M.O., Škarica M., Sestan N., Racz B., Magyar A., Sotonyi P., Liu Z.W., Gao X.B., Matyas F., Stoiljkovic M, & Horvath T.L. (2022). AgRP neurons control structure and function of the medial prefrontal cortex. Molecular psychiatry, 27(10), 3951-3960.
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