Mice were bled into 150 µL of Alsever’s solution. Samples were then treated with 10 mL ACK buffer for 2 min and centrifuged at 1600 RPM for 5 min. The supernatant was removed, and pellets were washed in 5 mL sample media (SM, PBS with 2 mM EDTA and 2% FCS). Antibodies of Biolegend (San Diego, CA, USA): CD45.1 APC, CD45.2 pacific blue, Mac1 PE-Cy7, B220 APC-Cy7, CD3e PE, Lineage-PacificBlue (Including- anti Ter119, Mac1, Gr1, CD3e, CD4, CD8 and B220), c-Kit Alexa780, Sca1-APC, CD150-PEcy7, CD48- Percp cy5.5, CD9-APC, CD34-PE, CD84-PE. Cells were stained on ice for 1 hr and washed. Flow cytometry analysis of the reporter expression frequencies (ZsGreen+) and the surface markers’ expression were performed on the Gallios Flow Cytometer (Beckman Coulter, Brea, CA, USA). Fluorescence activated cell sorting (FACS) data analysis was performed using Kaluza v1.2 (Beckman Coulter). Sorting was performed on FACSAria III (BD), as previously reported [9 (link)].
Cd45.1 apc
Manufactured by BioLegend
Sourced in United States
CD45.1-APC is a fluorochrome-conjugated antibody that binds to the CD45.1 surface antigen. CD45 is a tyrosine phosphatase that is expressed on the surface of most hematopoietic cells and plays a role in the regulation of cell growth and differentiation. The APC (Allophycocyanin) fluorochrome attached to the CD45.1 antibody allows for the detection and analysis of CD45.1-positive cells using flow cytometry.
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Lab products found in correlation
Sca1 apc, by BioLegend (4 mentions)
Cd3e pe, by BioLegend (3 mentions)
C kit apccy7, by BioLegend (3 mentions)
Facsaria 2, by BD (2 mentions)
Cd45 pe, by BioLegend (2 mentions)
Cd14 fitc, by BioLegend (2 mentions)
Cd34 apc, by BioLegend (2 mentions)
Cd15 pacific blue, by BioLegend (2 mentions)
Glycophorin pecy5, by BioLegend (2 mentions)
Clone hir2, by BioLegend (2 mentions)
Mac 1 cd11b pecy7, by BioLegend (2 mentions)
Gr 1 pe, by BioLegend (2 mentions)
B220 pecy5, by BioLegend (2 mentions)
Cd45.2 fitc, by BioLegend (2 mentions)
Cd11b, by BioLegend (2 mentions)
B220 apc cy7, by BioLegend (2 mentions)
Mac1 pecy7, by BioLegend (2 mentions)
Cd45.2 pacblue, by BioLegend (2 mentions)
Facsaria 3, by BD (1 mentions)
Kaluza v1, by Beckman Coulter (1 mentions)
5 protocols using cd45.1 apc
1
Murine Hematopoietic Cell Isolation
2
Hematopoietic Progenitor Cell Analysis
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Cells were suspended in Ca++ Mg++-free phosphate-buffered saline (PBS) containing 2% (vol/vol) FBS (Globalstem). Human hematopoietic progenitors cells were identified by labeling with CD45-PE (1:50; clone HI30) and or CD34-APC (1:50; clone 561) while mature phenotypes were analyzed using CD15-Pacific Blue (monocytes and granulocytes) (1:50; clone W6D3/HI98), CD14-FITC (monocytes and macrophages)(clone 1:50; M5E2); CD11b (1:50; ICRF44) (macrophages) and Glycophorin-PECy5 (red blood cell membrane), and erythroid precursors (1:50; Clone HIR2) (all from Biolegend). Murine hematopoietic cells were identified using the following antibodies: Mac-1/CD11b-PECy7 (1:200; clone M1/70), Gr-1-PE (1:200; clone 8C5), B220-PECy5 (1:100; clone RA3-6B2), Sca1-APC (1:100; clone E13-161-7), c-Kit-APCCy7 (1:100; clone 2B8): CD45.1-APC (1:100; clone A20) and CD45.2-FITC (1:100; clone 104) (all from Biolegends). All antibodies were incubated at the concentration suggested by the manufacturer for 30 minutes on ice. Non-specific signals and dead cells were excluded by appropriate fluorochrome-conjugated isotype and propidium iodide staining, respectively. Cell fluorescence was analyzed with the FACSAria II (Becton Dickinson).
3
Hematopoietic Progenitor Cell Analysis
4
Mice Tail Bleed and Blood Panel Staining
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Mice were tail bled into a 150-µl Alsever’s solution. Samples where then treated with 10 ml Ammonium-Chloride-Potassium (ACK) Lysing Buffer for 2 min and centrifuged (1600 rpm) for 5 min. The supernatant was removed, washed in 5 ml SM, centrifuged again, and finally plated in a 96-U well for 1–3 h for staining. Blood panel staining: CD45.1-Apc, CD45.2-PacBlue, Mac1-PeCy7, B220-ApcCy7, and CD3e-PE (all from Biolegend, San Diego, CA).
5
Comprehensive Immune Cell Profiling
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Antibodies used: CD45.1-Apc, CD45.2- PacBlue, Ter119-PerCPCy5.5, Mac1-PeCy7, B220-ApcCy7, CD3e-PE, cKit-ApcCy7, Sca1-APC, CD150-PeCy7, CD48--PerCPCy5.5, Gr1-PacBlue, and CD19-APC (all from Biolegend, San Diego, CA). Cells were stained on ice for 30 min, washed, filtered through a 70-µm filter, and analyzed using Fluorescence-Activated Cell Sort machine (FACS). Routine analysis was performed by using 4-laser Gallios (Beckman Coulter) and the Kaluza analysis software (Version 2.1). Sorting used of a 6-laser, BD FACSAria™ III sorter.
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