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Hsc 3 m3

Manufactured by RIKEN BioResource Center
Sourced in Japan

The HSC-3-M3 is a laboratory equipment designed for high-speed cell sorting. It is a compact, multi-parameter flow cytometer capable of analyzing and separating cell populations based on their physical and fluorescent characteristics.

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2 protocols using hsc 3 m3

1

Oral Squamous Cell Carcinoma Cell Lines

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A total of 7 human OSCC-derived cell lines (HSC-2, HSC-3, HSC-3-M3, Ca9-22, Sa3, Ho-1-u-1, and Ho-1-N-1) were purchased from RIKEN BioResource Center (Tsukuba, Japan) and the Japanese Collection of Research Bioresources Cell Bank (Ibaraki, Japan). OSCC-derived cells were cultured as previously described [15 (link)]. We obtained human normal oral keratinocytes (HNOKs) from healthy young volunteer patients. HNOKs were cultured as previously described and used as normal controls [16 (link)].
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2

Comparative analysis of OSCC cell lines

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Eleven human OSCC-derived cell lines (HSC-2, HSC-3, HSC-3-M3, HSC-4, Sa3, Ca9-22, KOSC-2, SAS, SAS-H1, Ho-1-u-1, and Ho-1-N-1) were purchased from RIKEN BioResource Center (Tsukuba, Ibaraki, Japan) and the Japanese Collection of Research Bioresources Cell Bank (Ibaraki, Osaka, Japan) 11 (link)-13 (link). We obtained human normal oral keratinocyte (HNOKs) from young healthy patients and cultured the cells as described previously 14 -17 . The cells used in this study were within 10 passages from thawing. They also were routinely tested using an EZ-PCR Mycoplasma Test Kit (Biological Industries, Kibbutz Beit Haemek, Israel).
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