Horseradish peroxidase diaminobenzidine detection ihc kit

The samples were prepared for indirect immune detection using a rabbit polyclonal anti-BMP-2 (Abcam, Cambridge, MA, USA) and mouse and rabbit specific horseradish peroxidase/ diaminobenzidine detection IHC kit (Abcam, Cambridge, MA, USA) by protocols provided by manufacture. The sections were then counterstained with hematoxylin to visualize cell nuclei. BMP-2 were stained brown [20 (link)]. Under high power magnification, the BMP-2 stain in the callus area was measured and quantified [20 (link),29 (link)].

Clinical specimens and tumor xenograft specimens were fixed in 10% formalin and embedded in paraffin. We cut the paraffin-embedded specimens into sections of 5.0 μm and stained with hematoxylin and eosin (HE) for general morphological observation. Immunohistochemical (IHC) staining was performed using a rabbit-specific horseradish peroxidase/diaminobenzidine detection IHC kit (Abcam, Cambridge, UK) according to the manufacturer's protocol. Tissue sections were stained with anti-B7H3 (Abcam, Cambridge, UK, 1:200 dilution) for 30 min at room temperature, followed by incubation with secondary rabbit-specific HRP-conjugated antibodies for B7-H3 staining. Samples were visualized using a Nikon Eclipse 80i upright microscope (Nikon, Tokyo, Japan).